Sains Malaysiana 51(11)(2022): 3663-3676
http://doi.org/10.17576/jsm-2022-5111-12
Functional and Bioactive
Properties of Bupleurum chinense DC.
and Clematis chinensis Osbeck Mediated Biogenic Synthesized Silver
Nanoparticles
(Sifat
Fungsian dan Bioaktif Bupleurum chinense DC. dan Clematis chinensis Osbeck
antara Nanozarah Perak Biogen Disintesis)
TER BOON SIANG1,
SINOUVASSANE DJEARAMANE2, FANNE YEOH FERN NII1 & ANTO
CORDELIA TANISLAUS ANTONY DHANAPAL1,*
1Department of Chemical Science, Faculty
of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Perak Darul Ridzuan, Malaysia
2Centre for Biomedical and Nutrition
Research Department of Allied Health Sciences, Faculty of Science, Universiti Tunku Abdul Rahman,
31900 Kampar, Perak Darul Ridzuan,
Malaysia
Diserahkan: 22 November 2021/Diterima:
7 Julai 2022
Abstract
The synthesis of novel metallic nanoparticles (NPs)
using plant materials has emerged as a cost-effective
alternative to synthetic NPs and has found wide application in biomedical,
cosmetic, bioremediation, and health care technology. This study aimed to
synthesize and characterize the morphologies, functional groups, and
crystalline structures of silver nanoparticles (AgNPs)
deduced using B. chinense (Bc) and C. chinensis (Cc).
The biogenic AgNPs were appraised for their
antioxidant content, radical scavenging activities, antimicrobial,
anti-inflammatory, and anti-diabetic activities to understand their functional
and health promoting properties. The AgNPs deduced
from both plants were spherical in shape with the size of 20-30 nm and
possessed face centered cubic phase arrangement. The total phenolic content
(TPC) was high in Cc-AgNPs (166.2100 mgGAE/g) and exhibited strong
hydroxyl (OH-) and ABTS+ radical scavenging activities. On the contrary, Bc-AgNPs with higher TFC (286.1438 mgQE/g) had greater nitric-oxide (NO), DPPH, Fe2+scavenging and anti-albumin
denaturation activities. Minimum anti-α-amylase
activity disparity was observed between Cc-AgNPs(EC50 = 0.2903 mg/mL) and Bc-AgNPs(EC50 = 0.2975 mg/mL) due to various
inhibition mode contributed by bioactive compounds. Besides, Bc-AgNPs showed
strongest inhibition towards Gram negative bacteria while Cc-AgNPs was more effective as an antifungal agent. In conclusion, biogenic AgNPs exhibited strong
functional and bioactive properties which may be attributed to the presence of
bioactive compounds on the surface of AgNPs and
further in-depth analysis are required to be used for various biomedical
applications.
Keywords:
Antioxidant activity; anti-bacterial; biogenic synthesis; Bupleurum chinense DC.; Clematis chinensis Osbeck; silver nanoparticles
Abstrak
Sintesis nanozarah logam (NP) novel
menggunakan bahan tumbuhan telah muncul sebagai alternatif kos efektif kepada
NP sintetik dan telah menemui aplikasi meluas dalam teknologi bioperubatan,
kosmetik, biopemulihan dan penjagaan kesihatan. Kajian ini bertujuan untuk
mensintesis dan mencirikan morfologi, kumpulan berfungsi, dan struktur kristal
nanozarah perak (AgNPs) yang disimpulkan menggunakan B. chinense (Bc) dan C.
chinensis (Cc). AgNP biogenik dinilai untuk kandungan antioksidannya,
aktiviti penghapusan radikal, aktiviti antimikrob, anti-radang dan
anti-diabetes untuk memahami sifat fungsian dan kesihatannya. AgNP yang
disimpulkan daripada kedua-dua tumbuhan adalah berbentuk sfera dengan saiz
20-30 nm dan mempunyai susunan fasa kubik berpusat muka. Jumlah kandungan
fenolik (TPC) adalah tinggi dalam Cc-AgNPs (166.2100 mgGAE/g) dan mempamerkan
aktiviti penghapusan radikal hidroksil (OH-) dan ABTS+ yang kuat. Sebaliknya,
Bc-AgNPs dengan TFC yang lebih tinggi (286.1438 mgQE/g) mempunyai aktiviti
denaturasi nitrik-oksida (NO), DPPH, Fe2+ dan anti-albumin yang lebih besar.
Jurang aktiviti anti-α-amilase minimum diperhatikan antara Cc-AgNPs (EC50
= 0.2903 mg/mL) dan Bc-AgNPs (EC50 = 0.2975 mg/mL) disebabkan pelbagai mod
perencatan yang disumbangkan oleh sebatian bioaktif. Selain itu, Bc-AgNPs
menunjukkan perencatan paling kuat terhadap bakteria Gram negatif manakala
Cc-AgNPs lebih berkesan sebagai agen antikulat. Kesimpulannya, AgNP biogenik
mempamerkan sifat fungsian dan bioaktif yang kuat yang mungkin dikaitkan dengan
kehadiran sebatian bioaktif pada permukaan AgNPs dan analisis lebih mendalam
diperlukan untuk digunakan untuk pelbagai aplikasi bioperubatan.
Kata kunci: Aktiviti antioksida; anti-bakteria; Bupleurum chinense DC.; Clematis chinensis Osbeck; nanozarah perak; sintesis biogen
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*Pengarang untuk surat-menyurat; email: antoc@utar.edu.my