Sains Malaysiana 44(8)(2015): 1103–1110
Identification of Indigenous Bacteria Isolated from Shrimp
Aquaculture Wastewater with Bioremediation Application: Total Ammoniacal
Nitrogen (TAN) and Nitrite Removal
(Pengenalpastian
Bakteria Asli Dipencilkan daripada Air Sisa Udang Akuakultur dengan
Aplikasi BioPemulihan: Jumlah Amonia Nitrogen (TAN)
dan Penyingkiran Nitrit)
SARMILA
MUTHUKRISHNAN1,3,
VIKINESWARY
SABARATNAM1,2,
GEOK-YUAN
ANNIE
TAN1,2,3
& VING-CHING CHONG1*
1Institute of Biological
Sciences, Faculty of Science, University of Malaya, 50603 Kuala
Lumpur
Malaysia
2Mushroom Research Centre,
Institute Biological Sciences, Faculty of Science, University of
Malaya
50603
Kuala Lumpur, Malaysia
3Institute of Ocean and
Earth Sciences (IOES), University of Malaya, 50603 Kuala Lumpur
Malaysia
Diserahkan: 10 Ogos 2014/Diterima:
24 Mac 2015
ABSTRACT
Deterioration of water
quality mainly due to high total ammonia nitrogen (TAN)
and nitrite will affect the productivity of shrimp culture. In this
study, three indigenous strains assigned as VCM5, VCM8
and VCM12 were evaluated for their ability to degrade TAN
and nitrite. These strains were inoculated into shrimp
aquaculture wastewater to enhance the degradation of TAN and nitrite. All the strains reduced
TAN and nitrite level from the shrimp aquaculture wastewater
significantly (p<0.05). Strain VCM5
(GenBank accession number KJ700465) and VCM8
(GenBank accession number KJ700464) showed 99.71% sequence similarity
with the 16S rRNA gene type species Bacillus vietnamensis
15-1T (ABO99708)
and strain VCM12 (GenBank accession number KJ700463)
showed 99.05% sequence similarity with 16S rRNA gene
sequence type species Gordonia bronchialis DSM43247T (CP001802).
Keywords: Bioremediation;
shrimp; total ammonia nitrogen
ABSTRAK
Kemerosotan kualiti
air terutamanya disebabkan oleh jumlah nitrogen amonia yang tinggi
(TAN)
dan nitrit akan menjejaskan produktiviti kultur udang. Dalam kajian
ini, tiga strain asli dikenali sebagai VCM5, VCM8
dan VCM12 telah dinilai keupayaannya untuk mendegradasi TAN
dan nitrit. Strain ini telah diinokulasi ke dalam
air sisa udang akuakultur untuk meningkatkan degradasi TAN dan nitrit. Semua strain menurunkan
tahap TAN dan nitrit daripada air sisa akuakultur
udang dengan ketara (p<0.05). Strain
VCM5
(nombor penerimaan GenBank KJ700465) dan VCM8
(nombor penerimaan GenBank KJ700464) menunjukkan 99.71% persamaan
jujukan dengan spesies jenis gen 16 rRNA Bacillus vietnamensis
15-1T (ABO99708)
dan strain VCM12 (nombor penerimaan GenBank KJ700463)
menunjukkan persamaan jujukan 99.05% dengan16 rRNA spesies
jenis jujukan gen Gordonia bronchialis DSM43247
T
(CP001802).
Kata kunci: Biopemulihan; jumlah nitrogen amonia; udang
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*Pengarang
untuk surat-menyurat; email: chong@um.edu.my
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