Sains Malaysiana 45(7)(2016): 1049–1055
Lactobacillus
plantarum G1 Microencapsulation enhanced its
Viability
during Storage and Gastrointestinal Transit
(Pemikrokapsulan Lactobacillus plantarum G1 Mempertingkatkan Kebolehhidupannya semasa
Storan dan Transit Gastrousus)
HOURIA OULED-HADDAR1,2*, MOHAMED SIFOUR1,2, TAYEB IDOUI1,3, HAMIDA BOURIDANE1 & SOMIA ARID1
1Department
of Cell and Molecular Biology, Faculty of Nature and Life Sciences, University
of Jijel,
Algeria
2Laboratory of
Molecular Toxicology, Faculty of Nature and Life Sciences, University of Jijel,
Algeria
3Laboratory of
Biotechnology, Health and environment, Faculty of Nature and Life Sciences
University of Jijel, Algeria
Diserahkan: 22 Disember 2014/Diterima: 27 Januari 2016
ABSTRACT
A Lactobacillus plantarum strain
G1 was previously isolated from chicken crop and it was found to show
interesting probiotic properties. In this study, several microencapsulation
combined materials were used to test their ability to protect cells from
gastrointestinal harsh conditions. The results on kinetics of cell release
after exposition to acidic pH (2.0) indicated that the efficiency of cell
entrapment of the gels was as follows starting from the highest capacity to the
lowest one: sodium alginate, alginate-agar (AA),
alginate-starch (AS) and κ-carrageenan. In
addition, effect of bile salts on cell release was also tested; the results
showed that the efficiency of entrapment of the gels was different and as
follows starting from the highest capacity to the lowest one: AA,
sodium alginate, κ-carrageenan, and AS. Moreover, viability of free
and alginate-microencapsulated Lactobacillus plantarum after exposure to
acid, bile and pancreatic enzymes was investigated; the results showed that
microencapsulation was capable of protecting cells against the harsh conditions
of the gastrointestinal tract. In addition, microencapsulation enhanced the
viability of Lb. plantarum during cold storage for 2 weeks and during a
range of heat treatments.
Keywords: Gastrointestinal conditions; Lactobacillus plantarum; microencapsulation;
probiotics
ABSTRAK
Strain G1 Lactobacillus
plantarum diasingkan daripada tembolok ayam dan ia didapati menunjukkan sifat probiotik yang menarik. Dalam
kajian ini, beberapa bahan gabungan pemikrokapsulan telah digunakan untuk
menguji keupayaan mereka untuk melindungi sel daripada keadaan yang teruk dalam
gastrousus. Keputusan pembebasan sel kinetik selepas pendedahan kepada
pH berasid (2.0) menunjukkan bahawa kecekapan pemerangkapan sel oleh gel adalah
seperti berikut; bermula daripada kapasiti tertinggi ke terendah: natrium
alginat, alginat-agar (AA), alginat-kanji (AS)
dan κ-carrageenan. Di samping itu, kesan garam hempedu ke atas pembebasan
sel juga telah diuji; keputusan menunjukkan bahawa kecekapan pemerangkapan gel
adalah berbeza dan seperti berikut bermula daripada kapasiti tertinggi ke
terendah: AA, natrium alginat, κ-carrageenan dan AS.
Selain itu, kebolehhidupan Lactobacillus plantarum yang bebas dan
alginat-pemikrokapsulan selepas terdedah kepada asid, hempedu dan enzim
pankreas dikaji; hasil kajian menunjukkan bahawa pemikrokapsulan mampu
melindungi sel terhadap keadaan yang teruk dalam saluran gastrousus. Di samping itu, pemikrokapsulan meningkatkan kebolehhidupan Lb.
plantarum semasa penyimpanan sejuk selama 2 minggu dan semasa pelbagai
rawatan haba.
Kata kunci: Keadaan gastrousus; Lactobacillus plantarum; pemikrokapsulan; probiotik
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*Pengarang
untuk surat-menyurat; email: hrourou2002@gmail.com
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