Malaysian Journal of Analytical Sciences Vol 19 No 6 (2015): 1218 - 1222

 

 

 

Biological Properties of Cucumber (Cucumis sativus L.) Extracts

 

(Sifat-Sifat Biologi Ekstrak Timun (Cucumis sativus L.))

 

Fiona Ni Foong How¹*, Aqeelah Mohammad¹, Solachuddin Jauhari Arief Ichwan²

 

¹Department of Chemistry, Kulliyyah of Science

²Department of Basic Medical Sciences, Kulliyyah of Dentistry

International Islamic University Malaysia,

Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia

 

*Corresponding author: howfiona@iium.edu.my

 

 

Received: 9 December 2014; Accepted: 16 October 2015

 

 

Abstract

Peel and pulp of Cucumis sativus L. fruit were extracted from aqueous (water) and phosphate buffered solution at incubated temperature of 37°C similar to normal human physiological temperature to investigate the potential of these extracts as antibacterial and cytotoxic agents using antibacterial susceptibility assay against six pathogenic bacteria of gram positive and negative and cytotoxic assay against human non-small cell lung carcinoma cell line (H1299) and human breast adenocarcinoma cell line (MCF-7). The phytochemical contents of all extracts were determined to correlate with their biological properties.

 

Keywords: Cucumis sativus L., biological activities, anticancer, aqueous cucumber extract

 

Abstrak

Kulit dan pulpa buah Cucumis sativus L. telah diekstrak dengan menggunakan akueus (air) and larutan penimbal fosfat yang sedang dieram pada suhu 37°C sama dengan suhu normal fisiologi manusia untuk mengkaji potensi pelbagai ekstrak tersebut sebagai agen antibakteria and agen sitotoksik dengan menggunakan asai kelemahan antibakteria terhadap enam bakteria patogenik gram positif dan negatif dan asai sitotoksik terhadap sel karsinoma paru-paru bukan kecil manusia (H1299) dan sel adenokarsinoma payudara manusia (MCF-7). Kandungan fitokimia dalam semua ekstrak telah ditentukan supaya berhubung kait dengan sifat-sifat biologi.

 

Kata kunci: Cucumis sativus L., aktiviti biologi; anti-kanser; ekstrak akueus timun

 

References

1.       Chu, Y. F., Sun, J., Wu, X. and Liu, R. H. (2002) Antioxidant and antiproliferative activities of common vegetables. J Agric. Food Chem., 50: 6910-6916.

2.       Repka, V. and Fischerova, I. (1999) Induction and distribution of amylolytic activity in Cucumis sativus L. in response to virus infection. Acta Virol., 43: 227-235.

3.       Saidu, A. N., Oibiokpa, F. I. and Olukotun I. O. (2014) Phytochemical screening and hypoglycemic effect of methanolic fruit pulp extract of Cucumis sativus in alloxan induced diabetic rats. J. Med. Plants Res. 8(39): 1173-1178.

4.       Mallik, J. and Akhter, R. (2012) Phytochemical Screening and In-vitro Evaluation of Reducing Power, Cytotoxicity and Anti-Fungal Activities of Ethanol Extracts of Cucumis sativus. Int. J. Pharm. Biol. Sci. Arch., 3(3): 555-560.

5.       Quek, S. Y., Chok, N. K. and Swedlund, P. (2007) The physicochemical properties of spray-dried watermelon powders. Chemical Engineering Principles in Food Industry, Chemical Engineering and Processing: Process Intensification 46(5): 386–392.

6.       Harborne, J. B. (1998) Phytochemical Methods - A Guide to Modern Techniques of plant analysis. London: Chapman and Hall.

7.       Tiwari, P., Kumar, B., Kaur, M., Kaur, G. and Kaur, H. (2011) Phytochemical screening and Extraction: A Review. Internationale Pharmaceutica Sciencia. 1(1): 98-106.

8.       Bauer, W., Kirby, M. D. K., Sherris, J. C. and Turck, M. (1966) Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Path., 45: 493-496.

9.       Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J.  Immunol. Meth., 65(1-2): 55-63.

10.    Maatalah, M. B., Bouzidi, N. K., Bellahouel, S., Merah, B., Fortas, Z., Soulimani, R., Saidi, S. and Derdour A. (2012) Antimicrobial activity of the alkaloids and saponin extracts of Anabasis articulate. J. Biotechnol. Pharm. Res. 3 (3): 54-57.

11.    Ates, D. A. and Erdogrul, O. T. (2003) Antimicrobial Activities of Various Medicinal and Commercial Plant Extracts. Tr J. Biology. 27: 157-162.

12.    Ighodaro, O.M., Agunbiade, S. O. and Akintobi, O.A. (2010) Phytotoxic and Anti-Microbial Activities of Flavonoids in Ocimum gratissimum. Eur J. Appl. Sci. 2(1): 37-40.

13.    Podolak, A. Galanty and Sobolewska, D. (2010) Saponins as cytotoxic agents: a review. Phytochem. Rev. 9: 425–474.

14.    Heidari, H., Kamalinejad, M. & Eskandari, M. (2012). Hepatoprotective activity of Cucumis sativus against cumene hydroperoxide induced-oxidative stress. Res. Pharm. Sci. 7(5): S936-S939.