Sains Malaysiana 52(4)(2023):
1243-1258
http://doi.org/10.17576/jsm-2023-5204-16
Keberkesanan Tokoferol sebagai Antioksidan dalam
Ayam Salai
(Effectiveness
of Tocopherol as Antioxidant in Chicken Jerky)
CHIE CHING BOON1, LEE SIN CHANG2,3,
ABDUL SALAM BABJI1,3, CHRIS MUN FEI YAP4, JIA MIN TAM4 NOORUL SYUHADA MOHD RAZALI1,3 & SENG JOE LIM1,3,*
1Department
of Food Sciences, Faculty of Science and Technology, Universiti Kebangsaan
Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
2Department
of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University
Kuala Lumpur, No.1, Jalan Menara Gading, UCSI Heights 56000 Cheras, Kuala
Lumpur, Federal Territory, Malaysia
3Innovation
Centre for Confectionery Technology (MANIS), Faculty of Science and Technology,
Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
4Wing
Heong Food Industries Sdn Bhd, No. 2, Jalan 7/7 Taman Industri Selesa Jaya,
43300 Seri Kembangan, Selangor Darul Ehsan, Malaysia
Diserahkan: 5 Disember 2022/Diterima: 17 Mac 2023
Abstrak
Kajian ini dijalankan untuk mengenal pasti keberkesanan
tokoferol sebagai antioksidan dalam ayam salai. Perapan ayam telah disediakan
dengan tiga formulasi mengandungi tokoferol yang berbeza kepekatan iaitu 100
mg/kg, 200 mg/kg dan 300 mg/kg. Ujian jangka hayat dipercepat (ASLT) telah
dijalankan berdasarkan nilai Q10 yang dikenal pasti pada 1.30 dengan
penyimpanan sampel pada suhu 57 °C selama empat minggu adalah bersamaan dengan
hayat simpanan selama 61.5 hari pada suhu bilik. Hasil kajian menunjukkan
bahawa nilai peroksida (pengoksidaan primer) bagi semua sampel tidak berbeza
secara signifikan (p>0.05) manakala nilai TBARS (pengoksidaan sekunder) bagi
sampel ditambah tokoferol adalah lebih rendah secara signifikan (p<0.05)
berbanding sampel tanpa tokoferol. Ayam salai didapati mempunyai warna yang
lebih gelap dan pengurangan keamatan pada kemerahan dan kekuningan sepanjang
penyimpanan. Aktiviti air sampel ayam salai yang ditambah tokoferol adalah
tidak berbeza secara signifikan (p>0.05) berbanding dengan sampel tanpa
antioksidan. Semua sampel ayam salai menjadi lebih berasid sepanjang
penyimpanan dan menunjukkan perbezaan secara signifikan (p<0.05) manakala
jumlah kiraan plat bagi semua sampel adalah kurang daripada 106 cfu/g. Keputusan ujian sensori menunjukkan bahawa penerimaan keseluruhan sampel
ayam salai yang ditambah tokoferol, BHT dan sampel tanpa antioksidan tidak
menunjukkan perbezaan yang signifikan (p>0.05). Secara kesimpulannya, kajian
ini menunjukkan bahawa penambahan tokoferol sebagai antioksidan telah
mengurangkan tekanan oksidatif ayam salai dan formulasi perapan 300 mg/kg
tokoferol mempunyai potensi untuk dibangunkan sebagai produk ayam salai dengan
kestabilan oksidatif tinggi.
