Sains Malaysiana 49(4)(2020): 721-728

http://dx.doi.org/10.17576/jsm-2020-4904-01

 

Perbandingan Ciri Anatomi Daun Tetrastigma rafflesiae (Miq.) Planchon dan Tetrastigma pedunculare (Wall. ex Laws.) Planch. di Semenanjung Malaysia

(Comparative Leaf Anatomy of Tetrastigma rafflesiae (Miq.) Planchon and Tetrastigma pedunculare (Wall. ex Laws.) Planch. in Peninsular Malaysia)

 

SYAMSURINA ARSHAD*, MOHD AFIQ AIZAT JUHARI, NORAINI TALIP, NOR AZILAH ABDUL WAHAB, SYUHADA FADZILAH & JUMAAT ADAM

 

Pusat Pengajian Sains Sekitaran dan Sumber Alam, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43650 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

Diserahkan: 18 September 2018/Diterima: 18 Disember 2019

 

ABSTRAK

Kajian ini bertujuan untuk melakukan kajian terhadap ciri anatomi daun Tetrastigma rafflesiae (Miq.) Planchon dan Tetrastigma pedunculare (Wall. ex Laws.) Planch. yang merupakan perumah kepada bunga pakma dan pakma tikus di Semenanjung Malaysia. Sampel daun diperoleh daripada habitat asal iaitu Gunung Bubu, Gerik, Perak. Teknik piawai dalam anatomi tumbuhan digunakan dan imbasan adalah menggunakan mikroskop cahaya dan mikroskrop imbasan elektron (SEM) dengan pemerhatian dilakukan meliputi bahagian petiol, tulang daun, lamina, epidermis dan peruratan daun. Hasil menunjukkan kehadiran trikom dan sel arenkima hanya terdapat pada T. pedunculare sahaja. Bilangan berkas vaskular medula pada T. rafflesiae adalah tunggal, manakala T. pedunculare adalah tiga. Oleh itu, ketiga-tiga ciri ini dapat membezakan dengan jelas kedua-dua spesies.

 

Kata kunci: Anatomi daun; perumah; Tetrastigma pedunculare; Tetrastigma rafflesiae

 

ABSTRACT

This study aimed to study the leaf anatomy of Tetrastigma rafflesiae (Miq.) Planchon and Tetrastigma pedunculare (Wall. ex Laws.) Planch., the host of Rafflesia and Rhizanthes in Peninsular Malaysia. Leaf samples were collected in their natural habitat at Gunung Bubu, Gerik, Perak. Standard techniques in plant anatomy were employed, including analyses under light and scanning electron microscopy (SEM) with observation on the petioles, midrib, laminae, leaf epidermis and also on the leaf venation. The results show the presence of trichome and arenchyma cell only at T. pedunculare. The number of medullary vascular bundle of T. rafflesiae is only single but T. pedunculare are three. Therefore, the three characteristic can be used to differentiate these two species.

 

Keywords: Host plants; leaf anatomy; Tetrastigma pedunculare; Tetrastigma rafflesiae

 

RUJUKAN

Akhriadi, P., Kiswanto, H.A., Taufiq, A., Alfajri, D. & Kardiman, R. 2010. Assessment of conservation status of Rafflesia in West Sumatra, Indonesia. Laporan akhir kepada dana kecil Rufford (untuk pemuliharaan semula jadi). Pasukan pemantau Rafflesia (RMT) Padang, Indonesia.

Al-Edany, T.Y., Sahar, A. & Al-Saadi, A.M. 2011. Taxonomic significance of anatomical characters in some species of the family Myrtaceae. American Journal of Plant Sciences 3: 572-581.

Amalia, R., Noraini, T., Jalifah, L., Ruzi, A.R. & Idris, S. 2013. Morphology of trichomes in Pogostemon cablin Benth. (Lamiaceae). Australia Journal of Crop Science 7(6): 744-749.

Angyalossy, V., Angeles, G., Pacea, M.R., Lima, A.C., Dias-Leme, C.L., Lohmann, L.G. & Madero-Vega, C. 2012. An overview of the anatomy, development and evolution of the vascular system of lianas. Plant Ecology and Diversity 5(2): 167-182.

Banziger, H. 1995. Ecological, morphological and taxonomic studies on Thailand’s fifth species of Rafflesiaceae: Rhizanthes zippelli (Blume) Spach. Natural History Bulletin of the Siam Society 43: 337-365.

Baucher, M., El-Jaziri, M. & Vandeputte, O. 2007. From primary to secondary growth: Origin and development of the vascular system. Journal of Experimental Botany 58(13): 3485-3501.

Beck, C.B. 2010. An Introduction to Plant Structure and Development Plant Anatomy for the Twenty-First Century. Edisi ke-2. Edinburgh: Cambridge University Press. 

Chnar, N. 2014. Leaf anatomy and palynological differences among selected cultivars of Vitis vinifera and Parthenocissus quinquefolia (Vitaceae). The International Weekly Journal for Species 9(21): 6-12. 

