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Sains Malaysiana 46(12)(2017): 2497–2506 http://dx.doi.org/10.17576/jsm-2017-4612-27 Characterization of Aerobic Granular Sludge
Developed under Variable and Low Organic Loading Rate (Pencirian Enap Cemar Berbutir
Aerobik Dibangunkan
di bawah Pemboleh Ubah dan Kadar Pemuatan Organik Rendah) NIK AZIMATOLAKMA
AWANG1,
MD
GHAZALY
SHAABAN1*,
LEE
CHOON
WENG2
& BONG
CHUI
WEI2 1Department of Civil
Engineering, Faculty of Engineering, University of Malaya, 50603
Kuala Lumpur, Federal Territory, Malaysia 2Institute of Biological
Sciences, Faculty of Science, University of Malaya, 50603 Kuala
Lumpur, Federal Territory, Malaysia Diserahkan: 14 Januari 2017/Diterima: 14 Mei 2017 ABSTRACT Understanding the formation
of aerobic granules sludge (AGS) under the variations of organic
loading rate (OLR) could give a different insight on
AGS
stability, which had become the bottleneck for
practical application in sewage treatment. This study demonstrates
the formation of AGS
that had previously been stored for eight months
at 5ºC in sequencing batch reactor (SBR)
with sewage as substrate. Despite being redeveloped under variable
OLR
of 0.26 to 0.81 kg CODs/m3 d
and low superficial air velocity (SAV) of 1.33 cm/s, the loose structure
of AGS during storage can be recovered within 46 days of formation
process. Variations in OLR intrude the formation process
particularly during low OLR, resulting in longer period
to achieve mature AGS or full granulation of biomass in
reactor. The next-generation sequencing (NGS) analysis indicated that the
shift in microbial community from Rhodocyclaceae
to Comamonadaceae class for denitrification
process was accommodated with the changes in the AGS size from 326 μm to more than 600 μm.
Keywords: Aerobic granular
sludge; next generation sequencing; sewage; stability ABSTRAK Memahami pembentukan enap cemar berbutir aerobik (AGS) di bawah
variasi kadar
pembebanan organik
(OLR)
boleh memberikan
pandangan yang berbeza pada kestabilan AGS,
yang menjadi masalah
utama untuk aplikasi
praktik dalam
rawatan kumbahan. Kajian ini menunjukkan
pembentukan semula
AGS
yang sebelum ini
telah disimpan selama 8 bulan pada 5ºC, dalam reaktor penjujukan berkumpulan (SBR) dengan
kumbahan sebagai
substrat. Walaupun dibangunkan semula di bawah pemboleh ubah OLR daripada
0.26 kepada 0.81 CODs
kg/m3 d dan
halaju udara
cetek rendah (SAV)
1.33 cm/s, struktur longgar
AGS
semasa penyimpanan
dapat dipulihkan
dalam tempoh 46 hari daripada proses pembentukan semula. Variasi dalam OLR mengganggu
proses pembentukan terutamanya
semasa OLR rendah
dan menyebabkan
tempoh yang lebih lama untuk mencapai AGS matang atau pembutiran
penuh biojisim
dalam reaktor. Analisis penjujukan generasi akan
datang (NGS) menunjukkan
pertukaran komuniti
mikrob daripada kelas Rhodocyclaceae kepada Comamonadaceae untuk proses denitrifikasi adalah disebabkan oleh perubahan dalam saiz AGS daripada 326 μm kepada lebih 600 μm. Kata kunci:
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