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Sains Malaysiana 50(8)(2021): 2319-2328
http://doi.org/10.17576/jsm-2021-5008-15
Comparing the Effects of Alpha-Tocopherol and Tocotrienol Isomers on
Osteoblasts hFOB 1.19 Cultured on Bovine Bone
Scaffold
(Perbandingan Kesan Isomer Alfa-Tokoferol dan Tokotrienol pada Osteoblas hFOB 1.19
yang Dikultur atas Perancah Tulang Bovin)
1Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan
Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Federal Territory, Malaysia
2Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan
Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Federal Territory, Malaysia
3Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan
Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Federal Territory, Malaysia
Received:
3 April 2020/Accepted: 9 December 2020
ABSTRACT
Tocotrienol mixtures have been shown to exert anabolic actions on the
skeletal system in animal studies, but it is unclear which tocotrienol isomer
shows the most prominent effects. This study aims to investigate the most
active tocotrienol isomers using hFOB 1.19 human
osteoblasts cultured on a bovine bone scaffold. The bovine trabecular bone was
sectioned, demineralised and freeze-dried to form the scaffold. hFOB 1.19 osteoblasts were cultured on the bone scaffolds
in humidified condition at 37 °C and 5% carbon dioxide with vitamin E isomers
(alpha-, beta-, gamma-, delta-tocotrienol and alpha-tocopherol). The cell
differentiation capacity of tocotrienol isomers was investigated through
morphological observation, alkaline phosphatase (ALP) activity and osteocalcin
expression. Changes in the bone scaffolds were determined using
histomorphometry methods. Osteoblast culture treated with gamma- and
delta-tocotrienols showed a significant increase in ALP activity and
osteocalcin expression. Bone structural histomorphometry analysis showed that
bone scaffolds treated with gamma- and delta-tocotrienol showed significant
increases in bone volume and trabecular thickness. In conclusion, gamma- and
delta-tocotrienol show the most prominent bone anabolic effects by increasing
osteoblast differentiation and enhancing bone microstructure.
Keywords:
Bone; osteoblast; osteoporosis; tocopherol; tocotrienol
ABSTRAK
Campuran tokotrienol telah terbukti memberi kesan anabolik kepada sistem rangka di dalam kajian haiwan, tetapi isomer tokotrienol yang paling berkesan belum dikenal pasti.
Kajian ini bertujuan mengkaji isomer tokotrienol yang
paling aktif dengan menggunakan sel osteoblas manusia hFOB 1.19 yang dikultur atas tulang perancah lembu. Tulang trabekular lembu dipotong dan dikering-beku untuk membentuk perancah. Sel osteoblas hFOB 1.19 dikulturkan di atas perancah tulang dalam kelembapan 37 °C dan 5% karbon dioksida bersama isomer vitamin E
(alfa-tokotrienol, beta-tokotrienol, gamma-tokotrienol, delta-tokotrienol dan alfa-tokoferol). Kapasiti pembezaan sel isomer tokotrienol dikaji melalui pemerhatian morfologi, aktiviti alkalin phosphatase (ALP) dan ekspresi osteokalsin. Perubahan struktur tulang ditentukan dengan kaedah histomorfometri. Sel osteoblas yag dirawat dengan gamma- dan
delta-tokotrienol menunjukkan peningkatan aktiviti ALP
dan ekspresi osteokalsin yang ketara. Tulang perancah yang dirawat dengan gamma-tokotrienol dan
delta-tokotrienol mempunyai peningkatan isi padu serta ketebalan trabekular yang ketara melalui analisis histomorfometri. Secara kesimpulannya, gamma- dan delta-tokotrienol menunjukkan kesan anabolik tulang yang paling ketara dengan meningkatkan pembezaan osteoblas dan struktur seni tulang.
Kata kunci: Osteoblas;
osteoporosis; tokoferol; tokotrienol; tulang
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*Corresponding
author; email: imasoel@ppukm.ukm.edu.my
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