Sains Malaysiana 46(9)(2017): 1369–1377

http://dx.doi.org/10.17576/jsm-2017-4609-03

Fluorescence Quenching Reaction of Chlorophyll a by Tris(acetylacetonate)Iron(III) in Various Solvents

(Tindak Balas Pemelindapan Kependarfluoran Klorofil a oleh Tris(asetilacetonat)Iron(III) dalam Pelbagai Pelarut)

NARARAK LEESAKUL¹*, DAWEENA MASEN² & GUENTER GRAMPP³

¹Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90110, Thailand

²Public Health Program, Faculty of Science and Technology, Southern College of Technology

Nakorn Si Thammarat 80110, Thailand

³Institute of Physical and Theoretical Chemistry, Graz University of Technology, Graz, A-8010

Austria

Diserahkan: 31 Ogos 2016/Diterima: 17 Januari 2017

ABSTRACT

Chlorophyll a is known as the prevailing light absorbing pigment giving a strong absorption and fluorescence emission in visible region. Quenching reactions of the chlorophyll a fluorescence by Fe(acac)₃ were precisely investigated in various organic solvents which are benzene toluene, ethanol, methanol, dmf, dmso and acetonitrile. Electron transfer performance of chlorophyll a by Fe(acac)₃ was investigated from oxidative quenching reaction. Herein, the simplified Rehm-Weller relationship was used to calculate the free energy change of the photo-induced electron transfer reaction. Emission intensity decreased when the concentration of Fe(acac)₃ quencher was increased. Non-linear Stern-Volmer plots are found to be affected by inner filter effect more than the ground state complex formation. Rate of quenching reactions (kq) were determined from the Stern-Volmer equation with corrected inner filter effect. The rates of quenching reactions occurred faster in high viscous solvents.

Keywords: Chlorophyll a; oxidative quenching reaction; Stern-Volmer plotting

ABSTRAK

Klorofil a dikenali sebagai pigmen penyerap cahaya yang memberikan penyerapan yang kuat dan pelepasan kependarfluoran kawasan yang boleh dilihat. Tindak balas pemelindapan klorofil a oleh Fe(acac)₃ dikaji secara tepat dalam pelbagai pelarut organik seperti benzena toluen, etanol, metanol, dmf, dmso dan asetonitril. Prestasi pemindahan elektron klorofil a oleh Fe(acac)₃ telah dikaji daripada tindak balas pemelindapan oksidatif. Di sini, hubungan Rehm-Weller dipermudah telah digunakan untuk mengira perubahan tenaga bebas tindak balas pemindahan elektron teraruh-foto. Keamatan pelepasan menurun apabila kepekatan pelindap Fe(acac)₃ meningkat. Plot Stern-Volmer tak linear terjejas oleh kesan turas dalaman lebih daripada pembentukan keadaan asas yang kompleks. Kadar tindak balas pemelindapan (kq) telah ditentukan daripada persamaan Stern-Volmer kesan turas dalaman diperbetulkan. Kadar tindak balas pemelindapan berlaku dengan lebih cepat dalam pelarut likat yang tinggi.

Kata kunci: Klorofil a; plot Stern-Volmer; tindak balas sepuhlindap oksidatif

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*Pengarang untuk surat-menyurat; email: nararak.le@psu.ac.th