Malaysian Journal of Analytical Sciences Vol 23 No 4 (2019): 572 - 579

AN OPTICAL SENSOR BASED ON GRAPHENE QUANTUM DOTS FOR HYDROGEN PEROXIDE DETECTION

(Sensor Optik berasaskan Titik Kuantum Grafin untuk Pengesanan Hidrogen Peroksida)

Amiruddin Ashil Mastar¹, Jaafar Abdullah^1,2*, Nor Azah Yusof^1,2, Yap Wing Fen^1,3

¹Department of Chemistry

²Institute of Advanced Technology

³Department of Physic, Faculty of Science

Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

*Corresponding author: jafar@upm.edu.my

Received: 19 August 2018; Accepted: 8 July 2019

Abstract

Graphene quantum dots (GQDs) is a zero-dimensional material of the carbon family and considered as a small cutting fragment from graphene sheet. It has unique electronic and optical properties due to electron confinement in the finite size of graphene cluster that leads to the opening of energy gap and quantization of electronic energy. In this study, biosensing based on GQDs in combination with enzyme (horseradish peroxidase, HRP) for the determination of hydrogen peroxide (H₂O₂) has been presented. The GQDs was used as an indicator reveals the fluorescence property of the system based on fluorescence quenching of GQDs which is induced from the enzymatic reaction. The presence of H₂O₂ quenches the fluorescence intensity of GQDs system which is proportional to the concentration of H₂O₂. Parameters optimization such as response time, enzyme concentrations, pH of buffer have been investigated. For linear calibration graph, it showed a linear dependence on the H₂O₂ concentration ranging from 1.0 to 100.0 μM with the detection limit of 1.0 μM.

Keywords: quantum dots, fluorescence, hydrogen peroxide, quenching, sensor

Abstrak

Titik kuantum grafin (GQDs) adalah bahan dimensi-sifar keluarga karbon dan dianggap sebagai serpihan kecil dari lembaran grafin. Ia mempunyai ciri-ciri elektronik dan optik yang unik disebabkan oleh pengasingan elektron dalam saiz finit kelas grafin yang membawa kepada pembukaan jurang tenaga dan pengkuantuman tenaga elektronik. Dalam kajian ini, biopenderiaan berasaskan GQDs digabungkan dengan enzim (peroksidase horseradish, HRP) untuk penentuan hidrogen peroksida (H₂O₂) telah dibentangkan. GQDs digunakan sebagai penunjuk mempamerkan sifat pendarfluor sistem berasaskan pendarfluor pelindapkejutan GQD yang diaruhkan dari tindak balas enzim. Kehadiran H₂O₂ mengurangkan keamatan pendarfluor sistem GQD yang berkadaran dengan kepekatan H₂O₂. Pengoptimuman parameter seperti masa tindak balas, kepekatan enzim, pH penimbal telah dikaji. Bagi graf penentukuran linear, ia menunjukkan kebergantungan kelinearan terhadap kepekatan H₂O₂ di antara 1.0 hingga 100.0 μM dengan had pengesanan 1.0 μM.

Katakunci: titik kuantum, pendarfluor, hidrogen peroksida, pelindapkejutan, sensor

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