Sains Malaysiana 32: 147-155 (2003)                                                                                           Sains Hayat/

Life Science

Ketoksikan Logam Zink dan Kadmium

terhadap Anabaena flos-aquae

(Toxicity of zinc and cadmium on Anabaena flos-aquae)

Khairiah Jusoh, Carol Hoh Mui Ling & Nik Mazidah Nik Ahmad

Pusat Pengajian Sains Ssekitaran dan Sumber Alam

Fakulti Sains dan Teknologi

Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor D.E., Malaysia

Nik Marzuki Sidik

Pusat Pengajian BioSains dan Bioteknologi

 Fakulti Sains dan Teknologi

Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor D.E., Malaysia

Lee Yook Heng

Pusat Pengajian Sains Kimia dan Teknologi Makanan

Fakulti Sains dan Teknologi

Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor D.E., Malaysia

ABSTRAK

Kajian pengambilan logam zink (Zn) dan kadmium (Cd) oleh alga biru-hijau Anabena flos-aquae dan ketoksikan logam-logam ini telah dilakukan secara pengkulturan statik berasingan. A. flos-aquae didedahkan kepada kepekatan Zn dan Cd yang berbeza-beza. Kadar pertumbuhan A. flos-aquae ditentukan dengan kaedah spektrofotometri dan paras ketoksikan LD₅₀ 96 jam ditentukan melalui analisis Probit. Hasil kajian menunjukkan bahawa pengambilan kedua-dua logam ini oleh A flos-aquae mengikut corak yang serupa, iaitu apabila kepekatan logam meningkat, kuantiti logam diserap berkurangan. Walau bagaimanapun, pengambilan Zn oleh A. flos-aquae ialah tiga kali lebih tinggi berbanding Cd pada paras pendedahan 1.00 mg/L logam. lni menunjukkan Zn adalah lebih diperlukan oleh A. flos-aquae berbanding Cd. Pengambilan Cd yang rendah juga disebabkan oleh ketoksikan Cd yang lebih toksik. LD₅₀ 96 jam untuk Cd ialah 0.14 mg/L berbanding dengan LD₅₀ 96 jam Zn iaitu, 0.76 mg/L.

ABSTRACT

The uptake of the heavy metals zink (Zn) and cadmium (Cd) by the blue-green algae Anabena flos-aquae and their toxicity were studied using different static cultures. A. flos-aquae was exposed to different concentrations of Zn and Cd. The growth of the algae was monitored by spectrophotometric assay and the level of toxicity, LD₅₀ 96 h was measured by using Probit analysis. The study showed that uptake heavy metals by A. flos-aquae followed a similar trend but Zn was absorbed more than Cd. At the level of exposure of 1.00 mg/L for both metals, the absorption of Zn was three times higher than that of Cd. This indicates that Zn is required by A. flos-aquae as compared to Cd The low level of uptake of Cd may be attributed to the higher toxicity level of Cd LD₅₀ 96 h for Cd was 0.14 mg/L compared with that of Zn which was 0.76 mg/L.

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