Sains Malaysiana 49(4)(2020): 721-728 http://dx.doi.org/10.17576/jsm-2020-4904-01
Perbandingan Ciri Anatomi
Daun Tetrastigma rafflesiae (Miq.) Planchon
dan Tetrastigma pedunculare (Wall. ex Laws.) Planch. di Semenanjung Malaysia
(Comparative
Leaf Anatomy of Tetrastigma rafflesiae (Miq.) Planchon and Tetrastigma pedunculare (Wall. ex
Laws.) Planch. in Peninsular Malaysia)
SYAMSURINA
ARSHAD*, MOHD AFIQ AIZAT JUHARI, NORAINI TALIP, NOR AZILAH ABDUL WAHAB, SYUHADA
FADZILAH & JUMAAT ADAM
Pusat Pengajian
Sains Sekitaran dan Sumber Alam, Fakulti Sains dan Teknologi, Universiti
Kebangsaan Malaysia, 43650 UKM Bangi,
Selangor Darul Ehsan, Malaysia
Diserahkan: 18
September 2018/Diterima: 18 Disember 2019
ABSTRAK
Kajian
ini bertujuan untuk melakukan kajian terhadap ciri anatomi daun Tetrastigma
rafflesiae (Miq.) Planchon dan Tetrastigma
pedunculare (Wall. ex Laws.)
Planch. yang merupakan perumah kepada bunga pakma dan pakma
tikus di Semenanjung Malaysia. Sampel daun diperoleh daripada habitat asal iaitu Gunung Bubu, Gerik,
Perak. Teknik piawai dalam anatomi tumbuhan digunakan dan imbasan adalah
menggunakan mikroskop cahaya dan mikroskrop imbasan elektron (SEM) dengan
pemerhatian dilakukan meliputi bahagian petiol, tulang daun, lamina, epidermis
dan peruratan daun. Hasil menunjukkan kehadiran trikom dan sel arenkima hanya
terdapat pada T. pedunculare sahaja. Bilangan berkas vaskular medula pada T. rafflesiae adalah
tunggal, manakala T. pedunculare adalah tiga. Oleh itu, ketiga-tiga
ciri ini dapat membezakan dengan jelas kedua-dua spesies.
Kata kunci:
Anatomi daun; perumah; Tetrastigma pedunculare; Tetrastigma rafflesiae
ABSTRACT
This study
aimed to study the leaf anatomy of Tetrastigma rafflesiae (Miq.) Planchon and Tetrastigma
pedunculare (Wall. ex Laws.) Planch., the host of Rafflesia and Rhizanthes in
Peninsular Malaysia. Leaf samples
were collected in their natural habitat at Gunung Bubu, Gerik, Perak. Standard
techniques in plant anatomy were employed, including analyses under light and
scanning electron microscopy (SEM) with observation on the petioles,
midrib, laminae, leaf epidermis and also on the leaf venation. The results show
the
presence of trichome and arenchyma cell only at T. pedunculare. The number of medullary
vascular bundle of T. rafflesiae is only single but T. pedunculare are three. Therefore, the three characteristic can be used to differentiate
these two species.
Keywords: Host plants; leaf anatomy; Tetrastigma pedunculare; Tetrastigma rafflesiae
RUJUKAN
Akhriadi, P., Kiswanto,
H.A., Taufiq, A., Alfajri, D. & Kardiman, R. 2010. Assessment of
conservation status of Rafflesia in West Sumatra, Indonesia. Laporan
akhir kepada dana kecil Rufford (untuk pemuliharaan semula jadi). Pasukan pemantau Rafflesia (RMT) Padang, Indonesia.
Al-Edany, T.Y., Sahar,
A. & Al-Saadi, A.M. 2011. Taxonomic significance of anatomical characters
in some species of the family Myrtaceae. American Journal of Plant Sciences 3: 572-581.
Amalia, R., Noraini, T.,
Jalifah, L., Ruzi, A.R. & Idris, S. 2013. Morphology of trichomes in Pogostemon
cablin Benth. (Lamiaceae). Australia
Journal of Crop Science 7(6): 744-749.
Angyalossy, V., Angeles,
G., Pacea, M.R., Lima, A.C., Dias-Leme, C.L., Lohmann, L.G. & Madero-Vega,
C. 2012. An overview of the anatomy, development and evolution of the vascular
system of lianas. Plant Ecology and Diversity 5(2): 167-182.
Banziger, H. 1995.
Ecological, morphological and taxonomic studies on Thailand’s fifth species of
Rafflesiaceae: Rhizanthes zippelli (Blume) Spach. Natural History
Bulletin of the Siam Society 43: 337-365.
Baucher, M., El-Jaziri,
M. & Vandeputte, O. 2007. From primary to secondary growth: Origin and
development of the vascular system. Journal
of Experimental Botany 58(13): 3485-3501.
Beck, C.B. 2010. An Introduction to Plant Structure and
Development Plant Anatomy for the Twenty-First Century. Edisi ke-2.
Edinburgh: Cambridge University Press.
