Sains Malaysiana 49(6)(2020): 1333-1344

http://dx.doi.org/10.17576/jsm-2020-4906-11

Fenitrothion Impaired Sexual Behaviour and Reproductive Performance in Male Sprague-Dawley Rats

(Fenitrotion Menjejaskan Tingkah Laku Seksual dan Prestasi Reproduktif Tikus Jantan Sprague-Dawley)

NUR AFIZAH YUSOFF¹, MAHANEEM MOHAMED², SITI BALKIS BUDIN¹ & IZATUS SHIMA TAIB¹*

¹Biomedical Science Programme, Centre of Diagnostic, Therapeutic and Investigations, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Federal Territory, Malaysia

²Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan Darul Naim, Malaysia

Diserahkan: 14 Ogos 2019/ Diterima: 31 Januari 2020

ABSTRACT

Fenitrothion (FNT) induces oxidative damage in various organs including male reproductive organ such as testis and sperm; however, its effects on the male sexual behaviours and reproductive performance remain unclear. Therefore, this study aimed to evaluate the effects of FNT administration on male sexual behaviour and reproductive performance. Fertile male Sprague-Dawley rats were randomly divided into three groups (n=8/group): Control - receiving corn oil (1 mL kg^-1); FNT-10 and FNT-20 receiving 10 and 20 mg kg^-1 of FNT, respectively. FNT was administered via oral force feeding for 28 consecutive days prior to mating with untreated female rats. After mating, the rats were sacrificed to obtain plasma and sperm for further evaluation. Results showed that FNT-20 alone decreased AChE activity (P<0.05) compared to the control and FNT-10. Both doses of FNT also decreased sperm quality compared to control (P<0.05). A significant difference was found (P<0.05) for the presence of intromission and ejaculation among all groups. The mount and intromission latencies as well as post-ejaculation interval were increased (P<0.05) in FNT groups compared to the control group. The total mount and mount frequency were decreased (P<0.05) in FNT-10 and FNT-20 compared to the controls group. For reproductive performance, there was a significant difference (P<0.05) for the mating and pregnancy indexes among all groups. Furthermore, both doses of FNT reduced the number of dams, delivered pups and male pups compared to the control (P<0.05). In conclusion, FNT impaired sexual behaviour and reproductive performance of male rats.

Keywords: Infertility; organophosphate; parturition; sexual behaviour; sperm damage

ABSTRAK

Fenitrotion (FNT) mengaruh kerosakan oksidatif dalam pelbagai organ termasuklah organ reproduktif lelaki seperti testis dan sperma; walau bagaimanapun, kesannya terhadap tingkah laku seksual dan prestasi reproduktif masih belum diketahui. Oleh itu, kajian ini bertujuan untuk menilai kesan pemberian FNT terhadap tingkah laku seksual dan prestasi reproduktif. Tikus jantan Sprague-Dawley subur dibahagikan secara rawak kepada tiga kumpulan (n=8/kumpulan): Kawalan - menerima minyak jagung (1 mL kg^-1); FNT-10 dan FNT-20 menerima 10 dan 20 mg kg^-1 FNT, setiap satunya. FNT diberikan secara oral paksa berturutan selama 28 hari sebelum pengawanan dilakukan dengan tikus betina tidak dirawat. Selepas pengawanan, tikus dikorbankan untuk mendapatkan plasma dan sperma bagi penilaian selanjutnya. Keputusan menunjukkan FNT-20 sahaja menurunkan aktiviti AChE (P<0.05) berbanding kumpulan kawalan dan FNT-10. Kedua-dua dos FNT juga menurunkan kualiti sperma berbanding kumpulan kawalan (P<0.05). Terdapat perbezaan signifikan (P<0.05) bagi kehadiran intromisi dan ejakulasi antara semua kumpulan. Latensi pemanjatan dan intromisi serta selang selepas ejakulasi turut meningkat (P<0.05) pada kumpulan FNT berbanding kumpulan kawalan. Sebaliknya, hanya FNT-20 yang menyebabkan pengurangan frekuensi intromisi dan peningkatan latensi ejakulasi berbanding kumpulan kawalan (P<0.05). Jumlah dan frekuensi pemanjatan adalah menurun (P<0.05) pada kumpulan FNT-10 dan FNT-20 berbanding kumpulan kawalan. Bagi prestasi reproduktif, terdapat perbezaan signifikan (P<0.05) pada indeks pengawanan dan kehamilan antara semua kumpulan. Tambahan lagi, kedua-dua dos FNT telah mengurangkan bilangan betina hamil, progeni yang dilahirkan dan progeni jantan berbanding kumpulan kawalan (P<0.05). Kesimpulannya, FNT menjejaskan tingkah laku seksual dan prestasi reproduktif tikus jantan.

Kata kunci: Kelahiran; ketaksuburan; kerosakan sperma; organofosfat; tingkah laku seksual

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*Pengarang untuk surat-menyurat; email: izatusshima@ukm.edu.my