Sains Malaysiana 44(9)(2015): 1269–1273

Antibacterial Activities of Selected Seaweed and Seagrass from Port Dickson Coastal Water against different Aquaculture Pathogens

(Aktiviti Antibakteria daripada Rumpai Laut dan Rumput Laut Terpilih di Pesisiran Port Dickson terhadap Patogen Akuakultur Berbeza)

F.M.I. NATRAH^1,2*, Z. MUTA HARAH^1,3, B. JAPAR SIDIK², N.M.S. IZZATUL¹ & A. SYAHIDAH¹

¹Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia

43400 Serdang, Selangor Darul Ehsan, Malaysia

²Faculty of Agriculture and Food Sciences, Universiti Putra Malaysia, Bintulu Sarawak Campus

97008 Bintulu, Sarawak Bumi Kenyalang, Malaysia

³Laboratory of Marine Biotechnology, Institute Bioscience, Universiti Putra Malaysia

43400 Serdang, Selangor Darul Ehsan, Malaysia

Received: 23 May 2014/Accepted: 15 May 2015

ABSTRACT

Eight seaweed species in Teluk Kemang and three seagrass species in Teluk Pelanduk, Port Dickson, respectively, were screened for antibacterial activities. The antibacterial activities were screened using disc diffusion test, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against six aquacultural pathogens strains Aeromonas hydrophila ATCC35654, Vibrio harveyi BB120, Vibrio harveyi ATCC14126, Vibrio alginolyticus ATCC17749, Vibrio parahaemolyticus ATCC17803 and Vibrio anguillarum ATCC43313. The results showed that among all the pathogens, seaweed Padina minor and seagrass Thalassia hemprichii had the strongest antibacterial activity against Vibrio harveyi BB120 and Vibrio harveyi ATCC14126, respectively. The lowest values for minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were obtained from Padina minor against V. harveyi BB120 and Thalassia hemprichii against V. harveyi ATCC14126, respectively. The findings suggested that seaweed and seagrass in Port Dickson coastal water have the potential to prevent bacterial diseases particularly in aquaculture.

Keywords: Antibacteria; aquaculture pathogens; Port Dickson; seagrass; seaweed

ABSTRAK

Lapan rumpai laut dari Teluk Kemang dan tiga rumput laut dari Teluk Pelanduk Port Dickson telah disaring untuk aktiviti antibakteria. Aktiviti antibakteria telah disaring dengan menggunakan ujian resapan disk, pemekatan resapan minimum (MIC) dan pemekatan bakteriasid minimum (MBC) terhadap enam patogen akuakultur Aeromonas hydrophila ATCC35654, Vibrio harveyi BB120, Vibrio harveyi ATCC14126, Vibrio alginolyticus ATCC17749, Vibrio parahaemolyticus ATCC17803 dan Vibrio anguillarum ATCC43313. Hasil uji kaji menunjukkan daripada kesemua patogen, rumpai laut Padina minor dan rumput laut Thalassia hemprichii menunjukkan aktiviti antibakteria yang paling tinggi terhadap Vibrio harveyi BB120 dan Vibrio harveyi ATCC14126. Nilai paling rendah bagi asai pemekatan resapan minimum (MIC) dan pemekatan bakteriasid minimum (MBC) diperoleh daripada Padina minor terhadap V. harveyi BB120 dan Thalassia hemprichii terhadap V. harveyi ATCC14126. Hasil kajian ini mencadangkan rumpai laut dan rumput laut dari pesisiran pantai Port Dickson berpotensi untuk mencegah penyakit bakteria terutamanya dalam akuakultur.

Kata kunci: Antibakteria; patogen akuakultur; Port Dickson; rumpai laut; rumput laut

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*Corresponding author; email: natrah@upm.edu.my