Chnar, N., Khatijah, H. & Haja, M. 2013. Comparative leaf anatomy of selected species in Vitaceae and Leeaceae. American Journal of Applied Sciences 10(4): 414-417.

Chnar, N., Khatijah, H. & Haja, M. 2011. Comparative study on the anatomy and palynology of the three variety of Vitis vinifera varity (family Vitaceae). African Journal of Biotechnology 10(74): 16866-16874.

Cutler, D.F., Botha, T. & Stevenson, D.W. 2007. Plant Anatomy: An Applied Approach. New York: Blackwell Publishing Ltd.

Conklin, P.A., Strable, J., Li, S. & Scanlon, M.J. 2019. On the mechanisms of development in monocot and eudicot leaves. New Phytologist 221: 706-724.

Diway, B.M. & Chai, P.P.K. 2004. Development of Lanjak Entimau Wildlife Sanctuary as a totally protected area. A study on the vegetation of Batang Ai National Park, Sarawak Malaysia. International Tropical Timber Organization (ITTO), Forestry Department Sarawak Malaysia.

Febriyanti, E., Suwirmen & Idris, M. 2013. Induksi perakaran tunas Tetrastigma rafflesiae Miq. pada media Murashige-skoog dengan penambahan beberapa konsentrasi indole-3-butyric acid (IBA) secara in vitro. Jurnal Biologi Universitas Andalas 2(2): 161-167.

Glover, B.J. 2000. Differentiation in plant epidermal cells. Journal of Experimental Botany 51(344): 497-205.

Jackson, M.B. & Amstrong, W. 1999. Formation of aerenchyma and the processes of plant ventilation in relation to soil flooding and submergence. Plant Biology 1(3): 274-287.

Jamili, N. 2004. Rafflesia Bunga Terbesar di Dunia. Kota Kinabalu: Natural History Publication.

Jamili, N. 2001. Rafflesia of the World. Kota Kinabalu: Penerbit Sabah Parks.

Johansen, D.A. 1940. Plant Microtechnique. New York: McGraw-Hill Book Company Inc.

Jumaat, H.A., Mohd Afiq Aizat, J., Rahmah, M., Nor Azilah, A.W., Syamsurina, A., Mohd Paiz, K., Mohd Firdaus, M.R. & Kiew-Lian, W. 2016. Rafflesia tuanku-halimii (Rafflesiaceae), a new species from Peninsular Malaysia. Sains Malaysiana 45(11): 1589-1595.

Jumaat, H.A., Rahmah, M., Mohd Afiq Aizat, J., Nik Nadira Farhana, N.A. & Kiew-Lian, W. 2013. Rafflesia sharifah-hapsahiae (Rafflesiaceae), a new species from Peninsular Malaysia. Turkish Journal of Botany 37: 1038-1044.

Kacprzyk, J., Daly, C.T. & McCabe, P.F. 2011. The botanical dance of death: Programmed cell death in plants. Advance in Botanical Research Journal 60: 169-261.

Kirkendall, L.R., Biedermann, P.H.W. & Jordal, B.H. 2015. Evolution and diversity of bark and ambrosia beetles. Chapter 3. Dlm. Bark Beetles: Biology and Ecology of Native and Invesive Species, disunting oleh Vega, F.E. & Hofstetter, R.W. London: Academic Press. hlm. 85-156.

Kochaiphat, P., Trias-Blasi, A. & Pornpongrungrueng, P. 2016. Two new species of Tetrastigma (Miq.) Planch. (Vitaceae) from Thailand. European Journal of Taxonomy 201: 1-12.

Kochaiphat, P., Trias-Blasi, A. & Pornpongrungrueng, P. 2013. Comparative leaves anatomy of some Cayratia Juss and Tetrastigma (Miq.) Planch. in Thailand. Proceedings of the 29th National Graduate Research Conference, Mae Fah Luang University, Chiang Rai, Thailand. hlm. 883-891.

Klein, L.L., Caito, M., Chapnick, C., Kitchen, C., O’Hanlon, R., Chitwood, D.H. & Miller, A.J. 2017. Digital morphometrics of two North American grapevines (Vitis:Vitaceae) quantifies leaf variation between species, within species, and among individuals. Frontiers in Plant Science 8(373): 1-10.

Kumar, D. & Kellogg, E.A. 2019. Getting closer: Vein density in C4 leaves. New Phytologist 221: 1260-1267.

Latiff, A. 2015. Studies in Malesian Vitaceae XV. Revision of Tetrastigma (Miq.) Planch. sect. Carinata Latiff. Malayan Nature Journal 67(3): 318-327.

Latiff, A. 1983. Studies in Malesian Vitaceae VII. The genus Tetrastigma in the Malay Peninsula. Gardens Bulletin of Singapore 36(2): 213-228.

