Sains Malaysiana 46(12)(2017): 2273–2280
http://dx.doi.org/10.17576/jsm-2017-4612-03
Ovarian
Maturation Stages of Wild and Captive Mud Crab, Scylla olivacea
Fed with Two Diets
(Peringkat
Kematangan Ovari bagi Ketam Lumpur Liar dan Kurungan Scylla olivacea
Diberi Makan dengan Dua Diet)
AZMIE GHAZALI1,
NOORDIYANA
MAT
NOORDIN2,
AMBOK
BOLONG
ABOL-MUNAFI1,
MOHAMAD N.
AZRA1
& MHD IKHWANUDDIN1
1Institute of Tropical
Aquaculture, Universiti Malaysia Terengganu, 21030 Kuala Terengganu,
Terengganu Darul Iman, Malaysia
2School of Fisheries
and Aquaculture Sciences, Universiti Malaysia Terengganu
21030 Kuala Terengganu,
Terengganu Darul Iman, Malaysia
Diserahkan: 25
Julai 2016/Diterima: 25 Mei 2017
ABSTRACT
This study was aimed to determine
the ovarian maturation stages of wild and captive orange mud crab,
Scylla
olivacea fed with different diets via gonadosomatic status, oocyte
diameter and histological examinations. Captive crabs were fed with
blood cockle, Anadara granosa, or fish, Decapterus spp.
Through the histological examinations, ovarian maturation stages
of wild and captive S. olivacea was classified into four
stages: Immature (Stage 1), Early maturing (Stage 2), Pre-maturing
(Stage 3) and Fully matured (Stage 4). Gonadosomatic Index of wild
and captive crabs remained low during immature and 2 but increased
significantly (p<0.05) in pre-maturing and 4 ovaries.
Oocytes size were significantly different (p<0.05) in
all ovarian maturation stages of wild and captive crabs. Follicle
cells surround the oocyte of immature ovary and small yolk globules
start to appear in early maturing ovary with large nucleus size.
Oocyte size increased significantly (p<0.05) and yolk
globule obviously appeared in pre-maturing ovary. Large and fused
yolk globules appeared in the oocytes of fully matured ovary with
nucleus was barely visible. The present study revealed that, ovarian
maturation stages of S. olivacea are closely related to its
morphological appearance and cellular development.
Keywords: Aquatic science;
fisheries; invertebrate; nutrition; reproductive biology
ABSTRAK
Kajian ini bertujuan untuk
menentukan tahap pematangan ovari bagi ketah lumpur liar dan kurangan
jenis Scylla
olivacea yang diberi makan dua diet yang berlainan dengan melalui
status gonadosomatik, diameter oosit dan pemeriksaan histologi.
Ketah lumpur telah diberi makanan seperti kerang darah seperti Anadara
granosa, atau ikan seperti Decapterus spp. Peringkat pematangan
ovari bagi S. olivacea liar dan kurungan telah dibahagi kepada
empat peringkat melalui pemeriksaan histologi: Tidak matang (Tahap
1), Matang awal (Tahap 2), Pra-matang (Tahap 3) dan Matang sepenuh
(Tahap 4). Indeks Gonadosomatik ketam lumpur liar dan kurungan adalah
rendah pada tahap tidak matang dan 2 ovari tetapi meningkat dengan
ketara (p<0.05) bagi pra-matang dan 4 ovari. Saiz oosit
adalah berbeza dengan ketara (p<0.05) bagi semua peringkat
pematangan ovari bagi ketam lumpur liar dan kurangan. Sel-sel folikel
mengelilingi oosit ovuri yang tidak matang dan kuning telur yang
kecil mula berlaku pada tahap matang awal ovari dengan saiz nukleus
besar. Saiz oosit meningkat secara ketara (p<0.05) dan
globul telur kuning jelas kelihatan pada tahap pra-matang ovari.
Kata kunci: Biologi pembakan; invertebrat; nutrisi; perikanan; sains
akuatik
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*Pengarang untuk surat-menyurat; email:
ikhwanuddin@umt.edu.my
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