Malaysian Journal of Analytical Sciences Vol 23 No 1 (2019): 14 - 22

A NEW ULTRA VIOLET-VISIBLE SPECTROPHOTOMETRIC METHOD FOR QUANTITATIVE DETERMINATION OF ACRYLAMIDE VIA HYDROLYSIS PROCESS

(Kaedah Spektrofotometrik Ultra Violet-Cahaya Nampak Baharu untuk Penentuan Akrilamida Secara Kuantitatif Melalui Proses Hidrolisis)

Yee-May Chong¹, Musa Ahmad²*, Lee Yook Heng¹

¹School of Chemical Sciences and Food Technology, Faculty Sciences & Technology,

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Malaysia

²Industrial Chemical Technology Programme, Faculty Sciences & Technology,

Universiti Sains Islam Malaysia, 71800 Nilai, Malaysia

*Corresponding author: andong@usim.edu.my

Received: 5 August 2018; Accepted: 8 December 2018

Abstract

This paper reported the results for quantitative determination of acrylamide via hydrolysis process using ultra violet-visible (UV-Vis) spectrophotometric method. This quantitative determination started with hydrolysing acrylamide in a strong basic condition to yield ammonia and acid salt. The optimum conditions of hydrolysis (concentration of the base used and time for hydrolysis) were also determined. From this study, the optimum conditions to hydrolyse acrylamide were achieved using 6.0 M of sodium hydroxide (NaOH) for 10 minutes. The hydrolysis process was characterised by monitoring the ammonia produced using Nessler’s reagent through the formation of yellow colouration in the presence of ammonia. After the optimisation of hydrolysis process, the characterisation of all parameters including concentration of Nessler’s reagent used, reproducibility, dynamic range, and interference ions were studied. Linear dynamic ranges from 0-10 ppm acrylamide with limit of detection (LOD) of 0.074 ppm were obtained when 3.0 mM Nessler’s reagent was used. The relative standard deviations (RSD) for reproducibility were 2.8-3.3%. No significant interference from cations such as Na⁺, K⁺, Ca²⁺ during quantitative analysis of acrylamide, but ions such as Fe³⁺ and NH₃ affected the analysis.

Keywords: acrylamide, hydrolysis, Nessler’s reagent

Abstrak

Kajian ini melaporkan keputusan untuk penentuan kuantitatif akrilamida melalui proses hidrolisis dengan kaedah spektofotometrik UV-Nampak (UV-Vis). Penentuan kuantitatif ini dimulai dengan hidrolisis akrilamida dalam keadaan bes kuat dan menghasilkan ammonia dan garam asid sebagai hasil. Keadaan optimum hidrolisis seperti kepekatan bes yang digunakan serta masa hidrolisis optimum telah ditentukan. Daripada kajian, keadaan hidrolisis akrilamida yang optimum ialah menggunakan natrium hidroksida (NaOH) 6.0 M dan masa tindak balas selama 10 minit. Proses hidrolisis telah dicirikan dengan memantau ammonia yang terhasil menggunakan reagen Nessler yang akan menghasilkan warna kuning dengan kehadiran ammonia. Setelah mengoptimumkan proses hidrolisis, pencirian semua parameter termasuk kepekatan reagen Nessler yang digunakan, kebolehulangan, julat dinamik, dan ganguan ion telah dikaji. Julat dinamik linear daripada 0-10 ppm akrilamida dengan had pengesanan (LOD) 0.074 ppm telah diperolehi apabila reagen Nessler 3.0 mM digunakan. Sisihan piawai relatif (RSD) untuk kebolehulangan telah diperolehi pada julat 2.8-3.3%. Kation seperti Na⁺, K⁺, Ca²⁺ tidak memberikan gangguan yang ketara pada analisis kuantitatif akrilamida, tetapi ion seperti Fe³⁺ and NH₃ telah mengganggu analisis ini.

Kata kunci: akrilamida, hidrolisis, reagen Nessler

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