Sains Malaysiana 52(1)(2023): 175-185

http://doi.org/10.17576/jsm-2023-5201-14

Evaluation of Xylose-Utilising Yeasts for Xylitol Production from Second-Generation Ethanol Vinasse and Effect of Agitation Intensity in Flask-Scale Xylitol Production

(Penilaian Yis mengguna Xilosa untuk Pengeluaran Xilitol daripada Vinasse Etanol Generasi Kedua dan Kesan Keamatan Pergolakan dalam Pengeluaran Xilitol Skala Flask)

SREYDEN HOR^1,2, MALLIKA BOONMEE KONGKEITKAJORN^2,3,* & ALISSARA REUNGSANG^2,4

¹Graduate School, Khon Kaen University, Khon Kaen, Thailand

²Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, Thailand

³Research Center for Environmental and Hazardous Substance Management (EHSM), Khon Kaen University, Khon Kaen, Thailand

⁴Academy of Science, Royal Society of Thailand, Bangkok, Thailand

Diserahkan: 5 Jun 2022/Diterima: 5 Oktober 2022

Abstract

This study aimed to select a yeast strain that effectively utilises xylose to produce xylitol from the vinasse of ethanol broth obtained from the fermentation of sugarcane bagasse hydrolysate. Eleven strains of xylose-fermenting yeasts were evaluated for their abilities to utilise xylose and produce xylitol. Two strains that showed outstanding performance in the semi-defined xylose medium were selected for further testing with a vinasse medium. Candida guilliermondii TISTR 5068 showed a superior xylitol production of 7.03 ± 0.08 g/L with the xylitol yield of 0.70 g/g_xylose when cultured in bagasse-based ethanol vinasse. The strain was further tested for its xylitol production performance when cultured at four different agitation intensities. Excessive agitation resulted in a rapid xylitol production rate but caused xylitol consumption once the xylose was depleted. Moderate agitation resulted in the highest xylitol yield of 0.79 g/g_xylose. The results of this study have provided important information for the development of the xylitol production process using waste streams from cellulosic ethanol production.

Keywords: Aeration; biorefineries; fermentation; vinasse; xylitol; yeasts

Abstrak

Kajian ini bertujuan untuk memilih strain yis yang menggunakan xilosa secara berkesan untuk menghasilkan xilitol daripada vinasse rebusan etanol yang diperoleh daripada penapaian hidrolisat hampas tebu. Sebelas strain yis difermentasi xilosa dinilai untuk kebolehan mereka untuk menggunakan xilosa dan menghasilkan xilitol. Dua strain yang menunjukkan prestasi cemerlang dalam medium xilosa separa takrif telah dipilih untuk ujian selanjutnya dengan medium vinasse. Candida guilliermondii TISTR 5068 menunjukkan pengeluaran unggul xilitol sebanyak 7.03 ± 0.08 g/L dengan hasil xilitol sebanyak 0.70 g/g_xylose apabila dikultur dalam vinasse etanol berasaskan hampas. Strain diuji lagi untuk prestasi pengeluaran xilitol apabila dikultur pada empat keamatan pengadukan yang berbeza. Penggoncangan yang berlebihan mengakibatkan kadar pengeluaran xilitol yang cepat tetapi menyebabkan penggunaan xilitol sebaik sahaja xilosa habis. Penggoncangan sederhana menghasilkan hasil xilitol tertinggi iaitu 0.79 g/g_xylose. Hasil kajian ini telah memberikan maklumat penting untuk pembangunan proses penghasilan xilitol menggunakan aliran sisa daripada penghasilan etanol selulosa.

Kata kunci: Fermentasi; kilang penapisan bio; pengudaraan; vinasse; xilitol; yis

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^*Pengarang untuk surat-menyurat; email: mallikab@kku.ac.th