Malaysian Journal of Analytical Sciences Vol 20 No 3 (2016): 546 - 550

DOI: http://dx.doi.org/10.17576/mjas-2016-2003-12

 

 

 

PHYTOCHEMICAL STUDY ON THE STEM BARK OF

Mallotus leucodermis Hook F.

 

(Kajian Fitokimia ke atas Kulit Ranting Mallotus leucodermis Hook F.)

 

Aiza Syuhada Mohd Yusoff ¹, Norizan Ahmat ^{1, 2}*, Humera Naz²

 

¹Department of Chemistry, Faculty of Applied Sciences,

Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia

²Atta ur Rahman Institute for Natural Products Discovery,

Universiti Teknologi MARA, 42300 Bandar Puncak Alam, Selangor, Malaysia

 

*Corresponding author: noriz118@salam.uitm.edu.my

 

 

Received: 24 February 2015; Accepted: 27 October 2015

 

 

Abstract

The stem barks of Mallotus leucodermis Hook F. (Euphorbiaceae) was studied for its chemical constituents.  The air dried and pulverized of stem bark of M. leucodermis (1.3 kg) was extracted successively with acetone for three days at room temperature yielding 66.0 g of crude extract. The crude extract was fractionated using vacuum liquid chromatography (VLC) to afford six fractions. Fraction 6 was further washed and recrystallized to afford bergenin (1). Fraction 3 was subjected to multiple purification using radial chromatography (CHCl₃: acetone) with different ratio 9:1, 8:2, 6:4 and 5:5 to yield a flavonoid compound, epicatechin (2). These compounds were elucidated based on spectroscopic analysis (Ultra Violate, Infra-Red, Mass Spectrometry and Nuclear Magnetic Resonance) as well as comparison with literatures.

 

Keywords: Euphorbiaceae, Mallotus, bergenin, epicatechin

 

Abstrak

Kulit ranting Mallotus leucodermis Hook F. (Euphorbicaeae) telah dikaji untuk sebatian kimianya. Kulit ranting M. leucodermis (1.3 kg) yang telah dikeringkan dan dikisar, direndamkan dalam aseton selama tiga hari pada suhu bilik bagi memperolehi 66.0 g bahan ekstrak. Fraksinasi telah dilakukan ke atas ekstrak tersebut dgn menggunakan hampagas kromatografi cecair dan menghasilkan enam fraksi. Fraksi 6 telah dicuci beberapa kali dan dihablurkan semula untuk menghasilkan bergenin (1). Manakala fraksi 3 ditulenkan beberapa kali menggunakan radial kromatografi untuk mendapatkan sebatian flavonoid, epicatechin (2). Dua sebatian tulen ini dikenal pasti berdasarkan analisis spektroskopi (Ultralembayung, Inframerah, Spektrometri Jisim and Resonans Magnet Nukleus) dan juga perbandingan data.

 

Kata kunci: Euphorbiaceae, Mallotus, bergenin, epicatechin

 

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