Sains Malaysiana 45(7)(2016): 1131–1137
Dietary UKMR-1 Roselle Supplementation
Prevents Nicotine-Induced Cardiac Injury by Inhibiting Myocardial
Oxidative Stress
(Suplemen Rosel Diet UKMR-1 Mencegah Kecederaan Jantung Aruhan Nikotin dengan Menghalang Tekanan Oksidatif Miokardium)
ANAND RAMALINGAM,
SITI
BALKIS
BUDIN,
YI-CHENG
LIM,
YIANG-NEE
LISLIVIA
SI
& SATIRAH
ZAINALABIDIN*
Programme of Biomedical Science, School of Diagnostic
Science and Applied Health,
Faculty
of Health Sciences, Universiti Kebangsaan
Malaysia
Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Federal Territory, Malaysia
Diserahkan: 22 Oktober
2015/Diterima: 9 Februari
2016
ABSTRACT
UKMR-1,
a local variant of mutant Roselle strain (Hibiscus sabdariffa)
is enriched with free radical scavenging polyphenols such as anthocyanin,
vitamin C and hydroxycitric acid. However,
pharmacological actions of UKMR-1 are not fully known. This study
was conducted to determine whether supplementation of aqueous UKMR-1
calyx extract was able to protect against nicotine-induced cardiac
injury in rats. In this experimental study, healthy male albino
rats were randomly allotted into three groups (n=7 per group):
control, nicotine and UKMR-1+Nicotine
groups. Nicotine (0.6 mg/kg, i.p.) was
administered to both nicotine and UKMR-1+Nicotine groups for 28 consecutive
days. UKMR-1+Nicotine group also received 100
mg/kg UKMR-1 extract orally via gavage 30 min prior to nicotine
injection, daily. UKMR-1+Nicotine group had significantly
(p<0.05) higher lactate dehydrogenase (LDH)
activity, as well as lower malondialdehyde
content in heart tissue homogenate than nicotine group, suggesting
its cardio protective activity by inhibition of lipid peroxidation.
UKMR-1
also lowered (p<0.05) the blood pressure in nicotine-administered
rats. In addition, UKMR-1 significantly (p<0.05)
restored activities of cytosolic superoxide dismutase, glutathione
peroxidase and glutathione-S-transferase as well as redox balance
ratio (GSH:GSSG). In conclusion, UKMR-1
was able to protect against myocardial injury in rat model of nicotine
administration possibly by inhibiting oxidative stress.
Keywords: Antioxidant; antihypertensive;
cardioprotective; Hibiscus
sabdariffa; nicotine
ABSTRAK
UKMR-1
yang merupakan salah
satu varian mutan
Rosel tempatan
(Hibiscus
sabdariffa) yang diperkaya dengan
polifenol perangkap
radikal bebas seperti
antosianin, vitamin C dan
asid hidrosisitrik. Walau bagaimanapun, tindakan farmakologi UKMR-1 belum
diketahui sepenuhnya.
Kajian ini dijalankan untuk menentukan sama
ada suplementasi
ekstrak akues UKMR-1
dapat melindungi
tikus daripada kecederaan jantung aruhan nikotin. Dalam kajian eksperimental
ini, tikus jantan Albino yang sihat dibahagikan secara rawak kepada tiga
kumpulan (n=7 setiap
kumpulan): kawalan,
nikotin dan UKMR-1
+ nikotin. Nikotin (0.6 mg/kg, ip) telah
diberi kepada
kedua-dua kumpulan nikotin dan UKMR-1
+ nikotin selama
28 hari berturut-turut. Kumpulan UKMR-1
+ nikotin turut
menerima ekstrak 100 mg/kg UKMR-1
secara oral melalui
gavage 30 minit sebelum
suntikan nikotin pada setiap hari. Kumpulan UKMR-1+nikotin mempunyai
aktiviti laktat
dehidrogenase (LDH) yang lebih
tinggi secara
signifikan (p<0.05)
serta kandungan
malondialdehid yang lebih rendah dalam homogent
tisu jantung
daripada kumpulan nikotin, mencadangkan bahawa aktiviti perlindungan jantung dengan mencegah peroksidaan lipid. UKMR-1
juga menurunkan (p<0.05)
tekanan darah
dalam tikus diadministrasi
nikotin. Di samping
itu, UKMR-1 memulihkan
aktiviti superoksida
dismutase sitosolik, glutation
peroksida dan glutation-S-transferase
serta nisbah
keseimbangan redoks (GSH:
GSSG) dengan signifikan
(p<0.05). Secara kesimpulannya, UKMR-1
dapat memberi
perlindungan terhadap kecederaan jantung dalam model tikus diadministrasi nikotin, berkemungkinan melalui perencatan tekanan oksidatif.
Kata kunci: Antioksidan;
antihipertensi; Hibiscus sabdariffa; nikotin; perlindungan jantung
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*Pengarang untuk
surat-menyurat; email: satirah@ukm.edu.my
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