Lucas, W.J., Groover, A., Lichtenberger, R., Furuta, K., Yadav, S.R., Helariutta, Y., He, Q., Fukuda, H., Kang, J., Brady, S.M., Patrick, J.W., Sperry, J., Yoshida, A., Mill, A.F.L., Grusak, M.A. & Kachroo, P. 2013. The plant vascular system: Evolution, development and functions. Journal of Integrative Plant Biology 55(4): 294-388.

Metcafe, C.R. & Chalk, L. 1957. Anatomy of the Dicotyledons. Oxford: Clarendon Press.

Morais, T.M.D.S., Rabelo, G.R., Alexandrino, C.R., Neto, S.J.D.S. & Cunha, M.D. 2011. Comparative leaf anatomy and micromorphology of Psychotria species (Rubiaceae) from the Atlantic Rainforest. Acta Botanica Brasilica 25(1): 178-190.

Muhammad, A.Z., Nor, S.O., Zaini, Y., Mohd Lias, K. & Jamil, T. 2014. Identification of alkaloid compound and antioxidant activity of Rafflesia cantleyi and its host, Tetrastigma tuberculatum. The Open Conference Proceedings Journal 5: 18-20.

Nasihah, M., Zulhazman, H., Siti Munirah, M.Y., Wan Norqayyum Nadia, W.A. & Latiff, A. 2016. Tetrastigma hookeri (Laws.) Planch. (Vitaceae), a host plant for Rafflesia kerrii Meijer in Peninsular Malaysia. Malayan Nature Journal 68(1&2): 33-39.

Noraini, T. & Cutler, D.F. 2009. Leaf anatomical and micromorphological characters of some Malaysian Parashorea (Dipterocarpaceae). Journal of Tropical Forest Science 21(2): 156-167.

Nur Aishah, A. 2016. Comparative leaf anatomy of Tetrastigma species (Vitaceae) from Gunung Mulu National Park, Sarawak. Tesis Sarjana Muda. Universiti Malaysia Sarawak (tidak diterbitkan).

Olivera, A.B., Mendonça, M.S., Azevedo, A.A. & Meira, R.M.S.A. 2012. Anatomy and histochemistry of the vegetative organs of Cissus verticillata - A native medicinal plant of the Brazilian Amazon. Brazilian Journal of Pharmacognosy 22(6): 1201-1211.

Saadu, R.O., Abdulrahaman, A.A. & Oladele, F.A. 2009. Stomatal complex types and transpiration rates in some tropical tuber species.  African Journal of Plant Science 3(5): 107-112.

Sack, L., Scoffoni, C., John, G.P., Poorter, H., Mason, C.M., Mendez-Alonzo, R. & Donovan, L.A. 2013. How do leaf veins influence the worldwide leaf economic spectrum? Review and synthesis. Journal of Experimental Botany 64(13): 4053-4080.

Sass, J.E. 1958. Botanical Microtechnique. Edisi ke-3. Ames: Iowa State University Press.

Solihani, S.N., Noraini, T., Ruzi, A.R & Chung, R.C.K. 2010. Nilai taksonomi ciri anatomi daun Coelostegia Benth. dan Ceiba pentandra Gaertn. (Bombacaceae). Sains Malaysiana 39(3): 371-376.

Takahashi, H., Yamauchi, T., Colmer, T.D. & Nakazono, M. 2014. Aerenchyma formation in plants. In Low Oxygen Stress in Horticulture Practise, edited by van Dongen, J.T. & Licausi, F. Springer: Vienna. hlm. 247-265.

Tan, A.L. 2006. Kajian Rafflesia Kuala Melantai Pahang. Tesis Sarjana Muda, Universiti Kebangsaan Malaysia (tidak diterbitkan).

Veldkamp, J.F. 2007. The correct name for the Tetrastigma (Vitaceae) host of Rafflesia (Rafflesiaceae) in Malesia and a (not so) new species. Reinwardtia 12(4): 261- 265.

Wan Nuur Fatihah, W.Z., Aida Shafreena, A.P., Geri, C., Ramlah, Z. & Latiff, A. 2016. Tetrastigma diepenhorstii (Miq.) Latiff (Vitaceae), a new host of Rafflesia tuan-mudae Becc. (Rafflesiaceae) in Borneo. Journal of Botany 2016: 3952323.

Wen, J. 2007. Vitaceae. Dlm. The Families and Genera of Vascular Plants Volume IX Flowering Plants Eudicots, disunting oleh Kubitzki, K. Berlin: Springer-Verlag. hlm. 467-479.

Wen, J., Lu, L. & Boggan, J.K. 2013. Diversity and evolution of Vitaceae in the Philippines. Philippine Journal of Science Special Issue 142: 223-244.

Ye, Z.H. 2002. Vascular tissue differentiation and pattern formation in plants. Annual Review of Plant Biology 53: 183-202.

 

*Pengarang untuk surat-menyurat; email: p70780@siswa.ukm.edu.my