Chnar, N. 2014. Leaf
anatomy and palynological differences among selected cultivars of Vitis
vinifera and Parthenocissus quinquefolia (Vitaceae). The International Weekly Journal for Species 9(21): 6-12.
Chnar, N., Khatijah, H.
& Haja, M. 2013. Comparative leaf anatomy of selected species in Vitaceae
and Leeaceae. American Journal of Applied Sciences 10(4): 414-417.
Chnar, N., Khatijah, H.
& Haja, M. 2011. Comparative study on the anatomy and palynology of the
three variety of Vitis vinifera varity (family Vitaceae). African
Journal of Biotechnology 10(74): 16866-16874.
Cutler, D.F., Botha, T.
& Stevenson, D.W. 2007. Plant Anatomy: An Applied Approach. New York: Blackwell Publishing
Ltd.
Conklin, P.A., Strable,
J., Li, S. & Scanlon, M.J. 2019. On the mechanisms of development in
monocot and eudicot leaves. New
Phytologist 221: 706-724.
Diway, B.M. & Chai, P.P.K. 2004. Development
of Lanjak Entimau Wildlife Sanctuary as a totally protected area. A study on
the vegetation of Batang Ai National Park, Sarawak Malaysia. International Tropical
Timber Organization (ITTO), Forestry Department Sarawak Malaysia.
Febriyanti, E., Suwirmen
& Idris, M. 2013. Induksi perakaran tunas Tetrastigma rafflesiae Miq.
pada media Murashige-skoog dengan penambahan beberapa konsentrasi
indole-3-butyric acid (IBA) secara in
vitro. Jurnal Biologi Universitas Andalas 2(2): 161-167.
Glover, B.J. 2000.
Differentiation in plant epidermal cells. Journal
of Experimental Botany 51(344): 497-205.
Jackson, M.B. &
Amstrong, W. 1999. Formation of aerenchyma and the processes of plant
ventilation in relation to soil flooding and submergence. Plant Biology 1(3): 274-287.
Jamili, N. 2004. Rafflesia
Bunga Terbesar di Dunia. Kota Kinabalu: Natural History Publication.
Jamili, N. 2001. Rafflesia
of the World. Kota Kinabalu: Penerbit Sabah Parks.
Johansen, D.A. 1940. Plant
Microtechnique. New York: McGraw-Hill Book Company Inc.
Jumaat, H.A., Mohd Afiq
Aizat, J., Rahmah, M., Nor Azilah, A.W., Syamsurina, A., Mohd Paiz, K., Mohd
Firdaus, M.R. & Kiew-Lian, W. 2016. Rafflesia tuanku-halimii (Rafflesiaceae),
a new species from Peninsular Malaysia. Sains Malaysiana 45(11):
1589-1595.
Jumaat, H.A., Rahmah,
M., Mohd Afiq Aizat, J., Nik Nadira Farhana, N.A. & Kiew-Lian, W. 2013. Rafflesia
sharifah-hapsahiae (Rafflesiaceae), a new species from Peninsular Malaysia. Turkish Journal of Botany 37: 1038-1044.
Kacprzyk, J., Daly, C.T.
& McCabe, P.F. 2011. The botanical dance of death: Programmed cell death in
plants. Advance in Botanical Research
Journal 60: 169-261.
Kirkendall, L.R., Biedermann, P.H.W. & Jordal, B.H.
2015. Evolution and diversity of bark and ambrosia beetles. Chapter 3. Dlm. Bark Beetles: Biology and Ecology of Native
and Invesive Species, disunting oleh Vega, F.E. & Hofstetter, R.W.
London: Academic Press. hlm. 85-156.
Kochaiphat, P.,
Trias-Blasi, A. & Pornpongrungrueng, P. 2016. Two new species of
Tetrastigma (Miq.) Planch. (Vitaceae) from Thailand. European Journal of Taxonomy 201: 1-12.
Kochaiphat, P.,
Trias-Blasi, A. & Pornpongrungrueng, P. 2013. Comparative leaves anatomy of
some Cayratia Juss and Tetrastigma (Miq.) Planch. in Thailand. Proceedings of the 29th National Graduate
Research Conference, Mae Fah Luang University, Chiang Rai, Thailand. hlm.
883-891.
Klein, L.L., Caito, M.,
Chapnick, C., Kitchen, C., O’Hanlon, R., Chitwood, D.H. & Miller, A.J.
2017. Digital morphometrics of two North American grapevines (Vitis:Vitaceae) quantifies leaf variation between species, within
species, and among individuals. Frontiers
in Plant Science 8(373): 1-10.
Kumar, D. & Kellogg,
E.A. 2019. Getting closer: Vein density in C4 leaves. New Phytologist 221: 1260-1267.
Latiff, A. 2015. Studies
in Malesian Vitaceae XV. Revision of Tetrastigma (Miq.) Planch. sect. Carinata Latiff. Malayan Nature Journal 67(3): 318-327.
Latiff, A. 1983. Studies in
Malesian Vitaceae VII. The genus Tetrastigma in the Malay Peninsula. Gardens
Bulletin of Singapore 36(2): 213-228.
