Sains Malaysiana 43(12)(2014): 1895–1900

Antiproliferative Potential of Extracts from Kappaphycus Seaweeds on HeLa Cancer Cell Lines

(Potensi Antiproliferatif Ekstrak Rumpai Laut Kappaphycus ke atas Garisan Sel Kanser HeLa)

TIEK YING LAU¹, DELBORA FENNY VITTAL², CASSANDRA SZE YII CHEW¹

& WILSON THAU LYM YONG¹*

¹Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia

²School of Science and Technology, Universiti Malaysia Sabah, Jalan UMS

88400 Kota Kinabalu, Sabah, Malaysia

Received: 10 March 2013/Accepted: 24 April 2014

ABSTRACT

A review of the current literature indicates that natural seaweeds are an excellent source of bioactive compounds with antioxidant, antimicrobial and antitumor properties. In the present study, 90% methanolic, 70% acetonic and aqueous extracts from Kappaphycus alvarezii (strains Crocodile, Giant and Brown) and Kappaphycus striatum were used to inhibit the growth of HeLa cell lines. MTS assay was carried out to determine the proliferation of HeLa cells in the presence of different seaweed extracts. Both 500 μg/mL of aqueous and methanolic extracts from K. striatum demonstrated highest anti-proliferative activity against HeLa cells with cell growth inhibition of 53.5 and 43.7%, respectively. Treatment with the aqueous extracts from three strains of K. alvarezii did not show any growth inhibition against HeLa cell lines. The acetonic extract of Kappaphycus seaweeds exhibited a very poor cell growth inhibition with inhibitory activity observed under the treatment of 300 to 500 μg/mL of K. alvarezii strain Brown only. Further studies are suggested to identify and purify the specific anti-tumoral compounds for potential use in cancer therapy.

Keywords: Antiproliferation; growth inhibition; Kappaphycus alvarezii; Kappaphycus striatum

ABSTRAK

Suatu kajian kesusasteraan semasa menunjukkan bahawa rumpai laut semula jadi adalah sumber komponen bioaktif yang sangat baik dengan aktiviti antioksidan, antimikrobial dan antitumor. Dalam kajian ini, ekstrak metanol 90%, aseton 70% dan akueus daripada Kappaphycus alvarezii (strain Buaya, Giant dan Brown) dan Kappaphycus striatum telah digunakan untuk merencat pertumbuhan garisan sel HeLa. Asai MTS telah dijalankan untuk mengkaji pertumbuhan sel HeLa dalam kehadiran pelbagai ekstrak rumpai laut. Kedua-dua 500 μg/mL ekstrak akueus dan metanol daripada K. striatum menunjukkan aktiviti antiproliferasi yang tertinggi terhadap sel HeLa masing-masing dengan perencatan pertumbuhan sel sebanyak 53.5 dan 43.7%. Rawatan dengan ekstrak akueus daripada tiga strain K. alvarezii tidak menunjukkan perencatan pertumbuhan terhadap garisan sel HeLa. Ekstrak aseton daripada rumpai laut Kappaphycus mempamerkan perencatan pertumbuhan sel yang lemah dengan aktiviti perencatan hanya boleh diperhatikan dengan rawatan 300 hingga 500 μg/mL daripada K. alvarezii strain Brown sahaja. Kajian lanjutan adalah dicadangkan untuk mengenal pasti dan menulenkan komponen spesifik antitumor untuk pembangunan terapi kanser.

Kata kunci: Antiproliferasi; Kappaphycus alvarezii; Kappaphycus striatum; perencatan tumbuhan

REFERENCES

Abdel-Fattah, A.F., Hussein, M.M. & Salem, H.M. 1974. Studies of the purification and some properties of sargasan, a sulphated heterpolysaccharide from Sargassum linifolium. Carbohydrate Research 33: 9-17.

Abdulmalik, I.A., Sule, M.I., Musa, A.M., Yaro, A.H., Abdullahi, M.I., Abdulkadir, M.F. & Yusuf, H. 2011. Isolation of steroids from acetone extract of Ficus iteophylla. British Journal of Pharmacology and Toxicology 2: 270-272.

Albano, R.M., Pavao, M.S.G., Mourao, P.A.S. & Mulloy, B. 1990. Structural studies of a sulfated L-galactan from Styela plicata (Tunicate): Analysis of the Smith-degraded polysaccharide. Carbohydrate Research 208: 163-174.

Al-Haj, N.A., Mashan, N.I., Shamsudin, M.N., Mohamad, H., Vairappan, C.S. & Sekawi, Z. 2009. Antibacterial activity in marine algae Eucheuma denticulatum against Staphylococcus aureus and Streptococcus pyogenes. Research Journal of Biological Sciences 4: 519-524.

