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Sains Malaysiana 47(10)(2018):
2401–2409 http://dx.doi.org/10.17576/jsm-2018-4710-16
Kesan Polifenol Minyak Kelapa Dara ke atas Aktiviti Enzim Antioksidan dan Lipid Peroksida Pada Sel Osteoblas (Virgin Coconut Oil Polyphenol Effects on Antioxidant Enzyme Activities and Lipid Peroxidation on Osteoblast
Cell) ZIL HAYATULLINA,
NORAZLINA
MOHAMED,
IMA-NIRWANA
SOELAIMAN
& NORLIZA MUHAMMAD* Jabatan Farmakologi,
Fakulti Perubatan,
Universiti Kebangsaan Malaysia,
50300
Kuala Lumpur, Wilayah Persekutuan, Malaysia Diserahkan: 3
April 2018/Diterima: 5 Jun 2018 ABSTRAK Osteoporosis
dikaitkan dengan
radikal bebas dan
tekanan oksidatif. Kandungan polifenol yang tinggi
dalam minyak
kelapa dara (VCO)
yang bertindak sebagai
antioksidan mampu menghalang tekanan oksidatif seterusnya mencegah osteoporosis. Uji kaji
in vitro ini dijalankan untuk mengkaji mekanisme kesan perlindungan polifenol ekstrak daripada VCO (PF)
ke atas sel
selanjar pre-osteoblas
mencit (MC3T3-E1) teraruh
tekanan oksidatif.
Untuk mengkaji viabiliti kesan perlindungan polifenol terhadap MC3T3-E1 daripada
toksisiti hidrogen
peroksida (H2O2),
sel MC3T3-E1 dirawat dengan beberapa dos polifenol selama 24 jam selepas dieram dengan 250 μM (IC50) H2O2 selama 1 jam. Mekanisme perlindungan dikaji dengan mengukur aras malondialdehid
(MDA)
serta aktiviti
enzim antioksidan iaitu superoksida dismutase (SOD),
glutation peroksidase
(GPx) dan katalase
(CAT).
Keputusan kajian
menunjukkan bahawa kepekatan perencatan median (IC50)
H2O2 pada 1 jam adalah 250 μM. Ujian viabiliti sel
mendapati kesemua dos polifenol dapat melindungi sel MC3T3-E1
daripada toksisiti
H2O2.
Dos 1 dan 0.01 μM
polifenol dapat meningkatkan aras
GPx dan CAT,
manakala dos 0.01 dan 0.0001 μM dapat meningkatkan
aras SOD. Sementara
itu, hanya
dos 0.01 dan 0.001 μM sahaja yang dapat menghalang peningkatan aras MDA.
Kesimpulan yang
dapat dibuat di dalam kajian ini
adalah polifenol
daripada minyak kelapa dara mencegah
lipid peroksida pada
sel MC3T3-E1 yang telah
diaruh dengan
H2O2 dengan meningkatkan aktiviti enzim antioksidan. Kata kunci: Osteoporosis; polifenol;
sel MC3T3-E1; tekanan oksidatif; VCO ABSTRACT Osteoporosis is associated
with free radicals and oxidative stress. The high polyphenol contents
in virgin coconut oil (VCO) can prevent oxidative stress
and consequently osteoporosis. This in vitro study was conducted in order to determine
the mechanism of the protective effects of polyphenol extracted
from VCO
on murine osteoblast-like cell line (MC3T3-E1)
which was induced with oxidative stress. To study the protective
effects on viability of MC3T3-E1 cells exposed
to hydrogen peroxide (H2O2)
toxicity, MC3T3-E1 cells were post-treated
with different doses of polyphenol for 24 h after 1 h incubation
with 250 μM of H2O2 (IC50).
The protective mechanism was studied by measuring melondialdehyde
(MDA)
levels as well as antioxidant enzymes activities which are superoxide
dismutase (SOD),
glutathione peroxidase (GPx) and catalase
(CAT).
The results showed that the median inhibitary
concentration (IC50) for H2O2 after
1 h was 250 μM. Cell viability
tests showed that all the doses of polyphenol were able to protect
MC3T3-E1 cells from H2O2 toxicity.
The dose of 1 and 0.01 μM of polyphenol
increased the level of GPx and CAT while
the dose of 0.01 and 0.0001 μM
increased the level of SOD. Meanwhile, the 0.0001 μM
of PF prevented the MDA elevation. In conclucion,
the polyphenol components in virgin coconut oil prevent lipid
peroxidation in MC3T3-E1 cells induced with
H2O2 by
increasing the endogenous antioxidant enzymes. Keywords: MC3T3-E1 cells; osteoporosis; oxidative
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