Sains Malaysiana 46(12)(2017): 2447–2454
http://dx.doi.org/10.17576/jsm-2017-4612-21
A
Novel Silver Nanoparticles-based Sensing Probe for the Detection
of Japanese Encephalitis Virus Antigen
(Penderia Baru yang Berasaskan Nano
zarah Perak untuk
Pengesanan Antigen Virus Ensefalitis
Jepun)
LIH SHAN
LIM,
SUK
FUN
CHIN*,
SUH
CEM
PANG,
MAGDLINE
SIA
HENRY
SUM
& DAVID PERERA
Department
of Chemistry, Faculty of Resource Science and Technology, Universiti
Malaysia Sarawak
94300
Kota Samarahan, Sarawak Bumi
Kenyalang, Malaysia
Diserahkan: 1 September 2016/Diterima: 3 April 2017
ABSTRACT
A novel silver nanoparticles
(Ag NPs)-based optical sensing probe has been developed for
the detection of Japanese Encephalitis virus (JEV).
Ag NPs were initially deposited onto amine functionalized glass
slides. Subsequently, JEV antibodies were self-assembled
onto surfaces of Ag NPs to form optical sensing probes.
The detection of JEV antigen was observed via changes
in light absorbance by Ag NPs upon occurrence of JEV antigen-antibody
bindings. A highly sensitive and rapid optical sensing probe
for JEV
antigen with a detection limit of 12.8 ng/mL (for
S/N ratio = 3) and an analysis assay time of 1 h had been demonstrated.
Keywords: Antibody; biosensor;
Japanese Encephalitis Virus (JEV) antigen; optical sensing
probe; silver nanoparticles (Ag NPs)
ABSTRAK
Biopenderia optik baru yang berasaskan
nano zarah
perak (Ag NPs) telah
dihasilkan untuk
pengesanan antigen virus Ensefalitis
Jepun (JEV). Ag NPs telah dimendapkan di atas slaid kaca yang difungsikan dengan amino.
Seterusnya antibodi JEV dipegunkan
pada permukaan
Ag NPs
untuk membentuk
biopenderia optik. Pengesanan terhadap JEV antigen diperhatikan
melalui perubahan
penyerapan cahaya oleh Ag NPs apabila
berlakunya pengikatan
antara JEV antigen dan
antibodi. Satu
biopenderia optik yang sensitif bagi pengesanan
JEV
dengan had pengesanan
(LOD)
sebanyak 12.8 ng/mL (ratio S/N = 3)
dan 1 jam masa analisis telah dihasilkan.
Kata kunci: Antibodi;
antigen virus Ensefalitis Jepun
(JEV);
biopenderia; nano
zarah perak (Ag NPs);
perangsang optic
RUJUKAN
Agnihotri, S., Mukherji, S. & Mukherji, S.
2013. Immobilized silver nanoparticles enhance contact killing
and show highest efficacy: Elucidation of the mechanism of bactericidal
action of silver. Nanoscale 5: 7328-7340.
Baldassarre,
M., Li, C., Eremina, N., Goormaghtigh,
E. & Barth, A. 2015. Simultaneous fitting of absorption
spectra and their second derivatives for an improved analysis
of protein infrared spectra. Molecules 20: 12599-12622.
Campbell, G.L.,
Hills, S.L., Fischer, M., Jacobson, J.A., Hoke, C.H., Hombach,
J.M., Marfin, A.A., Solomon, T., Tsai,
T.F., Tsu, V.D. & Ginsburg, A.S. 2011. Estimated global incidence
of Japanese encephalitis: A systematic review. BMC Medicine
89: 766-774.
Cardosa,
M.J., Wang, S.M., Sum, M.S.H. & Tio,
P.H. 2002. Antibodies against
prM protein distinguish between previous
infection with dengue and Japanese encephalitis viruses. BMC
Microbiology 2: 9.
Chen, Z., Liao,
Y., Ke, X., Zhou, J., Chen, Y., Gao,
L., Chen Q. & Yu, S. 2011. Comparison of reverse transcription
loop-mediated isothermal amplification, conventional PCR and
real-time PCR assays for Japanese encephalitis virus. Molecular
Biology Reports 38: 4063-4070.
Chin, S.F., Iyer, K.S. & Raston, C.L. 2009. Facile and green approach to fabricate gold and
silver coated superparamagnetic nanoparticles. Crystal Growth
and Design 9: 2685-2689.
El-Sayed,
I.H., Huang, X. & El-Sayed, M.A. 2005. Surface plasmon
resonance scattering and absorption of anti-EGFR antibody conjugated
gold nanoparticles in cancer diagnostics: Applications in oral
cancer. Nano Letters 5: 829-834.
Endo,
T., Yamamura, S., Nagatani, N., Morita,
Y., Takamura, Y. & Tamiya, E. 2005. Localized surface
plasmon resonance based optical biosensor
using surface modified nanoparticle layer for label-free monitoring
of antigen-antibody reaction. Advance Material 6(5):
491-500.
Frederix,
F., Friedt, J., Choi, K., Laureyn,
W., Campitelli, A., Mondelaers,
D., Maes, G. & Borghs,
G. 2003.
Biosensing based on light absorption
of nanoscaled gold and silver particles. Analytical Chemistry
7: 6894-6900.
Hrapovic,
S., Liu, Y., Enright, G., Bensebba,
F. & Luong, J.H.T. 2003.
New strategy for preparing thin gold films on modified glass
surfaces by electrodeless deposition. Langmuir 19: 3958-3965.
