Malaysian Journal of Ana;ytical Sciences Vol 21 No 2 (2017): 304 - 311

DOI: https://doi.org/10.17576/mjas-2017-2102-05

 

 

 

THE CHEMICAL CONSTITUENTS FROM THE FRUITS OF Johannesteijsmannia altifrons AND ANTIBACTERIAL ACTIVITY

 

 (Komposisi Kimia Daripada Buah Johannesteijsmannia altifrons dan Aktiviti Antibakteria)

 

Noor Aziiraa Sabri¹, Wan Yaacob Wan Ahmad¹*, Nur Shafiqa Abdullah¹, Nazlina Ibrahim²

 

¹School of Chemical Sciences and Food Technology, Faculty of Science and Technology

²School of Biosciences and Biotechnology,Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

 

*Corresponding author: wanyaa@ukm.edu.my

 

 

Received: 10 October 2016; Accepted: 16 January 2017

 

 

Abstract

Isolation on the hexane extract of the fruits of Johannesteijsmannia altifrons by vacuum liquid and radial chromatography yielded three known compounds namely β-sitosterol, γ-taraxasterol and stigmasterol. Their structures were determined using one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy, fourier transform infra-red spectroscopy and mass spectrometry. All the isolated compounds were tested for their antibacterial activity using disc diffusion and minimum inhibitory concentration (MIC). All three compounds showed weak and moderate activities against all nine tested bacteria. This is the first report on the isolation of compounds from this species and on the antibacterial activity of γ-taraxasterol.

 

Keywords:  Johannesteijsmannia altifrons, β-sitosterol, γ-taraxasterol, stigmasterol, antibacterial

 

Abstrak

Pemencilan ke atas ekstrak heksana bagi buah Johannesteijsmannia altifrons dengan kromatografi cecair vakum dan radial menghasilkan tiga sebatian yang pernah ditemui bernama β-sitosterol, γ-taraksasterol dan stigmasterol. Strukturnya ditentukan dengan spektroskopi resonans magnet nukleus satu-dimensi dan dua-dimensi, spektroskopi infra-merah transformasi Fourier dan spektrosmetri jisim. Kesemua sebatian yang dipencilkan diuji aktiviti bakterianya dengan kaedah peresapan cakera dan kepekatan perencatan minimum (KPM). Kesemua tiga sebatian menunjukkan aktiviti yang lemah dan sederhana terhadap kesemua sembilan bakteria yang diuji. Ini merupakan laporan pertama mengenai pemencilan sebatian daripada spesies ini dan mengenai aktiviti antibakteria bagi γ-taraksasterol.

Kata kunci:  Johannesteijsmannia altifrons, β-sitosterol, γ-taraksasterol, stigmasterol, antibakteria

 

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