Sains Malaysiana 52(5)(2023):
1397-1405
http://doi.org/10.17576/jsm-2023-5205-06
Toxicity
of Clove Oil Nanoparticles against Diamondback Moth Plutella xylostella (L.)
(Ketoksikan Nanozarah Minyak Cengkih terhadap Rama-rama Berlian Plutella xylostella (L.))
SILVI IKAWATI* & FERY ABDUL CHOLIQ
Department of
Plant Pest and Disease, Faculty of Agriculture, University of Brawijaya, Jl.
Veteran, Malang 65145, East Java, Indonesia
Diserahkan:
11 April 2022/Diterima: 19 April 2023
Abstract
Plutella
xylostella, diamondback moth (DBM), has been one of the most
challenging insects to control in the world to date. Environmentally
friendly methods of control, such as the use of botanical insecticides, are
available. A formulation that can sustain the main
compound's level is required, which can be accomplished through soluble powder nanoformulation. The
goal of this research was to test and evaluate the ability of clove oil
nanoparticles produced from polyethylene glycol 6000 (PEG 6000) to control DBM
utilizing a solid dispersion technique. Bioassay by leaf dip method in laboratory was used to
test the lethal effect of clove oil nanoparticles (CO-NPs) on DBM. Clove
oil nanoformulation was successful because it produced nanoparticles (179.98 nm in diameter) while maintaining high levels of the active component
eugenol. Clove oil nanoparticles may increase
clove oil's toxicity to DBM, seen from the LC50 value after 24 h of
treatment. The LC50 values for clove oil nanoparticles
after 24 and 48 h of treatment were 10.308 and 9.451%, respectively.
Keywords: Botanical
pesticides; clove oil; nanoformulation; Plutella
xylostella; solid dispersion
Abstrak
Plutella
xylostella, rama-rama
berlian (DBM), telah menjadi salah satu serangga yang paling mencabar untuk
dikawal di dunia setakat ini. Kaedah kawalan mesra alam, seperti penggunaan
racun serangga botani, tersedia. Formulasi yang boleh mengekalkan tahap
sebatian utama diperlukan, yang boleh dicapai melalui nanoformulasi pepejal.
Matlamat penyelidikan ini adalah untuk menguji dan menilai keupayaan nanozarah
minyak cengkih yang dihasilkan daripada polietilena glikol 6000 (PEG 6000)
mengawal DBM menggunakan teknik penyebaran pepejal. Bioassay melalui kaedah
celup daun di makmal digunakan untuk menguji kesan maut nanozarah minyak cengkih
(CO-NPs) pada DBM. Nanoformulasi minyak cengkih berjaya kerana ia menghasilkan
nanozarah (diameter 179.98 nm) sambil mengekalkan tahap tinggi komponen aktif
eugenol. Nanozarah minyak cengkih boleh meningkatkan ketoksikan minyak cengkih
kepada DBM, dilihat daripada nilai LC50 selepas 24 jam rawatan.
Nilai LC50 untuk nanozarah minyak cengkih selepas 24 dan 48 jam
rawatan masing-masing adalah 10.308 dan 9.451%.
Kata kunci: Formulasi nano;
minyak cengkih; penyebaran pepejal; Plutella
xylostella; racun serangga botani
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*Pengarang
untuk surat-menyurat; email: silviikawati@ub.ac.id
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