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Sains Malaysiana 45(4)(2016): 609–613 Aktiviti Anti-Acanthamoeba
oleh Disinfektan Kanta Sentuh Pelbagai Guna terhadap Sista
Acanthamoeba Pencilan Klinikal dan Persekitaran (Anti-Acanthamoeba
Activity of Multi-purpose Contact Lens Disinfecting Solutions
against Acanthamoeba
Cysts of Clinical and Environmental Isolates) MOHAMED
KAMEL
ABD
GHANI1*,
SYAMIMI
OMAR1,
ANISAH
NORDIN2,
YUSOF
SUBOH2,
NORAINA
AB
RAHIM2,
WAN
OMAR
ABDULLAH3
& NORAZAH AHMAD4 1Program Sains Bioperubatan, Fakulti
Sains Kesihatan, Universiti Kebangsaan Malaysia Jalan
Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia 2Jabatan Parasitologi Perubatan,
Fakulti Perubatan, Universiti Kebangsaan Malaysia Jalan
Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia 3Jabatan
Mikrobiologi dan Parasitologi Perubatan, Fakulti Perubatan dan
Sains Kesihatan Universiti
Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia 4Unit
Bakteriologi, Institut Penyelidikan Perubatan, Jalan Pahang, 50588
Kuala Lumpur Malaysia Diserahkan:
21 Ogos 2014/Diterima: 26 Oktober 2015 ABSTRAK Disinfeksi kanta sentuh
yang berkesan adalah sangat penting sebagai langkah pencegahan
keratitis Acanthamoeba (AK). Oleh yang demikian, kajian
ini dijalankan untuk menilai keberkesanan larutan disinfektan
kanta sentuh pelbagai guna terhadap sista Acanthamoeba dengan
menggunakan empat pencilan, dua pencilan klinikal; HS 5, HTH 73,
dan dua lagi pencilan persekitaran; TTA 1 dan TKA 14.
Lima jenis larutan disinfektan pelbagai guna; Complete®,
Renu® fresh™, RevitaLens OcuTec®,
Opti-Free® Express® dan Solo Care Aqua® telah
diuji ke atas pencilan Acanthamoeba tersebut. Masa rendaman
adalah berdasarkan masa yang disyorkan oleh pengeluar (4 jam dan
6 jam), 8 jam dan 24 jam. Setiap campuran sista dan larutan disinfektan
kanta sentuh dipindahkan ke atas agar tanpa nutrien yang dilapisi
Escherichia coli. Plat agar diperiksa di bawah mikroskop
songsang setiap hari sehingga hari ke-14 untuk melihat kehadiran
trofozoit. Kelima-lima larutan disinfektan pelbagai guna kanta
sentuh tidak efektif membunuh kesemua pencilan sista Acanthamoeba
yang diuji. Ini menunjukkan bahawa kebanyakan larutan disinfektan
pelbagai guna kanta sentuh tidak mempunyai aktiviti anti-Acanthamoeba
yang sangat diperlukan bagi mencegah jangkitan keratitis Acanthamoeba
dalam kalangan pengguna kanta sentuh. Kata kunci: Acanthamoeba; keberkesanan; larutan disinfektan pelbagai guna
kanta sentuh; Malaysia ABSTRACT Effective disinfection
of contact lenses is of paramount importance in the prevention
of Acanthamoeba (AK). Therefore, this study was
undertaken to evaluate the effectiveness of the multi-purpose
contact lens disinfecting solutions against Acanthamoeba cysts
of four isolates comprising two clinical isolates; HS 5, HTH 73,
and two environmental isolates; TTA 1 and TKA 14.
Five multi-purpose contact lens disinfecting solutions; Complete®,
Renu® fresh™, RevitaLens OcuTec®, Opti-Free® Express® and
Solo Care Aqua® were tested on cyst of the four
Acanthamoeba isolates. The soaking times were based on
manufactures recommendations (4 and 6 h), 8 h and 24 h. Each mixture
of the cysts and disinfecting solution was transferred onto non-nutrient
agar seeded with Escherichia coli. The agar plates were
examined under inverted microscope daily until day 14 to detect
the presence of Acanthamoeba trophozoites. All 5 multi-purpose
contact lens disinfecting solutions tested were ineffective at
killing all Acanthamoeba cyst isolates tested. This indicates
that most multi-purpose contact lens disinfecting solutions do
not have anti-Acanthamoeba activity necessary to prevent
keratitis amongst the contact lens wearers. Keywords: Acanthamoeba; effectiveness; Malaysia; multi-purpose contact lens
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Interdiciplinary Perspectives on Infectious Diseases 2009:
Article ID 251406. *Pengarang untuk surat-menyurat;
email: mohamedkamela@yahoo.com
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