Malaysian Journal of Analytical Sciences Vol 21 No 1 (2017): 27 - 36

DOI: http://dx.doi.org/10.17576/mjas-2017-2101-04

 

 

 

A SINGLE HPLC METHOD FOR SEPARATION OF OLIGOSTILBENES FROM DIFFERENT DIPTEROCARPACEAE EXTRACTS

 

(Kaedah KCPT untuk Pemisahan Oligostilbene dari Ekstrak Dipterocarpaceae yang Berbeza)

 

Erni Muis¹, Rohaity Ramli^1,2, Nurhuda Manshoor^1,2*

 

¹Faculty of Pharmacy

²Atta-ur-Rahman Institute for Natural Products Discovery

Universiti Teknologi MARA Selangor, Puncak Alam Campus, 43200 Puncak Alam, Selangor, Malaysia

 

*Corresponding author: nurhuda15@salam.uitm.edu.my

 

 

Received: 3 October 2016; Accepted: 24 November 2016

 

 

Abstract

One standard chromatographic conditions of HPLC was developed for different Dipterocarpaceae plant extracts. The purpose of this study is to achieve an optimized chromatographic method that is able to produce a reasonably good separation and resolution of oligostilbenes in plant crude extracts. For this purpose, five model samples of Dipterocarpaceae extracts, Shorea parvifolia, Shorea ovalis, Dipterocarpus crinitus, Dipterocarpus gracilis and Hopea spp. were used. The parameters, which include types of chromatographic solvents, types of mobile phase elution, solvent composition and the injection volumes, were modified. The results showed that the optimum chromatographic conditions were achieved when a gradient elution was adopted, with the solvent composition of H₂O: ACN from 95:5 to 25:75 in 20 minutes. The injection volume of 5 μL is best for a 1 mg/ml sample concentration. The temperature was maintained at 35 °C and the flow rate was at 1.0 ml/min. Some compromises were taken into account such as having less accurate method if compared with a single method developed for single extract or sample. The method showed positive results when tested on 10 extracts of the same family from different plant parts and localities.

 

Keywords:  dipterocarpaceae, Shorea spp., Dipterocarpus spp., chromatography, high performance liquid chromatography

 

Abstrak

Satu kaedah piawai untuk keadaan kromatografi KCPT telah dibina untuk ekstrak dari tumbuhan Dipterocarpaceae yang berlainan. Tujuan kajian ini ialah untuk mencapai satu kaedah kromatografi optimum yang dapat menghasilkan pemisahan komponen oligostilbene dari ekstrak tumbuhan dengan resolusi yang baik. Untuk tujuan ini, lima sampel ekstrak tumbuhan Dipterocarpaceae, Shorea parvifolia, Shorea ovalis, Dipterocarpus crinitus, Dipterocarpus gracilis dan Hopea spp. telah digunakan sebagai model. Parameter yang merangkumi jenis pelarut, jenis aliran fasa bergerak, komposisi pelarut dan isipadu suntikan sampel telah diubahsuai. Keputusan menunjukkan kaedah kromatografi yang sesuai berjaya dicapai apabila aliran bercerun digunakan dengan komposisi pelarut H₂O: ACN dari 95:5 kepada 25:75 di dalam masa 20 minit. Isipadu suntikan 5 μL adalah sesuai untuk larutan sampel berkepekatan 1 mg/ml. Suhu turus ditetapkan pada 35 °C dan kadar aliran fasa bergerak adalah pada 1.0 ml/min. Walaubagaimanapun, resolusi setiap kromatogram tidak sebaik dan sejitu apabila kaedah berlainan dibangunkan untuk setiap sampel secara individu. Kaedah ini telah telah menunjukkan keputusan yang positif apabila diuji ke atas sepuluh ekstrak lain dari famili tumbuhan yang sama. Sampel ujian tersebut adalah dari bahagian tumbuhan yang berlainan dan dari kawasan pensampelan yang berbeza.

 

Kata kunci:  dipterocarpaceae, Shorea spp., Dipterocarpus spp., kromatografi, kromatografi cecair prestasi tinggi

 

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