Kata kunci: Antioksidan; ayam salai; pengoksidaan lipid;
tokoferol
Abstract
This study was performed to determine the
effectiveness of tocopherol as antioxidant in chicken jerky. The marinade for
jerky was prepared in three formulations with different tocopherol contents at
100 mg/kg, 200 mg/kg and 300 mg/kg, as well as with synthetic antioxidant (BHT)
and without antioxidants. Accelerated shelf-life testing (ASLT) was performed
based on a pre-determined Q10 value of 1.30, where storage of the
jerky at 57 °C for 4 weeks are equivalent to 61.5 days storage at room temperature. The result obtained has shown that there was no
significant difference (p>0.05) in peroxide value (primary oxidation), while
TBARS value (secondary oxidation) of chicken jerky with tocopherol was
significantly (p<0.05) lower than sample without tocopherol. All chicken
jerky samples were found to have darker colour and loss intensity of redness
and yellowness throughout the storage. Chicken jerky with tocopherol showed
that there was no significant difference (p>0.05) in water activity compared
to sample without antioxidant. All the samples became acidic throughout the
storage and showed significant difference (p<0.05), while the total plate
count for all samples were less than 106 cfu/g.
There was no significant different (p>0.05) in overall acceptance of chicken
jerky with tocopherol, BHT and sample without antioxidant, during the sensory
evaluation. As a conclusion, this study showed that the addition of tocopherol
as antioxidant had reduced oxidative stress of chicken jerky and formulation
300 mg/kg tocopherol can be developed to produce chicken jerky product with
high oxidative stability.
Keywords: Antioxidant; chicken jerky; lipid oxidation; tocopherol
RUJUKAN
Atinafu
, D.G. & Bedemo, B. 2011. Estimation of total free fatty acid and
cholesterol content in some commercial edible oils in Ethiopia, Bahir DAR. Journal of Cereals Oilseeds 2(5): 71-76.
Azzi, A. 2007. Molecular mechanism of α-tocopherol
action. Free Radical Biology Medicine 43(1): 16-21.
Barriuso, B., Astiasarán, I. & Ansorena, D. 2013. A review
of analytical methods measuring lipid oxidation status in foods: A challenging
task. European Food Research Technology 236(1): 1-15.
Campo, M., Nute, G., Hughes, S., Enser, M., Wood, J. &
Richardson, R. 2006. Flavour perception of oxidation in beef. Meat Science 72(2): 303-311.
Chen, J., Hu, Y., Wen, R., Liu, Q., Chen, Q. & Kong, B.
2019. Effect of NaCl substitutes on the physical, microbial and sensory
characteristics of Harbin dry sausage. Meat
Science 156: 205-213.
Cheng, J.H., Sun, D.W., Pu, H. & Zhu, Z. 2015. Development
of hyperspectral imaging coupled with chemometric analysis to monitor K value
for evaluation of chemical spoilage in fish fillets. Food Chemistry 185: 245-253.
Choe, E. & Oh, S. 2013. Effects of water activity on the
lipid oxidation and antioxidants of dried laver (Porphyra) during storage in
the dark. Journal of Food Science 78(8): C1144-C1151.
Cui, L. & Decker, E.A. 2016. Phospholipids in foods: Prooxidants
or antioxidants? Journal of the Science
of Food Agriculture 96(1): 18-31.
Djenane, D., Sanchez-Escalante, A., Beltrán, J.A. &
Roncales, P. 2002. Ability of α-tocopherol, taurine and rosemary, in
combination with vitamin C, to increase the oxidative stability of beef steaks
packaged in modified atmosphere. Food
Chemistry 76(4): 407-415.
Domínguez, R., Pateiro, M., Gagaoua, M., Barba, F.J., Zhang,
W. & Lorenzo, J.M. 2019. A comprehensive review on lipid oxidation in meat
and meat products. Antioxidants 8(10): 429.
US Food & Drug Administration (USFDA). 2014. Water
activity (aw) in foods. Inspection Technical Guides.
https://www.fda.gov/inspections-compliance-enforcement-and-criminal-investigations/inspection-technical-guides/water-activity-aw-foods
Fernandez-Lopez, J., Zhi, N., Aleson-Carbonell, L.,
Pérez-Alvarez, J.A. & Kuri, V. 2005. Antioxidant and antibacterial
activities of natural extracts: Application in beef meatballs. Meat Science 69(3): 371-380.
