Sains Malaysiana 51(11)(2022): 3689-3702

http://doi.org/10.17576/jsm-2022-5111-14

 

Sintesis dan Pencirian Kompleks Fenolbenzimidazol dan Kajian Interaksi terhadap CT-DNA

(Synthesis and Characterization of Phenolbenzimidazole Complexes and Interaction Studies with CT-DNA)

 

NOR FALAHIAH ISMAIL, NUR AQILAH ZAHIRAH NORAZMI & NURUL HUDA ABD KARIM^*

 

Department of Chemical Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

 

Diserahkan: 30 Mac 2022/Diterima: 12 Julai 2022

 

Abstrak

Kompleks fenolbenzimidazol berjaya disintesis dan dicirikan menggunakan spektroskopi resonans magnet nukleus (RMN), spektroskopi inframerah transformasi Fourier (FTIR), penentuan julat takat lebur, analisis ultralembayung-boleh nampak (UL-BN), analisis unsur (CHNS), kerentanan magnet dan Kristalografi Sinar-X. Mod pengikatan interaksi antara kompleks fenolbenzimidazol dengan DNA timus anak lembu (CT-DNA) dikaji dengan kaedah pentitratan DNA menggunakan serapan elektronik UL-BN dan kajian nisbah kepekatan DNA menggunakan analisis pendarfluor. Berdasarkan spektrum UL-BN, anjakan hipokromism dan batokromism diperhatikan apabila kompleks fenolbenzimidazol dititratkan dengan CT-DNA dan menunjukkan pembentukan kompleks baru. Kompleks fenolbenzimidazol berinteraksi dengan CT-DNA secara pengikatan interkalasi dengan pemalar pengikatan, K_b (1.68 ± 1.4) × 10⁵ - (1.02 ± 0.1) × 10⁷ M^-1. Kajian pendarfluor dijalankan untuk memerhati sifat optik kompleks fenolbenzimidazol terhadap CT-DNA. Keputusan kajian ini sangat bernilai dan berpotensi dalam menjadikan kompleks fenolbenzimidazol sebagai prob DNA sensor optik.

 

Kata kunci: DNA timus; interkalasi; kompleks fenolbenzimidazol

 

Abstract

Phenolbenzimidazole complexes were successfully synthesized, and characterizations were carried out using Nuclear Magnetic Resonance Spectroscopy (NMR), Infrared Spectroscopy (FTIR), melting point range determination, ultraviolet-visible (UV-Vis) analysis, elemental analysis CHNS, magnetic susceptibility and X-ray crystallography.  The binding modes of interaction between phenolbenzimidazole complexes and calf thymus (CT-DNA) were determined by UV-Vis titration and fluorescence using distinct ratios. Based on UV-Vis spectra, hypochromism and bathochromism (red-shift) were observed upon titration of the complexes with CT-DNA which suggested formation of new complexes. Phenolbenzimidazole complexes interact with CT-DNA by intercalation binding mode with the binding constant, K_b (1.68 ± 1.4) × 10⁵ - (1.02 ± 0.1) × 10⁷ M^-1. Fluorescence study was performed to observe the optical properties of phenolbenzimidazole complexes against CT-DNA. These findings will be valuable for the potential use of phenolbenzimidazole complex as a probe in optical sensor DNA.  

 

Keywords: Intercalation; phenolbenzimidazole complexes; thymus DNA

 

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^*Pengarang untuk surat-menyurat; email: nurulhuda@ukm.edu.my