Sains
Malaysiana 52(2)(2023): 513-531
http://doi.org/10.17576/jsm-2023-5202-15
UHPLC-Q-Orbitrap
HRMS-Based
Metabolomic Show Biological Pathways Involved in Rice (Oryza sativa L.)
under Fe Toxicity Stress
(Metabolomik UHPLC-Q-Orbitrap Berasaskan HRMS menunjukkan
Laluan Biologi Terlibat untuk Beras (Oryza
sativa L.) di bawah Tekanan Ketoksikan Fe)
TURHADI TURHADI1,2,
HAMIM HAMIM3, MUNIF GHULAMAHDI4 & MIFTAHUDIN
MIFTAHUDIN3,*
1Plant Biology Graduate Program, Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Kampus IPB Dramaga 16680 Bogor, West Java, Indonesia
2Department
of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya,
Jl. Veteran, Malang 65415, East Java, Indonesia
3Department
of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Kampus
IPB Dramaga 16680 Bogor, West Java, Indonesia
4Department
of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Kampus
IPB Dramaga 16680 Bogor, West Java, Indonesia
Diserahkan: 19 Julai 2022/Diterima: 30 November 2022
Abstract
The iron (Fe)
toxicity stress is still a serious problem in
rice cultivation, especially on land with high Fe content. The Fe toxicity stress affects
various complex physiological aspects of plants. The metabolomic analysis
using LC-MS is expected to provide information about rice's metabolism regulation under Fe toxicity stress. The objective of this study was to show the biological pathway signature in
rice after exposure to Fe toxicity stress using
UHPLC-Q-Orbitrap HRMS-based metabolomic analysis. The two rice varieties, i.e., IR64 (Fe-sensitive) and Pokkali (Fe-tolerant) were analyzed their metabolites using UHPLC-Q-Orbitrap HRMS. The metabolite profiles of both
varieties were analyzed using MetaboAnalyst 5.0 software. The results showed that Fe toxicity stress affected
the metabolite profile in both root and shoot tissues of two rice varieties. A number of 102 metabolites were detected in root and shoot tissues of rice. The comprehensive univariate and multivariate analyses
showed that 1-aminocyclopropane-1-carboxylate (ACC) in shoot tissues and
galactose in root tissues was suggested as metabolite markers for Fe tolerance
character of rice var. Pokkali. The
genes encoded the enzymes involved in biosynthetic pathway of both metabolite
markers could be a target to be explored for Fe toxicity tolerance in rice.
Keywords: Galactose;
metabolism; metabolite markers; 1-aminocyclopropane-1-carboxylate
Abstrak
Tekanan ketoksikan besi (Fe) masih menjadi masalah serius dalam penanaman
padi, terutamanya pada tanah yang mempunyai kandungan Fe yang tinggi. Tekanan ketoksikan Fe mempengaruhi pelbagai aspek fisiologi tumbuhan yang
kompleks. Analisis metabolomik menggunakan LC-MS dijangka
memberikan maklumat tentang peraturan metabolisme beras di bawah tekanan
ketoksikan Fe. Objektif kajian ini adalah untuk menunjukkan pengenalan
laluan biologi dalam beras selepas terdedah kepada tekanan ketoksikan Fe
menggunakan analisis metabolomik berasaskan UHPLC-Q-Orbitrap HRMS. