Sains Malaysiana 50(4)(2021): 897-905

http://doi.org/10.17576/jsm-2021-5004-02

Anatomi dan Mikromorfologi Tetrastigma rafflesiae (Vitaceae)

(Anatomy and Micromorphology of Tetrastigma rafflesiae (Vitaceae))

SYAMSURINA ARSHAD, MOHD AFIQ AIZAT JUHARI*, NORAINI TALIP, NOR AZILAH ABDUL WAHAB & JUMAAT ADAM

Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43650 UKM Bangi, Selangor Darul Ehsan, Malaysia

Diserahkan: 18 September 2018/Diterima: 2 September 2020

ABSTRAK

Kajian anatomi dan mikromorfologi telah dilakukan ke atas Tetrastigma rafflesiae (Miq.) Planchon, perumah kepada bunga pakma di Perak, Pahang dan Kelantan. Penglibatan kajian ini merangkumi anatomi keseluruhan tumbuhan ini, iaitu bahagian daun, batang, sulur paut dan akar kerana kajian anatomi masih dangkal di Malaysia amnya dan dunia khasnya. Sampel tumbuhan ini diperoleh dari Sungai Bongor, Grik, Perak, Sungai Yol, Raub, Pahang dan Lojing, Kelantan. Teknik piawai dalam anatomi tumbuhan digunakan dan ini melibatkan penggunaan mikroskop cahaya dan mikroskrop imbasan elektron (SEM). Hasil kajian anatomi menunjukkanT. rafflesiae mempunyai corak bekas vaskular dengan sistem tertutup pada petiol, tulang daun, batang dan akar; hablur didapati hadir pada petiol, tulang daun, lamina, peruratan, batang dan akar dan dinding antiklin abaksial dan adaksial adalah daripada lurus ke melengkung. Hasil mikromorfologi memperlihatkan spesies ini mempunyai stomata homostomatik dan hipostomatik daripada jenis diasitik. Lilin epikutikel berbentuk lembaran dan lapisan filem pada bahagian adaksial manakala berbentuk granul, lapisan filem dan lembaran pada bahagian abaksial. Hasil kajian ini dapat digunakan sebagai perintis kepada kajian anatomi spesiesTetrastigma yang lain.

Kata kunci: Anatomi; mikromorfologi; Tetrastigma rafflesiae

ABSTRACT

Anatomy and micromorphology study were undertaken on Tetrastigma rafflesiae (Miq.) Planchon, the host of Rafflesia flower in Peninsular Malaysia. The assessment included all the anatomy parts of this plant (leaves, stems, tendrils and roots) because the anatomy study of this plant still lacking in Malaysia and also the entire world. The plant samples were collected from Sungai Bongor, Grik, Perak, Sungai Yol, Raub, Pahang and Lojing, Kelantan. Standard techniques in plant anatomy were employed, including analyses under light microscopy and scanning electron microscopy (SEM). Anatomy results showed that T. rafflesiae has close vascular bundle system at petiole, midrib, stems and root; druses are present at petioles, midribs, laminas, veins, stem and roots; abaxial and adaxsial anticlinal walls are straight to curve. Micromorphology results showed that this species has homostomatic and hypostomatic stomata of diacytic type. Epicuticular wax is in layers and films at adaxial, and in granule, films and layers at abaxial. This study can be used as a pioneer for the anatomy investigation of other Tetrastigma species.

Keywords: Anatomy, micromorphology, Tetrastigma rafflesiae

RUJUKAN

Barthlott, W., Neinhuis, C., Cutler, D., Ditsch, F., Meusel, I., Theisen, I. & Wilhelmi, H. 1998. Classification and terminology of plant epicuticular waxes. Botanical Journal of the Linnean Society 126(3): 237-260.

Cutler, D.F. 1978. Applied Plant Anatomy. New York: Longman Group Limited.

da Silva, N.R., Florindo, J.B., Gómez, M.C., Rossatto, D.R., Kolb, R.M. & Bruno, O.M. 2015. Plant identification based on leaf midrib cross-section images using fractal descriptors. PLoS ONE 10(6): 1-14.

Fahn, A. 1967. Plant Anatomy. London: Pergamon Press Ltd.

Febriyanti, E., Suwirmen & Idris, M. 2013. Induksi perakaran tunas Tetrastigma rafflesiae Miq. pada media Murashige-skoog dengan penambahan beberapa konsentrasi indole-3-butyric acid (IBA) secara in vitro. Jurnal Biologi Universitas Andalas 2(2): 161-167.

Gerbode, S.J., Puzey, J.R., McCormick, A.G. & Mahadevan, L. 2012. How the cucumber tendril coils and overwinds? Science 337(6098): 1087-1091.

Huang, L., Shi, X., Wang, W., Ryu, K.H. & Schiefelbein, J. 2017. Diversification of root hair development genes in vaskular plants. Plant Physiology 174(3): 1697-1712.

Jamili, N. 2004. Rafflesia Bunga Terbesar di Dunia. Kota Kinabalu: Natural History Publication.

Jamili, N. 2001. Rafflesia of the World. Kota Kinabalu: Penerbit Sabah Parks.

Johansen, D.A. 1940. Plant Microtechnique. New York: McGraw-Hill Book Company Inc.

Jumaat, H.A., Mohd Afiq Aizat, J., Rahmah, M., Nor Azilah, A.W., Syamsurina A., Mohd Paiz, K., Mohd Firdaus, M.R. & Kiew-Lian, W. 2016. Rafflesia tuanku-halimii (Rafflesiaceae): A new species from Peninsular Malaysia. Sains Malaysiana 45(11): 1589-1595.