Ferreira, P.S., Spolidorio, L.C., Manthey, J.A. & Cesar, T.B. 2016. Citrus flavanones prevent systemic inflammation and ameliorate oxidative stress in C57BL/6J mice fed high-fat diet. Food and Function 7: 2675-2681.
Fiego, D.L., Santoro, P., Macchioni, P., Mazzoni, D.,
Piattoni, F., Tassone, F. & De Leonibus, E. 2004. The effect of dietary
supplementation of vitamins C and E on the α-tocopherol content of
muscles, liver and kidney, on the stability of lipids, and on certain meat quality
parameters of the longissimus dorsi of rabbits. Meat Science 67(2): 319-327.
Gan, J.Y., Chang, L.S., Mat Nasir, N.A., Babji, A.S. & Lim, S.J. 2020. Evaluation of physicochemical properties, amino acid profile and bioactivities of edible bird's nest hydrolysate as affected by drying methods. LWT-Food Science and Technology 131: 109777.
Gibbs, G. 2015. Accelerated shelf life of a health bar
contained in different bio-based packaging materials. Master Thesis. Clemson
University (Unpublished).
Gray, J.I. & Monahan, F.J. 1992. Measurement of lipid
oxidation in meat and meat products. Trends
in Food Science Technology 3: 315-319.
Haniff, M., Yahaya, S.A., Aziz, N.S., Wan Aida, W.M., Sofian Seng, N.S., Abdul Rahman, H.A., Mohd Razali, N.S. & Lim, S.J. 2020. Development of carotenoid-rich mayonnaise using carotino oil. Journal of Food Processing and Preservation 44: e14688.
Heitschmidt, J.D. 2015. Quality attributes of ready-to-eat
bison meat snacks during 40 °C accelerated storage. Master Thesis. Kansas State
University (Unpublished).
Higgins, F., Kerry, J., Buckley, D. & Morrissey, P. 1998.
Effect of dietary α-tocopheryl acetate supplementation on
α-tocopherol distribution in raw turkey muscles and its effect on the
storage stability of cooked turkey meat. Meat
Science 50(3): 373-383.
Hong, H., Luo, Y., Zhou, Z. & Shen, H. 2012. Effects of
low concentration of salt and sucrose on the quality of bighead carp (Aristichthys
nobilis) fillets stored at 4 °C. Food
Chemistry 133(1): 102-107.
Inchingolo, R., Cardenia, V. & Rodriguez‐Estrada,
M.T. 2013. The effects of microwave heating on edible oils and
lipid‐containing food. Lipid
Technology 25(3): 59-61.
İpek, G., Kavuşan, H.S., Serdaroğlu, M. &
Öztürk-Kerimoğlu, B. 2019. Incorporation of gelled emulsions with
α-tocopherol as fat replacer in heat-treated fermented sausages. 65th
International Congress of Meat Science and Technology Conference, Berlin,
Germany.
Isa, N.I.M., Ibrahim, N.N.A., Sabran, S.N.J., Sofian-Seng, N.S., Lim, S.J., Rahman, H.A., Wan Mustapha, W.A. & Razali, N.S.M. 2022. Kesan kepekatan fukoidan dan tempoh penapaian terhadap pencirian fizikokimia kombucha diperkuat dengan fukoidan. Sains Malaysiana 51(10): 3295-3306.
Jaimez-Ordaz, J., Pérez-Flores, J.G., Castaneda-Ovando, A., González-Olivares, L.G., Anorve-Morga, J. & Contreras-Lopez, E. 2019. Kinetic parameters of lipid oxidation in third generation (3G) snacks and its influence on shelf-life. Food Science Technology 39: 136-140.
Jin, G., He, L., Zhang, J., Yu, X., Wang, J. & Huang, F.
2012. Effects of temperature and NaCl percentage on lipid oxidation in pork
muscle and exploration of the controlling method using response surface
methodology (RSM). Food Chemistry 131(3): 817-825.
