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http://dx.doi.org/10.17576/jsm-2019-4809-01
Haematological and
Biochemical Responses of Juvenile Malabar Blood Snapper (Lutjanus
malabaricus Bloch & Schneider, 1801) Exposed to Different Rearing
Temperatures and Diets
(Tindak Balas Hematologi
dan Biokimia Ikan Merah Juvenil Tanda Hitam (Lutjanus malabaricus
Bloch & Schneider, 1801) Terdedah kepada Suhu dan Diet Penternakan
Berbeza)
SABUJ KANTI MAZUMDER1,5, SVEINUNG FIVELSTAD2, MAZLAN ABD. GHAFFAR3 & SIMON KUMAR DAS1,4*
1Centre for Ecosystem
Management and Natural Resources, Faculty of Science and Technology, Universiti
Kebangsaan Malaysia, 43600 UKM Bangi Selangor, Darul Ehsan, Malaysia
2Department of
Biochemical Laboratory Sciences and Chemical Engineering, Western Norway
University of Applied Sciences, Bergen, Norway
3Institute of
Oceanography and Environment, University Malaysia Terengganu, 21030 Kuala
Nerus, Terengganu Darul Iman, Malaysia
4Marine Ecosystem
Research Center, Faculty of Science and Technology, Universiti Kebangsaan
Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
5Department of Genetics
and Fish Breeding, Faculty of Fisheries, Bangabandhu Sheikh Mujibur Rahman
Agricultural University, Gazipur-1706, Bangladesh
Diserahkan:
29 Oktober 2018/Diterima: 14 Jun 2019
ABSTRACT
This study is conducted
to examine the impacts of different rearing temperature and diet variation on
haematological and serum biochemical parameters on the juvenile Lutjanus malabaricus, a commercially important fish species in
Malaysia. Fish (16-18 cm) were divided into eight treatments (4 temperatures ×
2 diets) each with three replicates of five juveniles each. Fish were subjected
to four different rearing temperatures (22, 26, 30 and 34°C) and fed with a
commercial pellet and a natural shrimp diet. After 30 days of experimental
period, blood samples were collected and transferred into two different tubes
from each sample: One containing EDTA as an anticoagulant agent
and the other without EDTA for serum collection. All the
haematological and biochemical parameters in all the treatments were within the
normal range. Changing temperature and diet led to a significant change (p<0.05)
in the total length (TL), body weight (BW),
red blood cell (RBC), haemoglobin (HB),
haematocrit (HC), thrombocyte (TC) and white blood cell (WBC)
but the change in mean corpuscular volume (MCV),
mean cell haemoglobin (MCH) while mean cell haemoglobin
concentration (MCHC) were not significant (p>0.05).
The fish reared at 26°C and 30°C had significantly higher values than those
reared at 22°C and 34°C (p<0.05). Total protein (TP),
albumin (ALB), albumin/globin ration (A/G ratio), alkaline
phosphatase (AP), aspartate aminotransferase (AST)
and alanine transaminase (ALT) level were also significantly
increased at 30°C and 26°C compared to that of 34°C and 22°C. Globulin (GLB)
level showed the opposite trend. In all the cases the performances were higher
in shrimp diet than pellet diet. It is suggested that these physiological
parameters can be conveniently employed as health monitoring tools in fish
culture practices.
Keywords: Diet; fish;
haematology; serum biochemistry; temperature
ABSTRAK
Kajian ini dijalankan
bagi meneliti impak perbezaan suhu dan variasi diet pada parameter
hematologi dan serum biokimia pada juvenil Lutjanus malabaricus,
satu spesies ikan komersial penting di Malaysia. Ikan (16-18
cm) dibahagikan kepada lapan rawatan (4 suhu × 2 diet) dan
terdapat tiga replikat (5 ikan setiap replikat) bagi setiap rawatan.
Ikan terdedah kepada empat suhu yang berbeza (22, 26, 30 dan 34°C)
dan diberi makan pelet komersial dan diet udang semula jadi. Selepas
30 hari tempoh uji kaji, sampel darah diambil dan dipindahkan ke
dalam dua tiub yang berbeza daripada setiap sampel: satu yang mengandungi
EDTA
sebagai agen antikoagulan dan yang lain tanpa EDTA untuk
pengumpulan serum. Semua parameter hematologi dan biokimia dalam
semua rawatan berada dalam julat normal. Perubahan suhu dan diet
menyebabkan perubahan ketara (p <0.05) dalam jumlah panjang
(TL),
berat badan (BW), sel darah merah (RBC),
hemoglobin (HB), hematokrit (HC), trombosit (TC)
dan sel darah putih (WBC) tetapi perubahan jumlah min korpuskul
(MCV), min sel hemoglobin (MCH),
manakala nilai min kepekatan sel hemoglobin (MCHC)
tidak signifikan (p > 0.05). Nilai yang lebih tinggi dilihat
pada ikan yang diternak pada 26 dan 30°C daripada yang diternak
pada 22 dan 34°C (p <0.05). Jumlah protein (TP), albumin (ALB),
nisbah albumin/globin (nisbah A/G), alkali fosfatase (AP),
tahap aspartat aminotransferase (AST) dan alanine transaminase
(ALT)
juga meningkat dengan ketara pada 30°C dan 26°C berbanding
dengan 34°C dan 22°C. Tahap Globulin (GLB) menunjukkan keputusan yang bertentangan.
Dalam semua kes, prestasi juvenil lebih tinggi apabila diternak
menggunakan diet udang berbanding diet pelet. Parameter fisiologi
yang digunakan dalam kajian ini telah dicadangkan sebagai pemantau
kesihatan yang mudah dalam penternakan ikan.
Kata kunci: Diet; hematologi; ikan; serum biokimia; suhu
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*Pengarang
untuk surat-menyurat; email: skdas_maa@yahoo.com
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