Sains Malaysiana 45(2)(2016): 237–245

Troubleshooting and Maintenance of High-Performance Liquid Chromatography during

Herbicide Analysis: An Overview

(Merumus Masalah dan Penyelenggaraan Kromatografi Cecair Berprestasi Tinggi semasa

Analisis Herbisid: Suatu Gambaran Keseluruhan)

Tayeb, M.A.¹,* Ismail, B.S¹., Khairiatul-Mardiana, J.¹ & GOH CHOO TA²

¹School of Environmental and Natural Resource Sciences, Faculty of Science and Technology,

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia

²Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia
43600 Bangi, Selangor Darul Ehsan, Malaysia

Diserahkan: 27 Ogos 2014/Accepted: 5 Ogos 2015

ABSTRACT

Glufosinate ammonium or ammonium salt (ammonium-(2RS)-2-amino-4- (methylphosphinato) butyric acid; C5H15N2O4P) is a commonly used polar herbicide in Malaysia and present in a variety of environmental waters at the sub-ppb level. Thus, glufosinate ammonium is analyzed in soil and water using high-performance liquid chromatography (HPLC), which is a complex yet the most powerful analysis tool. HPLC is tremendously sensitive and highly automated and HPLC instrumentation and machinery have improved over the years. However, typical problems are still encountered. HPLC users and advanced learners require help in identifying, separating and correcting typical problems. All HPLC systems consist of similar basic components. Although it is a modular system, trouble can occur in each component and change the overall performance. Resolving these problems may be expensive. This review describes the different aspects of HPLC, particularly troubleshooting, common problems and easy guidelines for maintenance.

Keywords: Glufosinate ammonium; HPLC; maintenance; troubleshoot

ABSTRAK

Ammonium glufosinate atau garam ammonium (ammonium-(2RS)-2-amino-4-(methylphosphinato) asid butirik; C5H15N2O4P) adalah herbisid berkutub yang biasa digunakan di Malaysia dan hadir di dalam persekitaran air pada kepekatan sub-ppb. Oleh itu, ammonium glufosinate dianalisis di dalam tanah dan air dengan menggunakan kromatografi cecair berprestasi tinggi (HPLC) yang merupakan alat yang kompleks namun sangat jitu dalam menganalisis herbisid ini. HPLC sangat sensitif dan automatik serta prestasinya sering dipertingkatkan dari semasa ke semasa. Walau bagaimanapun, terdapat beberapa masalah sering dihadapi pengguna. Pengguna dan pengendali HPLC pada peringkat lebih tinggi memerlukan bantuan dalam proses pengenalpastian, pengasingan dan membetulkan permasalahan yang sering berlaku. Kesemua sistem HPLC terdiri daripada komponen asas yang sama. Walaupun ia adalah satu sistem yang modular, masalah boleh berlaku dalam setiap komponen dan mengubah keseluruhan prestasi. Menyelesaikan masalah ini mungkin memerlukan kos yang tinggi. Maka, ulasan ini menerangkan pelbagai aspek HPLC, terutamanya penyelesaian masalah HPLC, masalah yang sering berlaku dan garis panduan yang mudah untuk penyelenggaraan.

Kata kunci: Ammonium glufosinate; HPLC; merumus masalah; penyelenggaraan

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*Pengarang untuk surat-menyurat; email: atayeb91@yahoo.com