Sains Malaysiana 39(6)(2010): 951–955

 

Physicochemical Characterization of Seed and Seed Oil of Jatropha curcas L. Collected from Bardoli (South Gujarat)

(Ciri-ciri Fizikokimia Biji dan Minyak Biji Jatropha curcas L. diambil dari Bardoli (Selatan Gujarat))

 

Bhavesh S. Nayak*

Department of Pharmacognosy

Shree Swaminarayan Sanskar Pharmacy College, Zundal, Gandhinagar, Gujarat, India

 

K.N. Patel

SAL Institute of Pharmacy, Ahmedabad, Gujarat, India

 

Received: 12 November 2009 / Accepted: 12 May 2010

 

ABSTRACT

 

The seed of Jatropha curcas was collected from the outskirts region of the Bardoli (Gujarat) and it was utilized for determination of seed characterization. The Jatropha curcas oil was extracted using light petroleum ether (60-80°C) by Soxhlet apparatus. The physicochemical properties of Jatropha curcas oil were evaluated. The result showed that the seeds consist of 46.31% (dry w/w) oil, moisture and volatilities (5.8% v/w) and protein content (22.50%). The physicochemical properties shows acid value (36.46), iodine value (106.00 mg/g) and saponification value (194.70 mg/g). The unsaponifiable matter was 1.02%. Negative Halphen test indicated the absence of cyclopropanoid acids in seed oil. GC analysis of J. curcas oil showed presence of palmitic acid (16.69%), stearic acid (7.67%), oleic acid (40. 39%) and linoleic acid (33.09%).

 

Keywords: Jatropha curcas; oil characterisation; physiochemical properties; seed characterisation

 

ABSTRAK

 

Biji Jatropha curcas telah dikumpulkan dari kawasan luar Bardoli (Gujarat) dan telah dicirikan. Minyak Jatropha curcas telah diekstrak menggunakan eter petroleum ringan (60-80oC) dan alat Soxhlet. Sifat fizikokimia minyak Jatropha curcas telah dinilai. Keputusan menunjukkan biji tersebut mengandungi 46.31% (w/w kering) minyak, kelembapan dan kemeruapan (5.8% v/w) dan protein (22.50%). Sifat fizikokimia menunjukkan nilai asid (36.46), nilai iodin (106.00 mg/g) dan nilai saponifikasi (194.70 mg/g). Jirim yang tak tersaponifikasi ialah 1.02%. Ujian Halphen negatif menunjukkan ketidakhadiran asid siklopropanoid di dalam minyqk biji ini. Analisis GC minyak J. curcas menunjukkan kewujudan asid palmitik (16.99%), asid steanik (7.67%), asid oleik (40.39%) dan asid linolik (33.09%).

 

Kata kunci: Ciri-ciri biji; ciri-ciri minyak; Jatropha curcas; sifat fizikokimia

 

REFERENCES

 

Adebowale, K.O. & Adedire, C.O. 2006. Chemical composition and insecticidal properties of the underutilized Jatropha curcas seed oil. African Journal of Biotechnology 5(10): 901-906.

Bailey, A.V., Pittman, R.A., Magne, F.C. & Skau, E.L. 1965. Methods for the determination of cyclopropenoid fatty acids: a spectrophotometric method for cotton seed oils based upon the Halphen-test reaction. J. Am. Oil Chem. Soc. 42: 422-424.

Carter, F.L. & Frampton, V.L. 1964. Adverse effects of cyclopropenoid fatty acids. Chem. Rev. 64: 497-525.

Deutschman, A.J. & Klaus I.S. 1960. Adverse effects of cyclopropenoid fatty acids. Anal. Chem. 32: 1809-1810.

Gerhard Knothe. 2002. Structure indices in FA chemistry. How relevant is the iodine value? J. Am. Oil Chem. Soc. 79(9): 847-854.

Gübitz, G.M., Mittlebach, M. & Trabi, M. 1999. Exploitation of the tropical oil seed plant Jatropha curcas. L. International of Biosource Technology 58: 77-82.

Halphen, G. 1897. Adverse effects of cyclopropenoid fatty acids. J. Pharm. 6: 390-392.

Kaushik, N., Kumar, K., Kumar, S., Kaushik, N. & Roy, S. 2007. Genetic variability and divergence studies in seed traits and oil content of Jatropha (Jatropha curcas L.) accessions. Biomass and Bioenergy 31: 497-502.

Link, W.E. 1975. Official and tentative methods of American oil and Chemists Society. 3rd edition. Champaign, USA: AOCS.

Makkar, H.P.S., Becker, K., Sporer, F. & Wink, M. 1997. Studies on nutritive potential and toxic constituents of different provenances of Jatropha curcas. J. Agr. Food Chem. 45: 3152-3157.

Martínez-Herrera, J., Siddhuraju, P., Francis, G., Dávila-Ortíz, G. & Becker K. 2006. Chemical composition, toxic/ antimetabolic constituents, and effects of different treatments on their levels, in four provenances of Jatropha curcas L. from Mexico. Food Chemistry 96: 80-89.

Morris L.J. & Hall, S.W. 1967. Fatty acid content of Pachira insignis, Bombacaceae. Chem. Indi. 32: 25-29.

Mukherjee P.K. 2002. Quality Control of Herbal Drugs. (1st ed) New Delhi: Business Horizon.

Nordby, H.E., Heywang, B.W., Kircher, H.W. & Kemmerer, A.R. 1962. Sterculic derivatives and pink egg formation. J. Am. Oil Chem. Soc. 39: 183-185.

Salimon Jumat & Abdullah Rozaini. 2008. Physicochemical properties of Malaysian Jatropha curcas seed oil. Sains Malaysiana. 37(4): 379-382.

Singhal, S.C. & Sekiya J. 2003. Modern Technology in the Oils and Fats Industry. (2nd ed.) New Delhi: AOSC-OTA13.

Vogel, A.I. 1975. A Text Book of Quantitative Inorganic Analysis 3rd Edition. London: Longman.

 

*Correspondence author; email: b_s_nayak@yahoo.co.in

 

 

 

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