Sains Malaysiana 46(10)(2017): 1721–1726
http://dx.doi.org/10.17576/jsm-2017-4610-07
The
Effects of Malaysian Herb, Labisia
pumila var. alata on Oestrous Cyclicity and Reproductive Parameters of Nulliparous Rats
(Kesan Herba Malaysia, Labisia pumila var.
alata
ke atas Kitaran
Oestrus dan
Parameter Reproduktif Tikus Nuliparus)
WAN EZUMI
MOHD
FUAD1*,
HASNAN
JAAFAR2
& SITI AMRAH SULAIMAN3
1Programme of Biomedicine,
School of Health Science, Universiti Sains Malaysia Health Campus, 16150 Kubang
Kerian, Kelantan Darul Naim,
Malaysia
2Department of Pathology,
School of Medical Sciences, Universiti
Sains Malaysia Health Campus, 16150 Kubang
Kerian, Kelantan Darul Naim,
Malaysia
3Department of Pharmacology,
School of Medical Sciences, Universiti
Sains Malaysia Health Campus, 16150 Kubang
Kerian, Kelantan Darul Naim,
Malaysia
Diserahkan: 2 Mei 2016/Diterima: 20 Februari 2017
ABSTRACT
Numerous nutraceutical products
containing the powdered or extracted parts of Labisia pumila (Myrsinaceae) have been widely available for years in Malaysia,
aimed at women of reproductive age. However, there is scarce of
information concerning the effects of this plant on the reproductive
function of nulliparous females prior to the present study. The
toxicity potential of Labisia pumila var. alata (LPA)
on oestrous cycle and reproductive parameters
was evaluated in groups of 40 virgin rats. They were administered
with LPA
at the doses of 0 (control), 20, 200 or 1000 mg/kg/day
for duration of three weeks. The results obtained indicated that
the administration of LPA
at all dose levels did not cause mortality nor show
noticeably any treatment-related signs of toxicity on the physical
appearance, behaviour and body weight of all the rats studied. The pattern
and length of oestrous cyclicity
as well as the changes in reproductive hormones were statistically
comparable among groups. No indications of abnormalities in the
histology of uterus and vagina were observed. However, the presence
of ovarian follicular cysts has raised apprehension that requires
further investigation. The current findings suggested that oral
treatment of LPA were associated with toxicity
concerns
Keywords: Labisia pumila var.
alata;
nulliparous; oestrous cycle; ovarian cyst;
rat
ABSTRAK
Pelbagai produk nutraseutik yang mengandungi serbuk atau ekstrak Labisia pumila (Myrsinaceae) terdapat
secara meluas di Malaysia sejak sekian lama, disasarkan kepada wanita dalam usia reproduktif. Walau bagaimanapun, terdapat sedikit maklumat mengenai kesan tumbuhan ini ke
atas fungsi
reproduktif betina nuliparus sebelum kajian ini dijalankan.
Potensi
ketoksikan Labisia pumila var. alata
(LPA)
telah dinilai
ke atas kitaran
oestrus dan
parameter reproduktif bagi sekumpulan 40 tikus dara. Tikus tersebut diberikan
LPA
pada dos 0 (kawalan),
20, 200 or 1000 mg/kg/hari selama tiga
minggu. Keputusan yang diperoleh menunjukkan
bahawa pemberian
dos LPA pada semua peringkat
tidak menyebabkan
kematian, juga tidak menunjukkan sebarang tanda ketoksikan yang ketara pada keadaan
fizikal, perlakuan
dan berat badan
semua tikus
yang dikaji. Corak dan tempoh kitaran oestrus serta perubahan hormon reproduktif adalah setanding secara statistik dalam kalangan semua kumpulan rawatan. Tiada indikasi ketaknormalan
yang diperhatikan dalam
histologi uterus dan vagina.
Walau
bagaimanapun kehadiran sista folikel ovari
telah menimbulkan
kebimbangan yang memerlukan kajian lanjut. Penemuan dalam kajian ini
menunjukkan bahawa
rawatan oral LPA dikaitkan
dengan kebimbangan
kesan toksik.
Kata kunci: Kitar
oestrus; Labisia pumila
var. alata;
nuliparus; sista
ovari; tikus
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*Pengarang untuk surat-menyurat; email: wanezumi@usm.my
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