Kim, S.M., Kim, T.K., Kim, H.W., Jung, S., Yong, H.I. &
Choi, Y.S. 2021. Quality characteristics of semi-dried restructured jerky
processed using super-heated steam. Foods 10(4): 762.
Kong, J., Perkins, L.B., Dougherty, M.P. & Camire, M.E.
2011. Control of lipid oxidation in extruded salmon jerky snacks. Journal of Food Science 76(1): C8-C13.
Kumar, Y., Yadav, D.N., Ahmad, T. & Narsaiah, K. 2015.
Recent trends in the use of natural antioxidants for meat and meat products. Comprehensive Reviews in Food Science Food
Safety 14(6): 796-812.
Labuza, T.P. 1984. Application of chemical kinetics to
deterioration of foods. Journal of Chemical Education 61(4): 348-358.
Labuza, T.P. 1982. Shelf-life
Dating of Foods. Westport: Food & Nutrition Press, Inc.
Landes, N. 2005. Vitamin E: Elucidation of the mechanism of
side chain degradation and gene regulatory functions. PhD Thesis. Universität
Potsdam (Unpublished).
Leanwala, A.P. 2022. Application of extremophiles in food
industries. In Physiology, Genomics, and Biotechnological Applications of
Extremophiles, edited by Gunjal, A.B., Thombre, R. & Parray, J.A. IGI
Global. pp. 251-259.
Lee, J.H., Alford, L., Kannan, G. & Kouakou, B. 2017.
Curing properties of sodium nitrite in restructured goat meat (chevon) jerky. International
Journal of Food Properties 20(3): 526-537.
Lee, S.Y., Lee, D.Y., Kim, O.Y., Kang, H.J., Kim, H.S. &
Hur, S.J. 2020. Overview of studies on the use of natural antioxidative
materials in meat products. Food Science
of Animal Resources 40(6): 863-880.
Lekjing, S. 2016. A chitosan-based coating with or without
clove oil extends the shelf life of cooked pork sausages in refrigerated
storage. Meat Science 111: 192-197.
Leskovec, J., Levart, A., Svete, A.N., Perić, L.,
Stojčić, M.Đ., Žikić, D., Salobir, J. & Rezar, V. 2018.
Effects of supplementation with α-tocopherol, ascorbic acid, selenium, or
their combination in linseed oil-enriched diets on the oxidative status in
broilers. Poultry Science 97(5):
1641-1650.
Lim, H., Kim, G., Jung, E., Seo, H., Joo, S., Jin, S. &
Yang, H. 2014. Effect of curing time on the physicochemical and sensory
properties of beef jerky replaced salt with soy sauce, red pepper paste and
soybean paste. Asian-Australasian Journal
of Animal Sciences 27(8): 1174-1180.
Loo, Y.Y., Lim, H.Y., Sofian-Seng, N.S., Wan Mustapha, W.A. & Mohd Razali, N.S. 2022. Physicochemical characteristics and microbiological quality of silkworm (Bombyx mori) larval and pupae powder: Comparative study. Sains Malaysiana 51(2): 547-558.
Luckose, F., Pandey, M.C. & Harilal, P.T. 2017. Effect of
sodium chloride reduction on drying kinetics of restructured chicken jerky. Food Bioscience 19: 156-162.
Mahesar, S., Sherazi, S., Khaskheli, A.R. & Kandhro, A.A.
2014. Analytical approaches for the assessment of free fatty acids in oils and
fats. Analytical Methods 6(14):
4956-4963.
Manessis, G., Kalogianni, A.I., Lazou, T., Moschovas, M.,
Bossis, I. & Gelasakis, A.I. 2020. Plant-derived natural antioxidants in
meat and meat products. Antioxidants 9(12): 1215.
Martuscelli, M., Lupieri, L., Sacchetti, G., Mastrocola, D.
& Pittia, P. 2017. Prediction of the salt content from water activity
analysis in dry-cured ham. Journal of
Food Engineering 200: 29-39.
