Sains Malaysiana 46(10)(2017): 1797–1805

http://dx.doi.org/10.17576/jsm-2017-4610-17

 

Kesan Penambahan Limonena terhadap Mikroemulsi Asid oleik/Cremophor rh 40/Transcutol/Air

(Effect of Limonene on the Oleic acid/Cremophor rh 40/Transcutol/Water Microemulsion)

 

ZAINUDDIN, N¹., AHMAD, I^1,2., ABDUL RAHMAN, I.³ & RAMLI, S.^1,2*

 

¹Pusat Pengajian Sains Kimia dan Teknologi Makanan, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan,Malaysia

 

²Pusat Penyelidikan Polimer (PORCE), Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

³Pusat Pengajian Fizik Gunaan, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

Diserahkan: 24 Januari 2017/Diterima: 10 Ogos 2017

 

ABSTRAK

Gambar rajah fasa pseudo-ternari sistem mikroemulsi asid oleik/Cremophor rh 40/Transcutol/Air diperoleh melalui pentitratan air pada nisbah surfaktan:kosurfaktan (Km) yang berbeza. Nisbah optimum bagi surfaktan/kosurfaktan adalah Km=2:1. Kesan penambahan limonena sebagai fasa minyak campuran terhadap sistem mikroemulsi diuji pada nisbah limonena:asid oleik (1:1, 2:1 dan 3:1). Penambahan limonena berupaya menghasilkan rantau mikroemulsi yang lebih besar sehingga 70%-80 % bt. air bagi kesemua nisbah limonena:asid oleik. Nisbah limonena:asid oleik (1:1) memberikan rantau mikroemulsi yang paling luas pada nisbah minyak:surfaktan/kosurfaktan (Minyak:S/KoS=1:9). Sifat isotropik mikroemulsi ditentukan dengan menggunakan mikroskopi cahaya polarasi. Mikroemulsi dibangunkan pada nisbah Km=2:1 dan Minyak:S/KoS (1:9) serta limonena:asid oleik (1:1). Kestabilan dan saiz partikel bagi sistem dikaji dan penambahan limonena didapati tidak merubah sifat serta mikro-struktur sistem mikroemulsi. Kajian konduktiviti elektrik dan kelikatan sistem menunjukkan pembentukan mikroemulsi jenis air-dalam-minyak (10% dan 20 % bt. air) dan dwiselanjar (30%-50 % bt. air). Kesemua sistem mempunyai potensi sebagai sistem penghantar bahan aktif dan menunjukkan kestabilan yang baik pada suhu 4, 25 dan 37°C dalam tempoh lebih daripada 6 bulan.

 

Kata kunci: Fasa mikroemulsi; gambar rajah fasa; limonena; mikroemuls; penghantar bahan aktif

 

ABSTRACT

The pseudo-ternary phase diagram of oleic acid/cremophor rh 40/transcutol/water was prepared along the water dilution line at different ratio of surfactant to cosurfactant (Km). The optimum ratio of surfactant to surfactant was Km=2:1. The effect of addition limonene as a mixed oils phase on the system were done with the ratio of oleic acid to limonene at 1:1, 1:2 and 1:3. Addition of limonene is able to form stable microemulsions up to 70-80 wt. % water content for all ratios of oleic acid to limonene. The ratio of limonene:oleic acid (1:1) resulted on the largest microemulsion region was obtained at the ratio of oil:surfactant/cosurfactant (Oil:S/CoS=1:9). The isotropic properties of microemulsion phases were confirmed by polarized light microscopy. Both systems were prepared at Km=2:1 and 1:9 (oil:S/CoS) as well as 1:1 (limonene:oleic acid). The stability and particle size study was performed for both systems and its show that the addition of limonene does not change the properties and microstructure of the system. Electrical conductivity and viscosity study of the systems reveals that transformation from water-in-oil microemulsion (10% and 20 wt. % water) and bicontinuous microemulsion (30% to 50 wt. % water). All microemulsions prepared may have potentials as a drug delivery system and show high stability for over 6 months at 4, 25 and 37°C.

 

Keywords: Drug delivery; limonene; microemulsion; microemulsion phase; phase diagram

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*Pengarang untuk surat-menyurat; email: su_ramli@ukm.edu.my