Sains
Malaysiana 44(2)(2015): 261–267
Incidence
of Escherichia coli O157:H7 in Thailand
(Kejadian Escherichia coli O157:H7 di Thailand)
PHARANAI SUKHUMUNGOON*
Department
of Microbiology, Faculty of Science Prince of Songkla University
Hat-yai
90112, Thailand
Diserahkan:
7 Mei 2013/Diterima: 5 Ogos 2014
ABSTRACT
Enterohemorrhagic
Escherichia coli (EHEC)
especially serotype O157:H7 is
one of the important food-borne pathogens because it is able to
produce crucial toxins Shiga. However, the outbreak of this organism
in Thailand has not been reported. Antibody to O157 antigen was
detected in some Thai populations and Shiga toxin-producing E.
coli were detected in low numbers of clinical specimens. Interestingly,
some E. coli that showed positive to O157 fimbriae probe
and lack of virulence gene were isolated from certain patients and
one isolate of E. coli O157:H7
which possessed stx1,
stx2v was detected
in a normal child. In addition, the incidence of E. coli O157:H7
strains were monitored by the samples from cattle
and retail beef in Thailand although their inability to produce
toxins or produce in a low concentration was demonstrated. This
review discusses the incidences of E. coli O157 in clinical
and environmental samples of Thailand including the transmission
possibility of this bacterium across the Thai border through food
trade.
Keywords: E. coli O157:H7; EHEC; Malaysia; Thailand
ABSTRAK
Enterohemorrhagic
Escherichia coli (EHEC)
khususnya serotype O157:H7 adalah
salah satu patogen hasil makanan yang penting kerana ia dapat menghasilkan
toksin penting Shiga. Walau bagaimanapun, wabak organisma ini di
Thailand tidak dilaporkan. Antibodi kepada antigen O157 dikesan
dalam beberapa populasi Thai dan toksin Shiga yang menghasilkan
E. coli telah dikesan dalam bilangan yang rendah melalui
spesimen klinikal. Menariknya, terdapat beberapa E. coli yang
menunjukkan kesan positif kepada kuar fimbriae O157 dan kekurangan
gen virulen telah diasingkan daripada pesakit tertentu dan satu
pengasingan daripada E. coli O157:H7
yang mempunyai stx1,
stx2v dikesan pada
kanak-kanak biasa. Di samping itu, kadar strain E. coli O157:H7
telah dikawal melalui sampel daripada anak lembu
dan daging lembu runcit di Thailand walaupun ketidakmampuan mereka
untuk mengeluarkan toksin atau dalam kepekatan rendah telah ditunjukkan.
Kajian ini membincangkan kejadian E. coli O157 dalam sampel
klinikal dan alam sekitar di Thailand termasuk kemungkinan pemindahan
bakteria ini merentasi sempadan Thailand melalui perdagangan makanan.
Kata kunci: E. coli O157:H7; EHEC; Malaysia; Thailand
RUJUKAN
Bettelheim, K.A., Evangelidis, H., Pearce, J.L., Sowers, E. &
Strockbine, N.A. 1993. Isolation of Citrobacter freundii strain which
carries the Escherichia coli O157 antigen. Journal of Clinical
Microbiology 31(3): 760-761.
Bettelheim, K.A., Brown, J.E., Lolekha, S. & Echeverria, P. 1990.
Serotypes of Escherichia coli that hybridized with DNA probes for genes
encoding Shiga-like toxin I, Shiga-like toxin II, and serogroup O157
enterohemorrhagic E. coli fimbriae isolated from adults with diarrhea in
Thailand. Journal of Clinical Microbiology 28: 293-295.
Brown, J.E., Echeverria, P., Taylor, D.N., Seriwatana, J.,
Vanapruks, V., Lexomboon, U., Neill, R.N. & Newland, J.W. 1989.
Determination by DNA hybridization of Shiga-like-toxin-producing Escherichia
coli in children with diarrhea in Thailand. Journal of Clinical
Microbiology 27: 291-294.
Chart, H., Cheasty, T., Georgiou, T. & Rowe, B. 1993. Antigen
cross-reactions between Escherichia coli O157, Vibrio cholerae O1
(Inaba), and group N salmonella. Serodiagnosis and Immunotherapy in Infectious
Disease 5: 81-84.
Chomvarin, C., Ratchtrachenchai, O., Chantarasuk, Y., Srigulbutr,
S., Chaicumpar, K., Namwat, W. & Kotimanusvanij, D. 2005. Characterization
of diarrheagenic Escherichia coli isolated from food in Khon kaen,
Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health 36: 931-939.
Drasar, B.S. & Hill, M.J. 1974. Human Intestinal Flora. London:
Academic Press Ltd. pp. 36-43.
Dundas, S., Andrew Todd, W.T., Stewart, A.I., Murdoch, P.S.,
Chaudhuri, A.K.R. & Hutchinson, S.J. 2001. The central Scotland Escherichia
coli O157:H7 outbreak: Risk factors for the hemolytic uremic syndrome and
death among hospitalized patients. Clinical Infectious Diseases 33:
923-931.
