Sains Malaysiana 51(10)(2022): 3195-3202

http://doi.org/10.17576/jsm-2022-5110-07

 

Monosaccharide Profile and Antioxidant Activities of Sulphated Polysaccharide Extracted from Indonesian Brown Seaweed (Sargassum sp.)

(Profil Monosakarida dan Aktiviti Antioksida Polisakarida Tersulfat yang Diekstrak daripada Rumpai Laut Perang Indonesia (Sargassum sp.))

 

SENG JOE LIM1,2,*, SHARIFAH HABIBAH SYED KHALAFU1, STEFAN BÖHMDORFER3, THOMAS ROSENAU3, MOHAMAD YUSOF MASKAT1,2, NOOR SOFFALINA SOFIAN SENG1,2, NOORUL SYUHADA MOHD RAZALI1,2 & WAN AIDA WAN MUSTAPHA1,2

 

1Department of Food Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

2Innovation Centre for Confectionery Technology (MANIS), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

3University of Natural Resources and Life Sciences, Vienna, Department of Chemistry, Institute for Chemistry of Renewable Resources, Muthgasse 18, A-1190 Vienna, Austria

 

Diserahkan: 5 Jun 2022/Diterima: 1 Ogos 2022

 

Abstract

This research was conducted to determine the monosaccharide profile and antioxidant activities of the sulphated polysaccharide fucoidan, extracted from Sargassum sp. harvested in Batam, Indonesia. Crude fucoidan (Fsar) was extracted using low pH (pH 2) acid method, obtaining a yield of 5.5% (w/w), and the chemical properties of Fsar were subsequently compared to commercial fucoidan (Fcomm), obtained from Japan. Sulphate contents were determined using spectrophotometric analysis, where Fsar (5.21±0.35%) and Fcomm (8.80±0.19%) differed significantly (p<0.05). Monosaccharide profiling was performed using methanolysis and gas chromatography-flame ionisation detector (GC-FID). Fsar contained fucose (138.34 µg/g), 4-O-methyl-glucuronic acid (105.39 µg/g), galactose (70.49 µg/g) and glucuronic acid (30.60 µg/g) while Fcomm contained fucose (520 µg/g) and glucuronic acid (62.93 µg/g) as its main composition. Antioxidant activities were analysed through 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion (SOA) and hydroxyl radical (·OH) scavenging activities. DPPH scavenging activity of Fsar (40.67±4.0%) was significantly higher (p<0.05) than Fcomm(10.11±1.0%), while SOA and ·OH scavenging activities showed no significant difference (p>0.05) between Fsar and Fcomm. Fsar showed significant differences (p<0.05) in chemical properties and DPPH scavenging activities compared to that of Fcomm. Fucoidan from Southeast Asia waters has the potential for application as functional ingredients.

 

Keywords: Brown seaweed; DPPH; fucoidan; monosaccharide profiling; sulphated polysaccharides

 

Abstrak

Penyelidikan ini dijalankan untuk mengenal pasti profil monosakarida dan aktiviti antioksida polisakarida tersulfat fukoidan yang diekstrak daripada Sargassum sp., dari Batam, Indonesia. Fukoidan kasar (Fsar) diekstrak dengan kaedah pengekstrakan asid pada pH rendah (pH 2) dan telah memperoleh hasil pengekstrakan sebanyak 5.5% (w/w). Seterusnya, ciri kimia Fsar telah dibandingkan dengan fukoidan komersial dari Jepun (Fcomm). Kandungan sulfat dikenal pasti menggunakan kaedah spektrofotometrik dengan Fsar (5.21±0.35%) dan Fcomm (8.80±0.19%) menunjukkan perbezaan yang signifikan (p<0.05). Pemprofilan monosakarida dijalankan menggunakan kaedah metanolisis dan kromatografi gas-pengesan nyala pengionan (GC-FID). Fsar didapati mengandungi fukosa (138.34 µg/g), asid 4-O-methil-glukuronik (105.39 µg/g), galaktosa (70.49 µg/g) dan asid glukuronik (30.60 µg/g); manakala Fcomm mengandungi fukosa (520 µg/g) dan asid glukuronik (62.93 µg/g) sebagai monosakarida utama. Aktiviti antioksidan dianalisis menggunakan kaedah pemerangkapan radikal bebas2,2-difenil-1-pikrilhidrazil (DPPH), anion superoksida (SOA) dan radikal hidroksil (·OH). Fsar (40.67±4.0%) menunjukkan aktiviti pemerangkapan radikal bebas DPPH yang lebih tinggi secara signifikan (p<0.05) berbanding Fcomm(10.11±1.0%), manakala aktiviti pemerangkapanSOA dan ·OH tidak menunjukkan perbezaan signifikan (p>0.05) antara Fsar dan Fcomm. Secara keseluruhannya, Fsar menunjukkan perbezaan yang signifikan (p<0.05) dalam ciri kimia dan aktiviti pemerangkapan radikal bebas DPPH berbanding Fcomm. Ini menunjukkan fukoidan dari perairan Asia Tenggara mempunyai potensi untuk dibangunkan sebagai ingredien kefungsian. 

 

Kata kunci: DPPH; fukoidan; pemprofilan monosakarida; polisakarida tersulfat; rumpai laut perang

 

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*Pengarang untuk surat-menyurat; email: joe@ukm.edu.my

 

 

 

 

   

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