Lucas, W.J., Groover,
A., Lichtenberger, R., Furuta, K., Yadav, S.R., Helariutta, Y., He, Q., Fukuda,
H., Kang, J., Brady, S.M., Patrick, J.W., Sperry, J., Yoshida, A., Mill,
A.F.L., Grusak, M.A. & Kachroo, P. 2013. The plant vascular system:
Evolution, development and functions. Journal
of Integrative Plant Biology 55(4): 294-388.
Metcafe, C.R. &
Chalk, L. 1957. Anatomy of the Dicotyledons. Oxford: Clarendon Press.
Morais,
T.M.D.S., Rabelo, G.R.,
Alexandrino, C.R., Neto, S.J.D.S. & Cunha, M.D. 2011. Comparative leaf
anatomy and micromorphology of Psychotria species (Rubiaceae) from the
Atlantic Rainforest. Acta Botanica Brasilica 25(1): 178-190.
Muhammad, A.Z., Nor,
S.O., Zaini, Y., Mohd Lias, K. & Jamil, T. 2014. Identification of alkaloid
compound and antioxidant activity of Rafflesia cantleyi and its host, Tetrastigma
tuberculatum. The Open Conference Proceedings Journal 5: 18-20.
Nasihah, M., Zulhazman,
H., Siti Munirah, M.Y., Wan Norqayyum Nadia, W.A. & Latiff, A. 2016. Tetrastigma
hookeri (Laws.) Planch. (Vitaceae), a host plant for Rafflesia kerrii Meijer
in Peninsular Malaysia. Malayan Nature Journal 68(1&2): 33-39.
Noraini, T. &
Cutler, D.F. 2009. Leaf anatomical and micromorphological characters of some
Malaysian Parashorea (Dipterocarpaceae). Journal of Tropical Forest
Science 21(2): 156-167.
Nur
Aishah, A. 2016. Comparative leaf anatomy
of Tetrastigma species (Vitaceae) from Gunung Mulu National Park,
Sarawak. Tesis Sarjana Muda. Universiti Malaysia Sarawak (tidak diterbitkan).
Olivera, A.B., Mendonça,
M.S., Azevedo, A.A. & Meira, R.M.S.A. 2012. Anatomy and histochemistry of
the vegetative organs of Cissus verticillata - A native medicinal plant
of the Brazilian Amazon. Brazilian Journal of Pharmacognosy 22(6):
1201-1211.
Saadu, R.O.,
Abdulrahaman, A.A. & Oladele, F.A. 2009. Stomatal complex types and
transpiration rates in some tropical tuber species. African
Journal of Plant Science 3(5): 107-112.
Sack, L., Scoffoni, C.,
John, G.P., Poorter, H., Mason, C.M., Mendez-Alonzo, R. & Donovan, L.A.
2013. How do leaf veins influence the worldwide leaf economic spectrum? Review
and synthesis. Journal of Experimental
Botany 64(13): 4053-4080.
Sass, J.E. 1958. Botanical
Microtechnique. Edisi ke-3. Ames: Iowa State University Press.
Solihani, S.N., Noraini,
T., Ruzi, A.R & Chung, R.C.K. 2010. Nilai taksonomi ciri anatomi daun Coelostegia Benth. dan Ceiba pentandra Gaertn. (Bombacaceae). Sains Malaysiana 39(3): 371-376.
Takahashi, H., Yamauchi,
T., Colmer, T.D. & Nakazono, M. 2014. Aerenchyma formation in plants. In Low Oxygen Stress in Horticulture Practise,
edited by van Dongen, J.T. & Licausi, F. Springer: Vienna. hlm. 247-265.
Tan, A.L. 2006. Kajian Rafflesia Kuala Melantai Pahang. Tesis Sarjana Muda, Universiti Kebangsaan Malaysia (tidak diterbitkan).
Veldkamp, J.F. 2007. The
correct name for the Tetrastigma (Vitaceae) host of Rafflesia (Rafflesiaceae)
in Malesia and a (not so) new species. Reinwardtia 12(4): 261- 265.
Wan Nuur Fatihah, W.Z.,
Aida Shafreena, A.P., Geri, C., Ramlah, Z. & Latiff, A. 2016. Tetrastigma
diepenhorstii (Miq.) Latiff (Vitaceae), a new host of Rafflesia
tuan-mudae Becc. (Rafflesiaceae) in Borneo. Journal of Botany 2016:
3952323.
Wen, J. 2007. Vitaceae.
Dlm. The Families and Genera of Vascular
Plants Volume IX Flowering Plants Eudicots, disunting oleh Kubitzki, K.
Berlin: Springer-Verlag. hlm. 467-479.
Wen, J., Lu, L. &
Boggan, J.K. 2013. Diversity and evolution of Vitaceae in the Philippines. Philippine
Journal of Science Special Issue 142: 223-244.
Ye, Z.H. 2002. Vascular
tissue differentiation and pattern formation in plants. Annual Review of Plant Biology 53: 183-202.
*Pengarang
untuk surat-menyurat; email: p70780@siswa.ukm.edu.my
|