Masuda, T., Inaba, Y. & Takeda, Y. 2001. Antioxidant mechanism
of carnosic acid: Structural identification of two oxidation products. Journal of Agricultural Food Chemistry 49(11): 5560-5565.
Mazur-Kuśnirek, M., Antoszkiewicz, Z., Lipiński, K.,
Kaliniewicz, J. & Kotlarczyk, S. 2019. The effect of polyphenols and
vitamin E on the antioxidant status and meat quality of broiler chickens fed
low-quality oil. Archives Animal Breeding 62(1): 287-296.
Moemeni, F. & Yazdanpanah, S. 2020. Oxidative stability,
color, and physicochemical and sensorial properties of raw stacked and ground
meat treated with Shahpouri orange juice. Journal
of Food Quality 2020: 8886527.
Nacak, B., Kavusan, H., Sarı, B., Can, H. &
Serdaroglu, M. 2019. Collaborative effect of fat reduction and
α-tocopherol incorporation on oxidative stability in beef sausages. IOP Conference Series: Earth and
Environmental Science. p. 012084.
Ndia, Y.M.D., Ina, Y.T. & Kaka, A. 2021. Concentration of
lontar sugar (Borassus flabellifer Linn) and its effect on
physicochemical and organoleptic properties of village chicken jerky. Jurnal
Pangan dan Agroindustri 9(4): 251-259.
Ng, S.R., Noor, H.S.M., Ramachandran, R., Tan, H.Y., Ch’ng, S.E., Chang, L.S., Babji, A.S. & Lim, S.J. 2020. Recovery of glycopeptides by enzymatic hydrolysis of edible bird’s nest: The physicochemical characteristics and protein profile. Journal of Food Measurement and Characterization 14: 2635-2645.
Nielsen, S. 2005. Handbook of Food Analysis, Physical
Characterization and Nutrient Analysis. Wiley Online Library.
Nyström, L., Achrenius, T., Lampi, A.M., Moreau, R.A. &
Piironen, V. 2007. A comparison of the antioxidant properties of steryl
ferulates with tocopherol at high temperatures. Food Chemistry 101(3):
947-954.
Özilgen, S. & Özilgen, M. 1990. Kinetic model of lipid oxidation in foods. Journal of Food Science 55(2): 498-498.
Rahayu, W., Martono, S. & Rohman, A. 2018. The potential
use of infrared spectroscopy and multivariate analysis for differentiation of
beef meatball from dog meat for Halal authentication analysis. Journal of Advanced Veterinary Animal
Research 5(3): 307-314.
Ramlan, N.A.F.M., Mohammad, S.M., Karim, R., Muhammad, S.K.S., Ismail, M. & Zawawi, N. 2021. Nutritional composition, techno-functional properties and sensory analysis of pan bread fortified with kenaf seeds dietary fibre. Sains Malaysiana 50(11): 3285-3296.
Rezaeifar, M., Mehdizadeh, T., Mojaddar Langroodi, A. &
Rezaei, F. 2020. Effect of chitosan edible coating enriched with lemon verbena
extract and essential oil on the shelf life of vacuum rainbow trout (Oncorhynchus
mykiss). Journal of Food Safety 40(3): e12781.
Ruan, D., Wang, H. & Cheng, F. 2018. The Maillard
Reaction in Food Chemistry: Current Technology and Applications. Springer
International Publishing.
Sattler, S.E., Gilliland, L.U., Magallanes-Lundback, M.,
Pollard, M. & Dellapenna, D. 2004. Vitamin E is essential for seed
longevity and for preventing lipid peroxidation during germination. The Plant Cell 16(6): 1419-1432.
Selani, M., Contreras-Castillo, C., Shirahigue, L., Gallo, C.,
Plata-Oviedo, M. & Montes-Villanueva, N. 2011. Wine industry residues extracts
as natural antioxidants in raw and cooked chicken meat during frozen storage. Meat Science 88(3): 397-403.
