Sains Malaysiana 45(12)(2016): 1923–1930
http://dx.doi.org/10.17576/jsm-2016-4512-17
Kesan Perencatan
Madu Gelam
terhadap Kehilangan Tulang pada Tikus
Periodontitis
(Inhibitory Effect of Gelam Honey on Bone Loss in Periodontitis Rat)
AFIFAH-RADIAH
FAUZI1*,
SALIANA
A.
AZIZ2,
NORASHIKIN
HAMZAH2,
BADIAH
BAHARIN2,
NORZANA
ABDUL
GHAFAR3
& MARIATI ABDUL RAHMAN1
1Jabatan Biologi Mulut dan
Klinikal, Fakulti
Pergigian, Universiti Kebangsaan
Malaysia
Jalan Raja Muda Abdul
Aziz, 50300 Kuala Lumpur, Wilayah Persekutuan, Malaysia
2Jabatan Periodontologi, Fakulti Pergigian, Universiti Kebangsaan
Malaysia
Jalan Raja Muda Abdul
Aziz, 50300 Kuala Lumpur, Wilayah Persekutuan, Malaysia
3Jabatan Anatomi, Fakulti Perubatan, Pusat Perubatan Universiti Kebangsaan
Malaysia
Jalan Yaacob Latif, 56000 Cheras, Kuala
Lumpur, Wilayah Persekutuan, Malaysia
Diserahkan: 26 April 2016/Diterima: 14 Oktober 2016
ABSTRAK
Periodontitis
adalah penyakit
kronik yang melibatkan kehilangan tulang dan inflamasi pada
tisu periodontium. Interleukin-6 (IL-6) dan Tumor
Necrosis Factor-α (TNF-α) merupakan
penanda pro-inflamasi
yang penting yang terlibat dalam periodontitis. Sebanyak
20 ekor tikus
Sprague-Dawley dibahagikan kepada
empat kumpulan
iaitu: Kumpulan kawalan dengan salin normal (CS);
kumpulan kawalan
dengan madu Gelam
3 g/mL (CH); kumpulan ujian periodontitis dengan salin normal (TS); dan
kumpulan ujian
periodontitis dengan madu
Gelam 3 g/mL (TH). Benang bersaiz
4/0 diikat pada
molar pertama gigi tikus
sebelah kiri
bagi tujuan rangsangan
penyakit periodontitis. Madu Gelam
diberi secara
paksa oral selama 15 hari.
Selepas
15 hari, sampel plasma dan
tisu dianalisis menggunakan kaedah Elisa dan pewarnaan histologi.
Kehilangan
tulang alveolar pada
kumpulan TS adalah
paling tinggi berbanding
dengan kumpulan kawalan, CS dan
CH
namun, tiada
perbezaan yang signifikan berbanding dengan kumpulan TH. Berdasarkan
ujian imunohistokimia,
ekspresi IL-6 dan
TNF-α
pada tisu
periodontium adalah tinggi secara
signifikan pada
kumpulan TS berbanding dengan kumpulan lain. Namun, tiada perubahan
aras IL-6
dan TNF-α yang signifikan
pada plasma ke
semua tikus kajian.
Kata kunci:
Kehilangan tulang;
madu; perantara inflamasi; periodontitis
ABSTRACT
Periodontitis is a chronic
disease which results in alveolar bone loss and inflammation at
the periodontal gingival tissue. Interleukin-6 (IL-6) and Tumor Necrosis Factor-α
(TNF-α) are the crucial pro-inflammatory
markers in periodontitis. Twenty Sprague-Dawley rats were divided
into four groups: which was control group (CS);
control treated with Gelam honey (3 g/mL) (CH);
periodontitis group (TS); and periodontitis treated with Gelam honey (3 g/mL) (TH).
The periodontitis was induced by ligating 4/0 suture on the left
first molar tooth. After 15 days, the plasma and tissue samples
were analysed using Elisa and histological
staining. Alveolar bone loss was the highest in the TS group compared
to control groups, CS and CH,
but there was no significant difference compared to TH group.
Immunohistochemistry staining showed positive expression of IL-6
and TNF-α in periodontium was significantly higher percentage
in TS group compared to TH group which was 49.27% (IL-6)
and 31.73% (TNF-α), respectively. However, there was no significant
difference in plasma level of IL-6
and TNF-α.
Keywords: Bone loss; honey; inflammatory mediators; periodontitis
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*Pengarang untuk surat-menyurat; email: afifahradiah@yahoo.com.my