Frampton, E.W., Restaino, L. & Blaszko, L. 1988. Evaluation of
the β-glucoronidase substrate 5-bromo-4-chloro-3-indolyl-
β-D-glucoronide (X-GLUC) in a 24-hour direct plating method for Escherichia
coli. Journal of Food Protection 51: 402-404.
Griffin, P.M. 1995. Escherichia coli O157:H7 and other
hemorrhagic Escherichia coli. In Infection of the Gastrointestinal
Tract, edited by Blaster, M.J., Smith, P.D. Ravdin, J.I., Greenberg, H.B.
& Guerrant, R.I. New York: Raven Press, Ltd. pp. 739-761.
Gupta, S., Soni, N.K., Kaur, P. & Sood, D.K. 1992.
Verocytopathic activity of Escherichia coli O157 and other ‘O’
serogroups isolated from patients of diarrhoea. Indian Journal of Medical
Research 95: 71-76.
Kalnauwakul, S., Phengmak, M., Kongmuang, U., Nakaguchi, Y. &
Nishibuchi, M. 2007. Examination of diarrheal stools in Hat Yai city, southern
Thailand, for Escherichia coli O157 and other diarrheagenic Escherichia
coli using immunomagnetic separation and PCR method. The Southeast Asian
Journal of Tropical Medicine and Public Health 38: 871-880.
Karmali, M.A., Steele, B.T., Petric, M. & Lim, C. 1983.
Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin
and cytotoxin-producing Escherichia coli in stools. Lancet. 1:
619-620.
Kim, Y.B., Okuda, J., Matsumoto, C., Morigaki, T., Asai, N.,
Watanabe, H. & Nishibuchi, M. 1998. Isolation of an Escherichia coli O157:H7
strain producing Shiga toxin 1 but not Shiga toxin 2 from a patient with
hemolytic uremic syndrome in Korea. FEMS Microbiology Letter 166: 43-48.
Koitabashi, T., Vuddhakul, V., Radu, S., Morigaki, T., Asai, N.,
Nakaguchi, Y. & Nishibuchi, M. 2006. Genetic characterization of Escherichia
coli O157:H7/- strains carrying the stx2 gene but not producing Shiga toxin 2. Microbiology and
Immunology 50: 135-148.
Leelaporn, A., Phengmak, M., Eampoklap, B., Manatsathit, S.,
Tritilanunt, S., Siritantikorn, S., Nagayama, K., Iida, T., Niyasom, C. &
Komolpit, P. 2003. Shiga toxin-and enterotoxin-producing Escherichia coli isolated
from subjects with bloody and nonbloody diarrhea in Bangkok, Thailand. Diagnostic
Microbiology and Infectious Disease 46: 173-180.
LeJeune, J.T., Abedon, S.T., Takemura, K., Christie, N.P. &
Sreevatsan, S. 2004. Human Escherichia coli O157:H7 genetic marker in
isolates of bovine origin. Emerging Infectious Diseases 10: 1482-1485.
Louise, C.B. & Obrig, T.G. 1995. Specific interaction of Escherichia
coli O157:H7-derived Shiga-like toxin II with human renal endothelial
cells. Journal of Infectious Diseases 172(5): 1397-1401.
Michino, H., Araki, K., Minami, S., Takaya, S., Sakai, N.,
Miyazaki, M., Ono, A. & Yanagawa, H. 1999. Massive outbreak of Escherichia
coli O157:H7 infection in schoolchildren in Sakai city, Japan, associated
with consumption of white radish sprouts. American Journal of Epidemiology 150:
787-796.
Minami, A., Chaicumpa, W., Chongsa-Nguan, M., Samosornsuk, S.,
Monden, S., Takeshi, K., Makino, S. & Kawamoto, K. 2010. Prevalence of
foodborne pathogens in open markets and supermarkets in Thailand. Food
Control 21: 221-226.
Muto, T., Matsumoto, Y., Yamada, M., Ishiguro, Y., Kitasume, S.,
Sasaki, K. & Toba, M. 2008. Outbreaks of enterohemorrhagic Escherichia
coli O157 infections among children with animal contact at a diary farm in
Yokohama city, Japan. Japanese Journal of Infectious Disease 61:
161-162.
Nataro, J.P. & Kaper, J.B. 1998. Diarrheagenic Escherichia
coli. Clinical Microbiology Review 11: 142-201.
Ooka, T., Terajima, J., Kusumoto, M., Iguchi, A., Kurokawa, K.,
Ogura, Y., Asadulghani, M., Nakayama, K., Murase, K., Ohnishi, M., Iyoda, S.,
Watanabe, H. & Hayashi, T. 2009a. Development of a multiplex PCR-based
rapid typing method for enterohemorrhagic Escherichia coli O157 strains. Journal of Clinical Microbiology 47: 2888-2894.