Bart, H.J. 2011. Extraction of natural products from plants - An introduction. In Industrial Scale Natural Products Extraction. 1st ed., edited by Bart, H.J. & Pilz, S. Weinheim: Wiley-VCH Verlag GmbH & Co. KGaA. pp. 1-25.

Berlinck, R.G.S., Ogawa, C.A., Almeida, A.M.P., Sanchez, M.A.A., Malpezzi, E.L.A., Costa, L.V., Hajdu, E. & de Freitas, J.C. 1996. Chemical and pharmacological characterization of halitoxin from Amphimedon viridis (Porifera) from the southeastern Brazilian coast. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology 115: 155-163.

de Sousa, A.P.A., Torres, M.R., Pessoa, C., de Moraes, M.O., Filho, F.D.R., Alves, A.P.N.N. & Costa-Lotufo, L.V. 2007. In vivo growth-inhibition of Sarcoma 180 tumor by alginates from brown seaweed Sargassum vulgare. Carbohydrate Polymers 69: 7-13.

Dias, P.F., Siqueira, J.M., Vendruscolo, L.F., de Jesus Neiva, T., Gagliardi, A.R., Maraschin, M. & Ribeiro-do-Valle, R.M. 2005. Antiangiogenic and antitumoral properties of a polysaccharide isolated from the seaweed Sargassum stenophyllum. Cancer Chemotherapy and Pharmacology 56: 436-446.

Duan, X.J., Zhang, W.W., Li, X.M. & Wang, B.G. 2006. Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata. Food Chemistry 95: 37-43.

Eloff, J.N. 1998. Which extractant should be used for the screening and isolation of antimicrobial components from plants? Journal of Ethnopharmacology 60: 1-8.

Itoh, H., Noda, H., Amano, H., Zhuang, C., Mizuno, T. & Ito, H. 1993. Antitumor activity and immunological properties of marine algal polysaccharides, especially fucoidan, prepared from Sargassum thunbergii of phaeophyceae. Anticancer Research 13: 2045-2052.

Jones, W.P. & Kingkorn, A.D. 2006. Extraction of plant secondary metabolites - Natural Products Isolation. In Methods in Biotechnology. vol. 20, 2nd ed., edited by Sarker, S.D., Latif, Z. & Gray, A.I. Totowa, New Jersey: Humana Press. pp. 323-351.

Kuda, T., Tsunekawa, M., Goto, H. & Araki, Y. 2005. Antioxidant properties of four edible algae harvested in the Noto Peninsula, Japan. Journal of Food Composition and Analysis 18: 625-633.

Lim, S.N., Cheung, P.C.K., Ooi, V.E.C. & Ang, P.O. 2002. Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum. Journal of Agricultural and Food Chemistry 50: 3862-3866.

Lindequist, U. & Schweder, T. 2001. Marine biotechnology. In Biotechnology, vol. 10, edited by Rehm, H.J. & Reed, G. Weinheim: Wiley-VCH. pp. 441-484.

Liu, S.C., Lin, J.T., Wang, C.K., Chen, H.Y. & Yang, D.J. 2009. Antioxidant properties of various solvent extracts from lychee (Litchi chinenesis Sonn.) flowers. Food Chemistry 114: 577- 581.

Lu, Y. & Yeap Foo, L. 1999. The polyphenol constituents of grape pomace. Food Chemistry 65: 1-8.

Luximon-Ramma, A., Bahorun, T., Crozier, A., Zbarsky, V., Datla, K.P., Dexter, D.T. & Aruoma, O.I. 2005. Characterization of the antioxidant functions of flavonoids and proanthocyanidins in Mauritian black teas. Food Research International 38: 357-367.

Ly, B.M., Buu, N.Q., Nhut, N.D., Thinh, P.D., Thi, T. & Van, T. 2005. Studies on fucoidan and its production from Vietnamese brown seaweeds. Asean Journal for Science and Technology Development (AJSTD) 22: 371-380.

Matanjun, P., Mohamed, S., Mustapha, N.M., Muhammad, K. & Ming, C.H. 2008. Antioxidant activities and phenolics content of eight species of seaweeds from north Borneo. Journal of Applied Phycology 20: 367-373.

Maruyama, H., Tamauchi, H., Hashimoto, M. & Nakano, T. 2003. Antitumor activity and immune response of Mekabu fucoidan extracted from Sporophyll of Undaria pinnatifida. In vivo 17: 245-249.

Matsuda, Y., Teruya, K., Matsuda, S., Nakano, A., Nishimoto, T., Ueno, M., Niho, A., Yamashita, M., Eto, H., Katakura, Y. & Shirahata, S. 2010. Anti-cancer effects of enzyme-digested fucoidan extract from seaweed Mozuku. Animal Cell Technology: Basic & Applied Aspects 16: 295-300.

Michio, F., Noriko, I., Ichiro, Y. & Terukazu, N. 1984. Purification and chemical and physical characterization of an antitumor polysaccharide from the brown seaweed Sargassum fulvellum. Carbohydrate Research 125: 97-106.

