Malaysian Journal of Analytical Sciences Vol 19 No 1 (2015): 138 – 143

 

 

 

ISOLATION OF BACTERIAL STRAIN FOR BIODEGRADATION OF FATS, OIL AND GREASE

 

(Pemencilan Strain Bakteria untuk Biodegradasi Lemak, Minyak dan Gris)

 

Ma’an F. Alkhatib*, Md. Zahangir Alam, Haytham F. M. Shabana

 

Bioenvironmental Engineering Research Center (BERC),

Biotechnology Engineering Department, Faculty of Engineering,

International Islamic University Malaysia (IIUM), 53100 Kuala Lumpur, Malaysia

 

*Corresponding author: maan@iium.edu.my

 

 

Abstract

Fat, oil and grease (FOG) deposition is one of the major problems that harm the environment and cause dissatisfaction for human. Uncontrolled and un-pre-treated FOG removal from the kitchen could lead to its accumulation in the piping system. Problems include the interference of fat with the aerobic microorganisms that are responsible in treating the wastewater by reducing oxygen transfer rates and for anaerobic microorganisms; their efficiency could also be reduced due to the reduction of the transport of soluble substrates to the bacterial biomass. Biodegradation could be one of the effective means to treat FOG. The main objective of this study is to isolate bacterial strains from the FOG waste and identify the strains that are capable in biodegrading FOG waste. FOG sample was collected from a sewer manhole. Enrichment technique was applied, followed by isolation of bacterial strains to determine which strain is able to degrade the FOG deposition. Some morphology for the bacterial strain was done to determine its characteristics.

 

Keywords:  fat, oil, grease, bacterial strain

 

Abstrak

Pemendapan lemak, minyak dan gris (FOG) adalah salah satu masalah utama yang membahayakan alam sekitar dan menyebabkan kegusaran dalam masyarakat. Penyingkiran FOG yang tidak terkawal dan tidak terawat daripada dapur boleh membawa kepada pengumpulan dalam sistem saluran paip. Masalah termasuk gangguan lemak dengan mikroorganisma aerobik yang bertanggungjawab dalam merawat air dengan mengurangkan kadar pemindahan oksigen dan anaerobik mikroorganisma; kecekapan mereka dapat juga dikurangkan akibat daripada pengurangan pengangkutan larut substrat untuk biojisim bakteria. Biodegradasi boleh menjadi salah satu cara yang berkesan untuk merawat FOG. Objektif utama kajian ini adalah untuk mengasingkan strain bakteria daripada bahan buangan FOG dan mengenal pasti strain yang mampu melakukan biodegradasi sisa FOG. FOG sampel diambil dari tempat sisa pembentungan. Teknik pengkayaan adalah digunakan, diikuti dengan pengasingan strain bakteria untuk menentukan strain yang dapat mengurai pemendapan FOG. Morfologi beberapa untuk strain bakteria telah dilakukan untuk menentukan ciri-ciri.

 

Kata kunci: lemak, minyak, gris, strain bakteria

 

References

1.       Jameel, A. T., Alade, A., Muyubi, S., Abdul Karim, M. I., and Alam, Z. (2011). Removal of Oil and Grease as Emerging Pollutants of Concern (EPC) in Wastewater Stream. IIUM Engineering Journal.

2.       Mendiola, S., Achutegui, J.J., Sanchez, F.J., and San, M.J. (1998) Polluting potential wastewater from fish-meal and oil industries. GrasaAceites , 49, 30–33

3.       Friedler, E. (2004). Quality of individual domestic greywater streams and its implication for on-site treatment and reuse possibilities. Environmental Technolology, 25, 997–1008.

4.       Lalman, J.A., and Bagley, D.M. (2000). Anaerobic degradation and inhibitory effects of linoleic acid. Water Res., 34(17), 4220–4228.

5.       Tano-Debrah, K., Fukuyama, S., Otonari, N., Taniguchi, F., and Ogura, M. (1999). An inoculum for the aerobic treatment of wastewaters with high concentrations of fats and oils. Bioresour. Technol. 69, 133–139.

6.       Chipasa, K. B. and Medrzycka, K. (2006). Behaviour of lipids in biological wastewater treatment processes. Journal of Industrial Microbiology Biotechnology, 33, 635‐645.

7.       Baig, N., and Grenning, E. M. (1976). The use of bacteria to reduce clogging of sewer lines by grease in municipal sewage. In: Tourbier J, Pierson RW (eds) Biological Control of Water Pollution, University of Pennsylvania Press, 245–253

8.       Chao, A.C., and Yang, W. (1981). Biological treatment of wool scouring wastewater. Journal of the Water Pollution Control Federation,  53(3), 311–317

9.       Rinzema, A., Boone, M., Van Knippenberg, K., and Lettinga, G. (1994) Bactericidal effect of long chain fatty acids in anaerobic digestion. Water Environmental Research, 66, 40–49

10.    Aluyor, E. O., Ori-jesu, M., and Obahiagbon, K. O. (2009). Biodegradation of vegetable oils: A review. Scientific Research and Essay 4, 543-548.

11.    Erguderet T H, Guven E, and Demirer G N (2000). Anaerobic treatment of olive oil waste water in batch reactor, Process Biochemistry 36(3) :234-248

12.    Kissi M, Mountadar M, Assobhei O, Gargiulo E, Palmieri G, and Giardina P, (2001). Roles of two white-rot basidiomycete fungi in decolorization and detoxification of olive mill wastewater. Applied Microbiology and Biotechnology, 57(1-2): 221–226

13.    Ettayebi, K., Errachidi, F., Jamai, L., Tahri-Jouti, A. M., Sendide, K., and Ettayebi, M. (2003) Biodegradation of polyphenols with immobilized Candida tropicalisunder metabolic induction FEMS Microbiology Letters, 223(2), 215–219.

14.    Ammar E, Nasri M, and Medhioub K, (2005). Isolation of phenol degrading Enterobacteria from the wastewater of olive oil extraction process. World Journal of Microbiology and Biotechnology, 21(3): 253–259

15.    Maier R. (2003). Biosurfactants: Evolution and Diversity in Bacteria. Advances in Applied Microbiology, 52: 101-121.

16.    Gottschalk, G. (1986). Bacterial Metabolism, 2nd Edition. Springer-Verlag. New York.

17.    Zubay, G. (1998). Biochemistry, 4th Edition. Wm. C. Brown Publishers, Dubuque, IA.

18.    Kleyn and Bicknell (2005). Microbiology Experiments: A Health Science Perspective McGraw-Hill Higher Education

19.    Čipinytė, V.,Grigiškis, S., Šapokaitė,D. and Baškys, E. (2011). Production of bio surfactance by Arthrobactersp. hydrocarbon degrading bacterium environment. Technology Resources, Proceedings of the 8th International Scientific and Practical Conference. Volume 1.

20.    Mudili, (2007) Jayababu Mudili, Introductionary Practical Microbiology, Alpha Science International Limited

21.    Thomsan J. N, 1999 microbiology lab manual Williams & Wilkins. Baltimore, MD

22.    Bergey, D. H., Krieg, N.R., and Holt, J.G. (1984). Bergey’s Manual of Systematic Bacteriology. Williams & Wilkins. Baltimore, MD

23.    Pepe, O., Blaiotta, G., Moschetti, G., Greco, T., and Villani, F. (2003). Rope-producing strains of Bacillus spp. from wheat bread and strategy for their control by lactic acid bacteria. Appl. Environ. Microbiol. 69 (4), 2321–2329.