Sains Malaysiana 52(1)(2023): 153-164

http://doi.org/10.17576/jsm-2023-5201-12

 

Optimization of Ultrasound-Assisted Extraction for Antioxidant Activity in Relation to Rhoifolin Content of Fortunella polyandra using Response Surface Methodology (RSM)

(Pengoptimuman Pengekstrakan Berbantu Ultrabunyi untuk Aktiviti Antioksidan Berkaitan Kandungan Rhoifolin Fortunella polyandra menggunakan Kaedah Gerak Balas Permukaan (RSM))

 

HAZIM SYAHMI ELIAS1,3, SYARUL NATAQAIN BAHARUM1, KAMALRUL AZLAN AZIZAN1, WAN YAACOB WAN AHMAD2, SHEELA CHANDREN3 & NORAZAH BASAR3,4,*

 

1Metabolomics Research Laboratory, Institute of Systems Biology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

2School of Chemical Science and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

3Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor Darul Takzim, Malaysia

4Centre of Lipids Engineering & Applied Research (CLEAR), Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor Darul Takzim, Malaysia

 

Diserahkan: 5 Julai 2022/Diterima: 4 November 2022

 

Abstract

Fortunella polyandra (Ridl) Tanaka or Malayan kumquat, is a family of Rutaceae from Malaysia known for its various active compounds, especially flavonoids. This study aims to verify the optimum condition and correlation of leaves extract in Fortunella polyandra by ultrasound-assisted extraction (UAE) for antioxidant activities and apigenin 7-O-neohesperidoside (rhoifolin) contents using the response surface methodology (RSM). Central composite rotatable design (CCD) was performed to investigate the influence of the extraction parameters on maximum antioxidant activity, namely temperatures (X1), times (X2), and solvent ratios of ethanol:water (X3). The optimal extraction conditions for 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were obtained at extraction time of 5 min, temperature of 30 °C and solvent ratio of 70:30, ethanol:water with IC50 value of  0.126±0.004 mg/mL. Rhoifolin content and ferric reducing antioxidant potential (FRAP) assay share the same optimum condition at extraction time of 5 min, temperature of 30 °C, and solvent ratio of 30:70, ethanol:water. Under these conditions, rhoifolin content was measured at 67.83±0.37 ppm, whereas FRAP equivalent value was recorded at 0.22±0.01 mg/mL. All three responses from the model achieved 95% confidence level. Correlation study showed that there is no relationship between the rhoifolin content and the antioxidant activities of Fortunella polyandra extract.

 

Keywords: Antioxidant activity; central composite design (CCD); Fortunella polyandra; response surface methodology (RSM); rhoifolin; ultrasound-assisted extraction (UAE)

 

Abstrak

Fortunella poliandra(Ridl) Tanaka atau kumquat Malaya, tergolong dalam famili Rutaceae dari Malaysia yang terkenal dengan pelbagai sebatian aktif terutamanya flavonoid. Walau bagaimanapun, kaedah pengekstrakan tradisi yang digunakan untuk mengekstrak sebatian aktif ini mengambil masa yang lama dan rumit. Secara khususnya, kaedah pengekstrakan berbantukan ultrabunyi (UAE) untuk mengekstrak sebatian aktif telah diketengahkan dan boleh menjadi salah satu kaedah yang berkesan untuk mengekstrak sebatian aktif daripada Fortunella polyandra. Kajian ini bertujuan untuk menentusahkan keadaan optimum ekstrak daun Fortunella poliandra menggunakan kaedah pengekstrakan berbantu ultrabunyi (UAE) untuk mengkaji aktiviti antioksidan dan korelasi kandungan apigenin 7-O-neohesperidosida (rhoifolin) menggunakan kaedah gerak balas permukaan(RSM). Reka bentuk komposit pusat (CCD) digunakan untuk mengkaji kesan parameter pengekstrakan ke atas aktiviti optimum antioksidan, iaitu suhu (X1), masa (X2) dan nisbah pelarut etanol:air (X3). Keadaan optimum pengekstrakan untuk aktiviti pemerangkapan 2,2-difenil-1-pikrillhidrazil (DFPH) diperoleh pada masa pengekstrakan 5 minit, suhu 30 °C dan nisbah pelarut 70:30, etanol:air dengan nilai IC50 0.126±0.004 mg/mL. Kandungan rhoifolin dan cerakinan keupayaan antioksidan penurunan ferik (FRAP) menunjukkan keadaan optimum yang sama iaitu pada masa pengekstrakan selama 5 minit, suhu 30 °C dan nisbah pelarut 30:70, etanol:air. Di bawah keadaan optimum ini, kandungan rhoifolin yang diperoleh adalah 67.83±0.37 ppm, manakala nilai setara FRAP direkodkan pada 0.22±0.01 mg/mL. Ketiga-tiga gerak balas daripada model ini mencapai aras keyakinan 95%. Kajian korelasi menunjukkan tiada hubungan antara kandungan rhoifolin dan aktiviti antioksidan ekstrak Fortunella poliandra.

 

Kata kunci: Aktiviti antioksidan; Fortunella poliandra; kaedah gerak balas permukaan (RSM); pengekstrakan berbantu ultrabunyi (UAE); reka bentuk komposit pusat (CCD); rhoifolin

 

RUJUKAN

Abd-Elhady El-gizawy, H. & Hussein, M. 2016. Isolation, structure elucidation of ferulic and coumaric acids from Fortunella japonica Swingle leaves and their structure antioxidant activity relationship. Free Radicals and Antioxidants 7: 23-30.

Barreca, D., Bellocco, E., Caristi, C., Leuzzi, U. & Gattuso, G. 2011. Kumquat (Fortunella japonica Swingle) juice: Flavonoid distribution and antioxidant properties. Food Research International 44: 2190-2197.

