Malaysian Journal of Analytical Sciences Vol 21 No 3 (2017): 544 - 551

DOI: https://doi.org/10.17576/mjas-2017-2103-04

 

 

 

INTERACTION OF N,N’-BIS[4-[1-(2-HYDROXYETHOXY)] SALICYLIDENE]-PHENYLDIAMINE-NICKEL(II) AND COPPER(II) COMPLEXES WITH G-QUADRUPLEX DNA

 

(Interaksi Antara Kompleks N, N'-Bis [4- [1- (2-Hidroksietoksi)] Saliksalidin] -Fenildiamina-Nikel(II) dan Kuprum(II) Bersama DNA G-Kuadrupleks)

 

Roshatiara Shamsuddin, Muhammad Ameerullah Sahudin, Nur Hasyareeda Hassan, Nurul Huda Abd Karim*

 

School of Chemical Sciences and Food Technology, Faculty of Science and Technology,

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

 

*Corresponding author: nurulhuda@ukm.edu.my

 

 

Received: 29 December 2016; Accepted: 28 March 2017

 

 

Abstract

Binding studies of metal complexes with DNA has attracted many interests due to their importance in cancer therapy and molecular biology. In this study, N,N’-bis[4-[1-(2-hydroxyethoxy)]salicylidene]-phenyldiamine and its metal Ni(II) and Cu(II) complexes have been synthesized and characterized. The complexes were structurally characterized using standard spectroscopic 1D ¹H, 2D COSY, HMQC and ¹³C NMR spectroscopy, FTIR spectroscopy and ESI-MS spectrometry. The binding interaction between synthesized metal complexes and G-quadruplex DNA has been investigated using UV/Vis DNA titration study. Ni(II) and Cu(II) complexes bind strongly to G-quadruplex DNA (Kb = (5.42 ± 2.48) x 10⁶ M^-1 and (1.42 ± 2.48) x 10⁶ M^-1 respectively) via end stacking mode. Both complexes show selectivity for G-quadruplex DNA over CT- DNA by 2.15- and 1.82-fold, respectively.

 

Keywords:  metal complexes, DNA binding, G-quadruplex DNA

 

Abstrak

Kajian mengenai pengikatan kompleks logam bersama DNA telah menarik perhatian penyelidik disebabkan kepentingannya dalam perubatan terapi kanser dan molekular biologi. Dalam kajian ini, N, N'-bis [4-[1-(2-hidroksietoksi)] saliksalidin]-fenildiamina bersama logam Ni(II) dan Cu(II) kompleks telah berjaya disintesis dan dicirikan. Struktur kompleks telah dicirikan menggunakan spektroskopi piawai 1D ¹H, 2D COSY, HMQC dan spektroskopi ¹³C NMR, spektroskopi FTIR dan spektrometri ESI-MS. Pengikatan antara kompleks logam yang disintesis bersama DNA G-kuadrupleks telah dikaji dengan menggunakan kajian titratan DNA UV/Vis. Ni(II) dan Cu(II) kompleks memberikan nilai ikatan yang tinggi kepada DNA G-kuadrupleks ((5.42 ± 2.48) x 10⁶ M^-1 dan (1.42 ± 2.48) x 10⁶ M^-1) melalui mod penyusunan akhir. Ni(II) dan Cu(II) kompleks menunjukkan pemilihan pengikatan terhadap G-kuadrupleks DNA berbanding CT- DNA dengan 2.15 dan 1.82 kali ganda setiap satu.

 

Kata kunci:  kompleks logam, pengikatan DNA, DNA G-kuadrupleks

 

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