Sains Malaysiana 44(8)(2015): 1129–1136
Supplementation of Psidium guajava (Guava) Fruit Polysaccharide
Attenuates Paracetamol-induced Liver Injury by Enhancing the Endogenous
Antioxidant Activity
(Penambahan
Polisakarida Buah Psidium guajava (Jambu Batu) Mengatenuasi
Aruhan Parasetamol daripada Kecederaan Hepar dengan Mempertingkatkan
Aktiviti Antioksida Endogenus)
ASPALILAH
ALIAS1,
FAIZAH
OTHMAN1,
ABDUL
RASHID
LI2,
AQILAH
KAMARUDDIN1,
RAFIZUL
YUSOF1
& FARIDA HUSSAN1*
1Department of Anatomy,
Faculty of Medicine, Universiti Kebangsaan Malaysia
Jalan Raja Muda Abdul
Aziz, 50300 Kuala Lumpur, Malaysia
2Forest Research Institute
Malaysia (FRIM), 52109 Kepong, Kuala Lumpur, Malaysia
Diserahkan: 27 Mei 2014/Diterima:
6 April 2015
ABSTRACT
This study was aimed
to determine the effect of polysaccharide from guava fruit on paracetamol
(PCM)-induced
liver injury. Aqueous extract of Psidium guajava fruit
was treated with 95% ethanol to collect the water soluble polysaccharide
precipitates. Thirty-eight male Sprague Dawley rats were divided
into control (C), PPG400, PCM, PPG200+PCM
and PPG400+PCM.
The control and PCM groups received 0.9% normal saline
orally while the rest were given 200 and 400 mg/kg of freeze-dried
polysaccharide (PPG) per oral for fourteen days. At day
15, the animals were orally received PCM (2 g/kg) except the control
and PPG400 groups which received 5% dimethyl sulfoxide. At day
16, the blood was collected to determine serum liver enzymes such
as transaminases (AST and ALT).
The liver tissue was harvested for determination of superoxide dismutase
(SOD),
glutathione (GSH), tumour necrosis factor-α (TNF-α),
interleukin-6(IL6), microscopic changes and glycogen content. The PCM
group showed significant higher level of AST, ALT,
TNF-α
and IL6 than those of group C. The PCM group
showed glycogen depletion, vacuolisation, loss of cell membrane,
inflammatory cells infiltration and distorted hepatocelluar cords
and narrow sinusoidal spaces. However, those PCM-induced alterations were attenuated
by the PPG supplementation. Therefore, the polysaccharide
of Psidium guajava possesses hepatoprotective activity and
can be used as a dietary supplementation for protection of liver.
Keywords: Antioxidant;
guava; liver; paracetamol; polysaccharide
ABSTRAK
Kajian ini bertujuan
untuk menentukan kesan polisakarida daripada buah jambu batu pada
parasetamol (PCM)
yang menyebabkan kecederaan hepar. 95% etanol digunakan untuk mendapatkan
ekstrak akueus daripada buah Psidium guajava untuk mengumpul
mendakan polisakarida larut air. Tiga puluh lapan ekor tikus jantan
Sprague Dawley telah dibahagikan kepada kumpulan kawalan (C), PPG400,
PCM,
PPG200+PCM
dan PPG400+PCM.
Kumpulan kawalan (C) dan PCM telah menerima 0.9% air garam secara
oral manakala yang lain telah diberikan 200 dan 400 mg/kg serbuk
polisakarida (PPG) melalui oral dalam masa empat belas hari. Pada hari
15, semua haiwan menerima PCM (2 g/kg) secara oral kecuali kumpulan
kawalan dan PPG400 menerima 5% dimetil sulfoksid.
Pada hari 16, darah diambil untuk mengetahui enzim serum hepar seperti
transaminase (AST dan ALT).
Tisu hepar telah diambil untuk mengetahui tahap superoksida dismutase
(SOD),
glutation (GSH), nekrosis tumor faktor - α (TNF -α),
interleukin -6 (IL6), perubahan mikroskopik dan kandungan
glikogen. Kumpulan PCM menunjukkan tahap signifikan yang
lebih tinggi pada AST, ALT,
TNF-α
dan IL6 berbanding dengan kumpulan C. Kumpulan PCM
menunjukkan kekurangan glikogen, pemvakuolan, kehilangan
sel membran, infiltrasi sel-sel inflamasi, perubahan pada korda
hepatosel dan ruang sinusoidal mengecil. Walau bagaimanapun, apa-apa
perubahan yang disebabkan PCM telah
dibantu oleh suplemen PPG ini. Oleh itu, polisakarida daripada
Psidium guajava mempunyai aktiviti hepatopelindung dan ia
boleh digunakan sebagai suplemen pemakanan untuk perlindungan hepar.
Kata kunci: Antioksida;
jambu batu; hepar; parasetamol; polisakarida
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*Pengarang
untuk surat-menyurat; email: khinpapah@gmail.com
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