Silva, F.A., Estévez, M., Ferreira, V.C., Silva, S.A., Lemos,
L.T., Ida, E.I., Shimokomaki, M. & Madruga, M.S. 2018. Protein and lipid
oxidations in jerky chicken and consequences on sensory quality. LWT 97: 341-348.
Sorapukdee, S., Uesakulrungrueng, C. & Pilasombut, K. 2016. Effects of humectant and roasting on physicochemical and sensory properties of jerky made from spent hen meat. Korean Journal for Food Science of Animal Resources 36(3): 326.
Silva, F.A., Estévez, M., Ferreira, V.C., Silva, S.A., Lemos,
L.T., Ida, E.I., Shimokomaki, M. & Madruga, M.S. 2017. Characterization of
preserved meat from spent hen and broiler by salting and forced‐air
drying. Journal of Food Processing
Preservation 41(4): e13048.
Suwansri, S., Ratanatriwong, P. & Thanasukarn, P. 2009. Development of crystallized palm-syrup sugar as a natural sweetener. Asian Journal of Food and Agro-Industry SI: S130-S136.
Syamaladevi, R.M., Tadapaneni, R.K., Xu, J., Villa-Rojas, R.,
Tang, J., Carter, B., Sablani, S. & Marks, B. 2016. Water activity change
at elevated temperatures and thermal resistance of Salmonella in all
purpose wheat flour and peanut butter. Food
Research International 81: 163-170.
Waltzeko, P. & Labuza, T. 1976. Accelerated shelf-life testing of an intermediate moisture food in air and in an oxygen-free atmosphere. Journal of Food Science 41(6): 1338-1344.
Wang, L.L. & Xiong, Y.L. 2005. Inhibition of lipid
oxidation in cooked beef patties by hydrolyzed potato protein is related to its
reducing and radical scavenging ability. Journal
of Agricultural Food Chemistry 53(23): 9186-9192.
Waskitho, D., Lukitaningsih, E. & Rohman, A. 2016.
Analysis of lard in lipstick formulation using FTIR spectroscopy and
multivariate calibration: A comparison of three extraction methods. Journal of Oleo Science 65(10): 815-824.
Wen, R., Lv, Y., Li, X.A., Chen, Q. & Kong, B. 2021. High-throughput
sequencing approach to reveal the bacterial diversity of traditional yak jerky
from the Tibetan regions. Meat Science 172: 108348.
Xiong, Q., Zhang, M., Wang, T., Wang, D., Sun, C., Bian, H.
& Xu, W. 2020. Lipid oxidation induced by heating in chicken meat and the
relationship with oxidants and antioxidant enzymes activities. Poultry
Science 99(3): 1761-1767.
Yang, C. & Chen, T.C. 1993. Effects of refrigerated
storage, pH adjustment, and marinade on color of raw and microwave cooked
chicken meat. Poultry Science 72(2): 355-362.
Yilmaz, B. & Ozturk, M. 2004. Comparison of two derivative spectrophotometric methods for the determination of a-tocopherol in pharmaceutical preparations. Il Farmaco 59(9): 723-727.
Zdanowska-Sąsiadek, Ż., Damaziak, J.M., Marchewka,
J., Horbańczuk, O., Jóźwik, A., Wójcik, W. & Horbańczuk, J.
2022. Lipid-and protein oxidation during storage and in vitro gastrointestinal digestion of ostrich, beef and chicken jerky snacks. Animal
Science Papers and Reports 40(3): 305-316.
Zelenka, J., Jarošová, A. & Schneiderová, D. 2008.
Influence of n-3 and n-6 polyunsaturated fatty acids on sensory characteristics
of chicken meat. Czech Journal of Animal
Science 53(7): 299-305.
Zhang, H., Wu, J. & Guo, X. 2016. Effects of antimicrobial
and antioxidant activities of spice extracts on raw chicken meat quality. Food
Science and Human Wellness 5(1): 39-48.
*Pengarang untuk surat-menyurat; email: joe@ukm.edu.my
|