Benzie, I.F. & Strain, J.J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry 239(1): 70-76.

Çakmakçi, S., Topdaş, E.F., Çakir, Y. & Kalin, P. 2016. Functionality of kumquat (Fortunella margarita) in the production of fruity ice cream. Journal of the Science of Food and Agriculture 96(5): 1451-1458.

Cavazzuti, M. 2013. Optimization Methods: From Theory to Design Scientific and Technological Aspects in Mechanics. 2013th ed. Springer.

Hou, W., Zhang, W., Chen, G. & Luo, Y. 2016. Optimization of extraction conditions for maximal phenolic, flavonoid and antioxidant activity from Melaleuca bracteata leaves using the response surface methodology. PLoS ONE 11(9): 1-11.

Jing, C.L., Dong, X.F. & Tong, J.M. 2015. Optimization of ultrasonic-assisted extraction of flavonoid compounds and antioxidants from alfalfa using response surface method. Molecules 20: 15550-15571.

Kassim, N.K., Rahmani, M., Ismail, A., Sukari, M.A., Ee, G.C., Nasir, N.M. & Awang, K. 2013. Antioxidant activity-guided separation of coumarins and lignan from Melicope glabra (Rutaceae). Food Chemistry 15(139): 1-4.

Kitagawa, T., Matsumoto, T., Imahori, D., Kobayashi, M., Okayama, M., Ohta, T., Yoshida, T. & Watanabe, T. 2021. Limonoids isolated from the Fortunella crassifolia and the Citrus junos with their cell death-inducing activity on Adriamycin-treated cancer cell. Journal of Natural Medicines 75(4): 998-1004.

Liu, X.F. 2014. Optimization of total flavonoids extraction from Coreopsis tinctoria Nutt. by response surface methodology. Journal of Chemists and Chemical Engineers 63(11-12): 391-396.

Lee, L.S., Lee, N., Kim, Y.H., Lee, C.H., Pil, H.S., Jeon, Y.W. & Kim, Y.E. 2013. Optimization of ultrasonic extraction of phenolic antioxidants from green tea using response surface methodology. Molecules 18: 13530-13545.

Li, H.Z., Zhang, Z.J., Xue, J., Cui, L.X., Hou, T., Li, X.J. & Chen, T. 2016. Optimization of ultrasound-assisted extraction of phenolic compounds, antioxidants and rosmarinic acid from Perilla leaves using response surface methodology. Food Science and Technology 36(4): 686-693.

Lou, S.N. & Ho, C. 2016. Phenolic compounds and biological activities of small-size citrus: Kumquat and calamondin. Journal of Food and Drug Analysis 25(1): 162-175.

Lou, S.N., Lai, Y.C., Huang, J.D., Ho, C.T., Ferng, L.H.A. & Chang, Y.C. 2015. Drying effect on flavonoid composition and antioxidant activity of immature kumquat. Food Chemistry 171: 356-363.

Owolabi, R.U., Usman, M.A. & Kehinde, A.J. 2018. Modelling and optimization of process variables for the solution polymerization of styrene using response surface methodology. Journal of King Saud University - Engineering Sciences 30(1): 22-30.

Rai, A., Mohanty, B. & Bhargava, R. 2015. Supercritical extraction of sunflower oil: A central composite design for extraction variables. Food Chemistry 192: 647-659.

Tan, S., Li, M., Ding, X., Fan, S., Guo, L., Gu, M., Zhang, Y., Feng, L., Jiang, D., Li, Y., Xi, W., Huang, C. & Zhou, Z. 2014. Effects of Fortunella margarita fruit extract on metabolic disorders in high-fat diet-induced obese C57BL/6 mice. PLoS ONE 9(4): e93510.

Tan, S., Zhao, X., Yang, Y., Ke, Z. & Zhou, Z. 2016. Chemical profiling using Uplc Q-Tof/Ms and antioxidant activities of fortunella fruits. Journal of Food Science 81(7): C1646-C1653.

Weremfo, A., Abassah-Oppong, S., Adulley, F., Dabie, K. & Seidu-Larry, S. 2022. Response surface methodology as a tool to optimize the extraction of bioactive compounds from plant sources. Journal of the Science of Food and Agriculture 103(1): 26-36.

 Xu, D.P., Zheng, J., Zhou, Y., Li, Y., Li, S. & Li, H.B. 2016. Extraction of natural antioxidants from the Thelephora ganbajun mushroom by an ultrasound-assisted extraction technique and evaluation of antiproliferative activity of the extract against human cancer cells. International Journal of Molecular Sciences 17(10): 1664.

Xu, D., Zhou, Y., Zheng, J., Li, S., Li, A.N. & Li, H.B. 2015. Optimization of ultrasound-assisted extraction of natural antioxidants from the flower of Jatropha integerrima by response surface methodology. Molecules 21(1): 18.

Zhang, L., Jiang, Y., Pang, X., Hua, P., Gao, X., Li, Q. & Li, Z. 2019. Simultaneous optimization of ultrasound-assisted extraction for flavonoids and antioxidant activity of Angelica keiskei using response surface methodology (RSM). Molecules 24(19): 3461.

Zhao, Y., Hou, Y., Tang, G., Cai, E., Liu, S., Yang, H., Zhang, L. & Wang, S. 2014. Optimization of ultrasonic extraction of phenolic compounds from Epimedium brevicornumMaxim using response surface methodology and evaluation of its antioxidant activities in vitro. Journal of Analytical Methods in Chemistry 2014: 1-7.

 

*Pengarang untuk surat-menyurat; email: norazahb@utm.my

 

   

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