Sains Malaysiana 45(12)(2016):
1931–1938
http://dx.doi.org/10.17576/jsm-2016-4512-18
Kesan Psikostres terhadap Kerosakan DNA
dan Ketaknormalan Titisan Sitoplasma Sperma Manusia
(Effect of Psychostress on DNA Damage and Cytoplasm Droplet Abnormality
in Human Sperm)
NORHAMIZAN HASHIM1,
KHAIRUL
OSMAN1*,
SITI
FATIMAH
IBRAHIM2,
ROSLIAH
HARUN3
& RAFEAH PAKRI MOHAMED2
1Program Sains Forensik, Fakulti
Sains Kesihatan, Universiti Kebangsaan Malaysia, 50300 Jalan Raja
Muda, Kuala Lumpur, Malaysia
2Jabatan Fisiologi, Fakulti Perubatan,
Universiti Kebangsaan Malaysia, Jalan Yaacob Latiff
56000 Kuala Lumpur, Wilayah Persekutuan,
Malaysia
3Bahagian Reproduksi Manusia, Lembaga
Penduduk dan Pembangunan Keluarga Negara (LPPKN)
Jalan
Raja Laut, 50350 Kuala Lumpur, Wilayah Persekutuan, Malaysia
Diserahkan:
17 Februari 2016/Diterima: 14 Oktober 2016
ABSTRAK
Ketidaksuburan idiopati dalam
kalangan lelaki telah dikaitkan dengan kesan psikostres. Walaupun
begitu, hubungan langsung antara psikostres dan ketaknormalan
kualiti semen masih samar. Maka, kajian ini dijalankan untuk menentukan
kesan psikostres terhadap kualiti semen terutama kesan berdasarkan
residu sitoplasma dan kerosakan DNA
sperma. Dalam kajian ini, responden lelaki berumur
antara 25-45 tahun dipilih secara rawak dalam kalangan pesakit
yang mendapatkan rawatan di Pusat Kesuburan Lembaga Penduduk dan
Pembangunan Keluarga Negara (LPPKN).
Seramai 331 responden akhirnya telah dipilih daripada 628 responden
selepas mengambil kira faktor penolakan. Setiap responden perlu
menjawab borang keizinan dan soal selidik GHQ-12 bagi penentuan tahap stres sebelum
pengambilan sampel semen mengikut piawaian WHO (2010).
Tahap stres diukur berdasarkan keadaan semasa responden dalam
tempoh 3-4 minggu sebelum kajian. Analisis semen, pewarnaan papanicolau
dan asai komet neutral digunakan untuk penentuan kualiti semen
dan kerosakan DNA sperma. Keputusan menunjukkan tidak terdapat
hubungan yang signifikan antara psikostres dan ketaknormalan residu
sitoplasma (U=895.50, p=0.08). Namun begitu,
psikostres memberi kesan kepada peratus morfologi normal (U=6317.50,
p<0.05) dan kerosakan DNA sperma (U=1047.00, p<0.01). Kesimpulannya,
psikostres kronik boleh menjejaskan kualiti semen dan kerosakan
DNA sperma
serta mempengaruhi kesuburan.
Kata kunci: Kerosakan DNA
sperma; psikostres; titisan sitoplasma sperma; sperma
manusia
ABSTRACT
Idiopathic infertility has
been correlated with psychostress in men. Nevertheless, direct
relationship of low semen quality to psychostress still remains
unclear. Hence, this study was conducted to evaluate the effect
of psychostress on semen quality especially on the sperms DNA and cytoplasm droplet abnormality. In this study, respondents
within 25-45 years old men were selected randomly among patients
of LPPKN.
Out of 628, only 331 respondents were selected after reviewing
research protocol exclusion criteria. Each respondent was required
to fill up a consent form and a GHQ-12 form. The latter was used to measure respondent’s
stress levels. Stress level was based on respondent’s condition
3-4 weeks prior to this study. Semen sample was collected based
on WHO (2010)
standard semen sample collection protocol. Semen analysis, papanicoulau
stain and neutral comet assay were used to measure the semen quality,
percentage of sperm cytoplasm droplet abnormality and sperm DNA damage,
respectively. The results showed that there was no significant
association between psychostress and sperm cytoplasm droplet abnormality
(U=895.50, p=0.08).
Nevertheless, the percentage of normal morphology (U=6317.50,
p<0.05) and DNA damage (U=1047.00, p<0.01) were affected by
psychostress. As a conclusion, chronic psychostress may affect
the semen quality and induced sperm DNA damage and influences the fertility
eventually.
Keywords: Human sperm; psycostress; sperm cytoplasm droplet; sperm
DNA damage
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*Pengarang untuk surat-menyurat;
email: khairos@ukm.edu.my