Sains Malaysiana 52(5)(2023): 1407-1417

http://doi.org/10.17576/jsm-2023-5205-07

Filem Mikrosfera Akrilik Terpegun Alizarin Merah S untuk Pengesanan Pendarfluor Optik Asid Borik 

(Alizarin Red S Immobilized Acrylic Microspheres Film for Optical Fluorescence Sensing of Boric Acid)

RAJA ZAIDATUL AKHMAR RAJA JAMALUDDIN^1,*, MUSA AHMAD², LEE YOOK HENG¹ & LING LING TAN³

¹Jabatan Sains Kimia, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

²Fakulti Sains dan Teknologi, Universiti Sains Islam Malaysia, Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan Darul Khusus, Malaysia

³Pusat Kajian Bencana Asia Tenggara (SEADPRI), Institut Alam Sekitar dan Pembangunan (LESTARI), Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

Diserahkan: 12 Februari 2023/Diterima: 2 Mei 2023

Abstrak

Mikrosensor pendarfluor optik untuk kuantifikasi asid borik dalam sampel makanan telah dibangunkan berdasarkan filem mikrosfera poli(n-butil akrilat) [poli(nBA)]. Pencirian optik reagen alizarin merah S (ARS) terpegun pada filem mikrosfera poliakrilat dengan asid borik telah dilakukan melalui pendekatan transduksi pendarfluor. Reagen ARS telah dijerap secara fizikal pada filem mikrosfera akrilik hidrofobik untuk membentuk mikropolimer kelat dengan sokongan matriks sol-gel untuk mengelakkan larut lesap molekul ARS yang bersifat larut air. Pengkelat ARS yang terpegun pada filem mikrosfera poli(nBA) memberikan tindak balas pengesanan optik yang cepat dalam masa 1 minit. Julat rangsangan linear dinamik yang berguna bagi mikrosensor pendarfluor optik asid borik telah diperoleh antara 1.6 µM dan 32.0 µM asid borik dengan had pengesanan (LOD) pada 1 μM. Mikrosensor pendarfluor optik asid borik berasaskan mikropolimer akrilik menghasilkan prestasi kebolehulangan yang baik dengan nilai sisihan piawai relatif (RSD) yang dicapai dalam julat 3.3-3.6%. Mikrosensor optik yang dibangunkan telah digunakan untuk pengukuran pendarfluor asid borik dalam sampel mi kuning dan mi beras leper ('hor fun'/'kway teow') dan tidak menunjukkan perbezaan yang ketara berbanding dengan kaedah rujukan tradisional spektrometri pemancaran plasma-optik berganding secara induktif (ICP-OES).

Kata kunci: Alizarin Merah S; asid borik; mikrosfera akrilik; pendarfluor; sensor optik

Abstract

An optical microsensor for the quantification of boric acid in food samples has been developed based on poly(n-butyl acrylate) [poly(nBA)] microspheres film. Optical characterization of the immobilized alizarin red S (ARS) reagent on the polyacrylate microspheres film with boric acid has been performed via a fluorescence transduction approach. The ARS reagent was physically adsorbed on the hydrophobic acrylic microspheres film to form a chelating micropolymer with the support of a sol-gel matrix to prevent the leaching of the water-soluble ARS molecules. The immobilized ARS chelator on the poly(nBA) microspheres film afforded a quick optical sensing response within 1 min. A useful dynamic linear response range of the optical microsensor was established between 1.6 µM and 32.0 µM boric acid with a limit of detection (LOD) obtained at 1 μM. The acrylic micropolymer-based fluorescence boric acid sensor yielded promising reproducibility resullts with relative standard deviation (RSD) values attained in the range of 3.3-3.6%. The developed optical chemical microsensor has been applied for fluorescence quantitation of boric acid in yellow noodle and flat rice noodle (‘hor fun’/’kway teow’) samples, and exhibited no significant disagreement compared with traditional inductively coupled plasma-optical emission spectrometry (ICP-OES) reference method.

Keywords: Alizarin Red S; acrylic microspheres; boric acid; fluorescence; optical chemical sensor

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