Ooka, T., Ogura, Y., Asadulghani, Md., Onishi, M., Nakayama, K.,
Terajima, J., Watanabe, H. & Hayashi, T. 2009b. Inference of the impact of
insertion sequence (IS) elements on bacterial genome diversification through
analysis of small-size structural polymorphisms in Escherichia coli O157
genomes. Genome Research 19: 1809-1816.
Rangel, J.M., Sparling, P.H., Crowe, C., Griffin, P.M. &
Swerdlow, D.L. 2005. Epidemiology of Escherichia coli O157:H7 outbreaks,
United States, 1982-2002. Emerging Infectious Diseases 11: 603-609.
Ratchtrachenchai, O.A., Subpasu, S., Hayashi, H. & Ba- Thein,
W. 2004. Prevalence of childhood diarrhoea-associated Escherichia coli in
Thailand. Journal of Medical Microbiology 53: 237-243.
Riley, L.W., Remis, R.S., Helgerson, S.D., McGee, H.B., Wells, J.G.,
Davis, B.R., Hebert, R.J., Olcott, E.S., Johnson, L.M., Hargrett,
N.T., Blake, P.A. & Cohen, M.L. 1983. Hemorrhagic colitis associated
with a rare Escherichia coli serotype. New England Journal
of Medicine 308: 681-685.
Sahilah, A.M., Nor'Aishah, H., Noraida, I. & Ahmas Azuhairi,
A. 2010. Detection of Shiga toxin 1 and 2 (stx1
and stx2)
genes in Escherichia coli O157:H7 isolated from retail beef
in Malaysia by multiplex polymerase chain reaction (PCR). Sains
Malaysiana 39(1): 57-63.
Seriwatana, J., Brown, J.E., Echeverria, P., Taylor, D.N.,
Suthienkul, O. & Newland, J. 1988. DNA probes to identify Shiga-like toxin
I-and II-producing enteric bacterial pathogens isolated from patients with
diarrhea in Thailand. Journal of Clinical Microbiology 26: 1614-1615.
Siegler, R.L. 1995. Hemolytic uremic syndrome in children. Current
Opinion in Pediatrics 7: 159-163.
Son,
R., Mutalib, S.A., Rusul, G., Ahmad, Z., Morigaki, T., Asai, N., Kim, Y.B.,
Okuda, J. & Nishibuchi, M. 1998. Detection of Escherichia
coli O157:H7 in the beef marketed in Malaysia. Applied and
Environmental Microbiology 64: 1153-1156.
Sukhumungoon,
P., Nakaguchi, Y., Ingviya, N., Pradutkanchana, J., Iwade, Y., Seto, K., Son,
R., Nishibuchi, M. & Vuddhakul, V. 2011a. Investigation of stx2+ eae+ Escherichia coli O157: H7 in beef
imported from Malaysia to Thailand. International Food Research Journal 18:
381-386.
Sukhumungoon,
P., Mittraparp-arthorn, P., Pomwised, R., Charernjiratragul, W. &
Vuddhakul, V. 2011b. High concentration of Shiga toxin 1-producing Escherichia
coli isolated from southern Thailand. International Food Research
Journal 18: 667-672.
Suthienkul,
O., Brown, J.E., Seriwatana, J., Thientongdee, S., Sastravaha, S. &
Echeverria, P. 1990. Shiga-like-toxin-producing Escherichia coli in
retail meats and cattle in Thailand. Applied and Environmental Microbiology 56:
1135-1139.
Voravuthikunchai,
S.P. 2002. Surveillance of enterohaemorrhagic Escherichia coli O157:H7
in Southern Thailand. Journal of Health, Population and Nutrition 20:
189-191.
Voravuthikunchai,
S.P., Chaowana, C., Perepat, P., Iida, T. & Honda, T. 2005. Antibodies
among healthy population of developing countries against enterohaemorrhagic Escherichia
coli O157:H7. Journal of Health, Population and Nutrition 23: 305-310.
Vuddhakul,
V., Patararungrong, N., Pungrasamee, P., Jitsurong, S., Morigaki, T., Asai, N.
& Nishibuchi, M. 2000. Isolation and characterization of Escherichia
coli O157 from retail beef and bovine feces in Thailand. FEMS Microbiol.
Letter 182: 343-347.
Yam,
W.C., Tsang, D.N., Que, T.L., Peiris, M., Seto, W.H. & Yuen, K.Y. 1998. A
unique strain of Escherichia coli O157:H7 that produces low
verocytotoxin levels not detected by use of a commercial enzyme immunoassay
kit. Clinical Infectious Diseases 27: 905-906.
Zadik,
P.M., Chapman, P.A. & Siddons, C.A. 1993. Use of tellurite for the
selection of verocytotoxigenic Escherichia coli O157. Journal of
Medical Microbiology 39: 155-158.
*Pengarang untuk
surat-menyurat; email: pharanai82@gmail.com
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