Jumaat, H.A., Rahmah, M., Mohd Afiq Aizat, J., Nik Nadira Farhana, N.A. & Kiew-Lian, W. 2013. Rafflesia sharifah-hapsahiae (Rafflesiaceae), a new species from Peninsular Malaysia. Turkish Journal of Botany 37(6): 1038-1044.

Kamarudin, M.S. 2007. Rafflesia: Magnificient Flower of Sabah. Kota Kinabalu: Natural History Publications (Borneo).

Konlechner, C. & Sauer, U. 2016. Ultrastructural leaf features of grapevine cultivars. (Vitis vinifera L. ssp. vinifera). OENO One 50(4): 195-207.

Krishna, T.P.A., Krishna, T.P.A., Chithra, N.D., Deepa, P.E., Darsana, U., Sreelekha, K.P., Juliet, S., Nair, S.N., Ravindran, R., Kumar, K.G.A. & Ghosh, S. 2014. Acaricidal activity of petroleum ether extract of leaves of Tetrastigma leucostaphylum (Dennst.) Alston against Rhipicephalus (Boophilus) annulatus. The Scientific World Journal 2014: 1-6.

Latiff, A. 2015. Studies in Malesian Vitaceae XV. Revision of Tetrastigma (Miq.) Planch. Sect. Carinata Latiff. Malayan Nature Journal 67(3): 318-327.

Latiff, A. 1983. Studies in Malesian Vitaceae VII. The genus Tetrastigma in the Malay Peninsula. Gardens Bulletin of Singapore 36(2): 213-228.

Latiff, A. & Wong, M. 2003. A new species of Rafflesia from Peninsular Malaysia. Folia Malaysiana 4: 135-146.

Lux, A. & Rost, T.L. 2012. Plant root research: The past, the present and the future. Annals of Botany 110(2): 201-204.

Martin, J.T. & Juniper, B.E. 1970. The Cuticles of Plants. Edinburgh: Edward Arnold (Publishers) Ltd.

Muhammad, A.Z., Nor, S.O., Zaini, Y., Mohd Lias, K. & Jamil, T. 2014. Identification of alkaloid compound and antioxidant activity of Rafflesia cantleyiand its host, Tetrastigma tuberculatum. The Open Conference Proceedings Journal 5: 18-20.

Najmaddin, C., Hussin, K. & Maideen, H. 2013. Comparative leaf anatomy of selected species in Vitaceae and Leeaceae. American Journal of Applied Sciences 10(4): 414-417.

Narasimhan, S. 2015. Morphometric characters determine tendril biomechanics of Luffa acutangulavar. acutangula. In 4th World Conference on Applied Sciences, Engineering & Technology. Anjuran Kumamoto University, Jepun.

Nasihah, M., Zulhazman, H., Siti Munirah, M.Y., Wan Norqayyum Nadia, W.A. & Latiff, A. 2016. Tetrastigma hookeri(Laws.) Planch. (Vitaceae), a host plant for Rafflesia kerriiMeijer in Peninsular Malaysia. Malayan Nature Journal 68(1&2): 33-39.

Onoda, Y., Schieving, F. & Anten, N.P.R. 2008. Effects of light and nutrient availability on leaf mechanical properties of plantago major: A conceptual approach. Annals of Botany 101: 727-736.

Sass, J.E. 1958. Botanical Microtechnique. Edisi ke-3. Ames: Iowa State University Press.

Slivinske, S. 2008. Rafflesia breeding conservation. Hort 404-Plant Breeding.

Sofiyanti, N., Kamarudin, M.S., Khairil, M., Nor Zuhailah, M., Mohd Ros, A.H. & Burslem, D.F.R.P. 2016. Rafflesia parvimaculata (Rafflesiaceae), a new species of Rafflesia from Peninsular Malaysia. Phytotaxa 253(3): 207-213.

Tan, A.L. 2006. Kajian Rafflesia Kuala Melantai Pahang. Tesis Sarjana Muda, Universiti Kebangsaan Malaysia (Tidak diterbitkan).

Tan, S.Y., Koh, C.Y., Siow, H.J.M., Li, T., Wong, H.F., Heyzer, A. & Tan, H.T.W. 2013. 100 Common Vascular Plants of the Nee Soon Swamp Forest, Singapore. Singapore: Raffles Museum of Biodiversity Research.

Veldkamp, J.F. 2007. The correct name for the Tetrastigma (Vitaceae) host of Rafflesia (Rafflesiaceae) in Malesia and a (not so) new species. Reinwardtia 12(4): 261-265.

Wan Nuur Fatiha, W.Z., Aida Shafreena, A.P., Geri, C., Ramlah, Z. & Latiff, A. 2016. Tetrastigma diepenhorstii (Miq.) Latiff (Vitaceae), a new host of Rafflesia Tuan-mudae Becc. (Rafflesiaceae) in Borneo. Journal of Botany 2016: 1-6. 

Wen, J., Lu, L. & Boggan, J.K. 2013. Diversity and evolution of Vitaceae in the Philippines. Philippine Journal of Science Special Issue 142: 223-244.

Xi, Z., Wang, Y., Bradley, R.K., Sugumaran, M., Marx, C.J., Rest, J.S. & Davis, C.C. 2013. Massive mitochondrial gene transfer in a parasitic flowering plant clade. PLoS Genetics 9(2): 1-10.

*Pengarang untuk surat-menyurat; email: afiqaizat@ukm.edu.my