Huy,
T.Q., Hanh, N.T.H., Thuy, N.T., Chung, P.V., Nga, P.T. &
Tuan, M.A. 2011. A novel biosensor
based on serum antibody immobilization for rapid detection of
viral antigens. Talanta
86: 271-277.
Jensen,
T.R., Malinsky, M.D., Haynes, C.L. & Duyne, R.P.V. 2000. Nanosphere lithography: Tunable localized surface plasmon resonance spectra of silver nanoparticles. Journal
of Physical Chemistry: B 104: 10549-10556.
Kumar,
A., Kumar, R. & Kaur, J. 2012. Japanese Encephalitis: Medical emergency
in India. Asian Journal of Pharmaceutical and Clinical Research
5: 9-12.
Labib,
M., Martić, S., Shipman, P.O.
& Kraatz, H. 2011. Electrochemical analysis of HIV-1 reverse
transcriptase serum level: Exploiting protein binding to a functionalized
nanostructured surface. Talanta
85: 770-778.
Lee,
H.J., Min, K.I., Park, K.H., Choi, H.J., Kim, M.K., Ahn,
C.Y., Hong, Y.J. & Kim, Y.B. 2014. Comparison of JEV neutralization
assay using pseudotyped JEV with the
conventional plaque-reduction neutralization test. Journal
of Microbiology 52: 435-440.
Li,
F., Mei, L., Li, Y., Zhao, K., Chen, H., Wu, P., Hua, Y. &
Cao, S. 2011. Facile fabrication
of magnetic gold electrode for magnetic beads-based electrochemical
immunoassay: Application to the diagnosis of Japanese encephalitis
virus. Biosensors and Bioelectronic
26: 4253-4256.
Oya,
A. & Kurane, I. 2007. Japanese Encephalitis
for a reference to international travelers. Journal of Travel
Medicine 14: 259-268.
Pillai,
Z.S. & Kamat, P.V. 2004. What factors control
the size and shape of silver nanoparticles in the citrate ion
reduction method? Journal of Physical Chemistry: B 108:
945-951.
Rashid,
M.U., Bhuiyan, M.K.H. & Quayum,
M.E. 2013.
Synthesis of silver nano
particles (Ag-NPs) and their uses for quantitative analysis
of vitamin C tablets. Journal of Pharmaceutical Sciences
12: 29-33.
Riboh,
J.C., Haes, A.J., McFarland, A.D.,
Yonzon, C.R. & Duyne, R.P.V. 2003. A nanoscale optical
biosensor: Real-time immunoassay in physiological buffer enabled
by improved nanoparticle adhesion. The Journal of Physical
Chemistry 107: 1772-1780.
Sharma,
A., Matharu, Z., Sumana,
G., Solanki, P.R., Kim, C.G. & Malhotra, B.D. 2010. Antibody immobilized
cysteamine functionalized-gold nanoparticles
for aflatoxin detection. Thin Solid Films 519: 1213-1218.
Sharma,
P., Kumar, B. & Suri, C.R. 2012. Antibody functionalized gold nanoprobes based colorimetric assay for the direct detection
of phenylurea herbicide. Environment
and Pollution 1: 12-19.
Sirivichayakul, C., Sabchareon, A., Limkittikul, K.
& Yoksan, S. 2014. Plaque reduction
neutralization antibody test does not accurately predict protection
against dengue infection in Ratchaburi
cohort, Thailand. Virology Journal 11: 48.
Solomon, T. 1997.
Viral encephalitis in Southeast Asia.
Neurological Infections and Epidemiology 2: 191-199.
Solomon, T., Kneen, R., Dung, N.M., Khanh, V.C.
& Thuy, T.T. 1998. Poliomyelitis-like
illness due to Japanese encephalitis virus. Lancet
351: 1094-1097.
Swami,
R., Ratho, R.K., Mishra, B. &
Singh, M.P. 2008. Usefulness of RT-PCR for the diagnosis of Japanese encephalitis in
clinical samples. Scandinavian Journal of Infectious
Diseases 40: 815-820.
Szymanski,
M.S. & Porter, R.A. 2013. Preparation and quality control of silver
nanoparticle-antibody conjugate for use in electrochemical immunoassays.
Journal of Immunological Methods 387: 262-269.
Tam,
P.D., Tuan, M.A., Huy, T.Q., Le, A.
& Hieu, N.V. 2010. Facile preparation of
a DNA sensor for rapid herpes virus detection. Materials
Science and Engineering: C 30: 1145- 1150.
Tran,
Q.H., Hanh, N.T.H., Chung, P.V., Anh, D.D., Nga, P.T. &
Tuan, M.A. 2011. Characterization
of immobilization methods of antiviral antibodies in serum for
electrochemical biosensors. Applied
Surface Science 257: 7090-7095.
Tran, Q.H., Nguyen,
T.H.H., Mai, A.T., Nguyen, T.T., Vu, Q.K. & Phan, T.N. 2012. Development of electrochemical
immunosensors based on different serum antibody immobilization
methods for detection of Japanese encephalitis virus. Advances
in Natural Science Nanoscience and Nanotechnology 3: 1-6.
Zhao, J., Zhang, X., Yonzon, C.R., Haes, A.J. & Duyne,
R.P.V. 2006. Localized surface plasmon
resonance biosensors. Nanomedicine 1: 219-228.
*Pengarang
untuk surat-menyurat;
email: sukfunchin@gmail.com