Newman, D.J., Cragg, G.M. & Snader, K.M. 2003. Natural products as source of new drugs over the period 1981-2002. Journal of Natural Products 66: 1022-1037.

Nishino, T., Yokoyama, G., Dobashi, K., Fujihara, M. & Nagumo, T. 1989. Isolation, purification and characterization of fucose-containing sulphated polysaccharides from the brown seaweed Ecklonia kurome and their blood-anticoagulant activities. Carbohydrate Research 186: 119-129.

Noda, H., Amano, H., Arashima, K. & Nisizawa, K. 1990. Antitumor activity of marine algae. Hydrobiologia 204-205: 577-584.

Nyenje, M. & Ndip, R.N. 2011. In-vitro antimicrobial activity of crude acetone extract of the stem bark of Combretum molle against selected bacterial pathogens. Journal of Medicinal Plants Research 5: 5315-5320.

Patel, S., Gheewala, N., Suthar, A. & Shah, A. 2009. In-vitro cytotoxicity activity of Solanum nigrum extract against Hela cell line and Vero cell line. International Journal of Pharmacy and Pharmaceutical Sciences 1: 38-46.

Shao, Y., Chin, C.K., Ho, C.T., Ma, W., Garrospm, S.A. & Huang, M.T. 1996. Anti-tumor activity of the crude saponins obtained from asparagus. Cancer Letters 104: 31-36.

Singh, A.K., Hussain, A., Srivastava, G.N., Misra, L.N., Gupta, M.M., Virmani, O.P., Popli, S.P. & Abraham, Z. 1992. Dictionary of Indian Medicinal Plants. Lucknow, India: Central Institute of Medicinal and Aromatic Plants (CIMAP).

Smit, A.J. 2004. Medicinal and pharmaceutical uses of seaweed natural products: A review. Journal of Applied Phycology 16: 245-262.

Sun, S.G. 2002. Reply to the comments on the paper by Zheng M.S. and Sun S.G. entitled ‘In situ FTIR spectroscopic studies of CO adsorption on electrodes with nanometer-scale thin films of ruthenium in sulphuric acid solutions’ by Pecharromán, C., Cuesta, A. & Gutiérrez, C. Journal of Electroanalytical Chemistry 529: 155-158.

Takamatsu, S., Hodges, T.W., Rajbhandari, I., Gerwick, W.H., Hamann, M.T. & Nagle, G. 2003. Marine natural products as novel antioxidant prototypes. Journal of Natural Products 66: 605-608.

Usui, T., Asari, K. & Mizuno, T. 1980. Isolation of highly purified “Fucoidan” from Eisenia bicylclis and its anticoagulant and antitumor activities. Agricultural and Biological Chemistry 44: 1965-1966.

van Slambrouck, S., Daniels, A.L., Hooten, C.J., Brock, S.L., Jenkins, A.R., Ogasawara, M.A., Baker, J.M., Adkins, G., Elias, E.M., Agustin, V.J., Constantine, S.R., Pullin, M.J., Shors, S.T., Kornienko, A. & Steelant, W.F.A. 2007. Effect of crude aqueous medicinal plant extracts on growth and invasion of breast cancer cells. Oncology Reports 17: 1487- 1492.

Xu, J.P., Xu, R.S. & Li, X.Y. 1992. Four new cycloartane saponins from Curculigo orchioides. Planta Medica 58: 208-210.

Xu, N., Fan, X., Yan, X. & Tseng, C.K. 2004. Screening marine algae from China for their antitumor activities. Journal of Applied Phycology 16: 451-456.

Yamamoto, I., Takahashi, M., Suzuki, T., Seino, H. & Mori, H. 1984. Antitumor effect of seaweeds. IV. Enhancement of antitumor activity by sulfation of a crude fucoidan fraction from Sargassum kjellmanianum. The Japanese Journal of Experimental Medicine 54: 143-151.

Yuan, Y.V. & Walsh, N.A. 2006. Antioxidant and antiproliferative activities of extracts from a variety of edible seaweeds. Food and Chemical Toxicology 44: 1144-1150.

Zandi, K., Tajbakhsh, S., Nabipour, I., Rastian, Z., Yousefi, F., Sharafiah, S. & Sartavi, K. 2010. In vitro antitumor activity of Gracilaria corticata (a red alga) against Jurkat and molt-4 human cancer cell lines. African Journal of Biotechnology 9: 6787-6790.

Zhuang, C., Itoh, H., Mizuno, T. & Ito, H. 1995. Antitumor active fucoidan from the brown seaweed, umitoranoo (Sargassum thunbergii). Bioscience, Biotechnology, and Biochemistry 59: 563-567.

*Corresponding author; email: wilsonyg@ums.edu.my