Sains Malaysiana 43(7)(2014): 1031–1036

 

Ameliorative Effect of Palm Oil Tocotrienol Rich Fraction on

Brain Oxidative Stress in Fenitrothion-administered Rats

(Kesan Pemulihan Pecahan Kaya Tokotrienol Minyak Kelapa Sawit terhadap Tekanan Oksidatif Otak Tikus Diadministrasi Fenitrotion)

SITI BALKIS BUDIN*, IZATUS SHIMA TAIB, PUTRI AYU JAYUSMAN, HUI HUI CHIANG, ANAND RAMALINGAM, AHMAD ROHI GHAZALI & JAMALUDIN MOHAMED

Program of Biomedical Science, School of Diagnostic and Applied Health Sciences

Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz

50300 Kuala Lumpur, Malaysia

 

Diserahkan: 6 Disember 2012/Diterima: 27 September 2013

 

 

ABSTRACT

Fenitrothion (FNT) usage has received much attention for its potential to promote free radicals generation and interfere with antioxidant defense system. The aim of the present study was to investigate the effect of palm oil tocotrienol rich fraction (TRF) supplementation on oxidative stress and histological changes in rat brain induced by FNT. A total of 32 male Sprague Dawley rats divided into four groups: control group which received corn oil; TRF group was received palm oil TRF (200 mg/kg bw); FNT group administered with FNT (20 mg/kg bw) and TRF+FNT group pretreated with palm oil TRF (200 mg/kg bw) 30 min prior to administration of FNT (20 mg/kg bw). FNT and TRF were dissolved in corn oil and all supplementations were given by oral gavage once daily for 28 days. After four weeks of supplementation, TRF+FNT rats had significantly lower malondialdehyde (MDA) content and superoxide dismutase (SOD) activity but higher reduced glutathione (GSH) level and total protein level compared to FNT rats (p<0.05). However, protein carbonyl (PC) level was insignificantly lower for TRF+FNT group compared to FNT group. In conclusion, this study suggested that palm oil TRF was effective in preventing brain damage in rats.

 

Keywords: Fenitrothion; oxidative damage; palm oil tocotrienol rich fraction

 

ABSTRAK

Kegunaan fenitrothion (FNT) telah mendapat perhatian ramai kerana insektisida ini berupaya untuk mengaruh penjanaan radikal bebas dan menganggu sistem pertahanan antioksidan. Tujuan kajian ini adalah untuk mengetahui kesan pecahan kaya tokotrienol (TRF) minyak sawit terhadap tekanan oksidatif dan perubahan histologi otak tikus teraruh FNT. Tiga puluh dua ekor tikusSprague Dawley jantan telah dibahagikan kepada empat kumpulan: kumpulan kawalan yang menerima minyak jagung; kumpulan TRF yang menerima TRF (200 mg/kg bw); kumpulan FNT yang diadministrasi FNT (20 mg/kg bw) dan kumpulan TRF+FNT yang diberi TRF (200 mg/kg bw) 30 min selepas diadministrasi FNT (20 mg/kg bw). FNT dan TRF telah dilarutkan dalam minyak jagung dan semua suplemen telah diberi secara oral untuk 28 hari. Selepas empat minggu suplementasi, tikus TRF+FNT menunjukkan penurunan aras malonaldehid (MDA) dan aktiviti superoksida dismutase (SOD) tetapi aras glutation terturun (GSH) serta protein jumlah lebih tinggi berbanding tikus FNT (p<0.05). Namun, aras karbonil protein (PC) kumpulan TRF+FNT adalah rendah berbanding kumpulan FNT secara tidak signifikan. Kesimpulannya, kajian ini mencadangkan bahawa TRF minyak sawit dapat mengurangkan tekanan oksidatif dan mengelakkan kerosakan otak tikus aruhan FNT secara berkesan.

 

Kata kunci: Fenitrotion; kerosakan oksidatif; pecahan kaya tokotrienol minyak sawit

 

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*Pengarang untuk surat-menyurat; email: balkis6466@yahoo.com.my

 

 

 

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