Malaysian Journal of Analytical Sciences Vol 20 No 5 (2016): 1181 - 1190

DOI: http://dx.doi.org/10.17576/mjas-2016-2005-25

 

 

 

PHYTOCHEMICAL SCREENING, TOTAL FLAVONOID AND PHENOLIC CONTENT ASSAYS OF VARIOUS SOLVENT EXTRACTS OF TEPAL OF Musa paradisiaca

 

(Penyaringan Fitokimia, Jumlah Asai Kandungan Flavonoid dan Fenolik Pelbagai Ekstrak Pelarut Tepal Musa paradisiaca)

 

U.S. Mahadeva Rao¹*, Muhammad Abdurrazak¹, Khamsah Suryati Mohd²

 

¹Faculty of Medicine,

Universiti Sultan Zainal Abidin, Kota Campus, 20400 Kuala Terenganu, Terenganu, Malaysia

²Faculty of Bioresources and Food Industry,

Universiti Sultan Zainal Abidin, Tembila Campus, 22200 Besut, Terenganu, Malaysia

 

*Corresponding author: raousm@gmail.com

 

 

Received: 14 April 2015; Accepted: 3 August 2016

 

 

Abstract

The objective of this research is to conduct the preliminary phytochemical screening, total flavonoid and phenolic contents assays of various solvent extracts of tepal of Musa paradisiaca. Phytochemical screening was carried out according to the method of Trease and Evans, total flavonoid content was measured by the aluminium chloride colorimetric assay and total phenolic content was estimated spectrophotometrically by Folin-Ciocalteau method. - Preliminary phytochemical screening reveals the presence of phenolics, flavonoids, alkaloids, tannins, terpenoids in all the three different extracts (methanolic, ethanolic and aqueous). Tepal methanolic extract has the richest content of both phenolics and flavonoids i.e. (4.27 mg GAE/g and 0.25 mg QE/g) respectively, and aqueous extract was the least i.e. (1.32 mg GAE/g and 0.164 mg QE/g). All the extracts were not significantly different with one another (p >0.05). It can be hypothesised that the high contents of phenolic compounds of tepals of Musa paradisiaca indicated that these compounds contribute to the antioxidant activity and can be regarded as promising plant species for natural sources of radical scavenging activity with potential value for treatment of many life threatening diseases.

 

Keywords:  phytochemical, flavonoid, phenolics, Musa paradisiaca

 

Abstrak

Objektif kajian ini adalah untuk menjalankan pemeriksaan awal ke atas ketulenan fitokimia, jumlah kandungan fenolik flavonoid dan pelbagai ekstrak pelarut tepal Musa paradisiaca. Penyaringan fitokimia telah dilaksanakan mengikut kaedah Trease dan Evans, dimana jumlah kandungan flavonoid diukur oleh ujian kalorimetrik aluminium klorida dan jumlah kandungan fenolik dianggarkan secara spektrofotometer dengan kaedah Folin-Ciocalteau. Penyaringan awal fitokimia menunjukkan kehadiran fenolik, flavonoid, alkaloid, tannin dan terpenoid dalam ketiga – tiga ekstrak yang berbeza (metanol, etanol dan akueus). Ekstrak metanol Tepal mempunyai kandungan yang paling tinggi di antara kedua-dua fenolik dan flavonoid iaitu sebanyak 4.27 mg GAE/g dan 0.25 mg QE/g, manakala ekstrak akueus adalah mempunyai kandungan terendah iaitu sebanyak 1.32 mg GAE/g dan 0,164 mg QE/g. Semua ekstrak tidak berbeza secara ketara antara satu sama lain (p >0.05). Oleh itu, hipotesis yang boleh dibuat adalah kandungan yang tinggi sebatian fenolik daripada tepal Musa paradisiaca menyumbang kepada aktiviti antioksidan dan boleh dianggap sebagai spesies tumbuhan yang berpotensi untuk merawat pelbagai penyakit yang mengancam nyawa.

 

Kata kunci:  fitokimia, flavonoid, fenolik, Musa paradisiaca

 

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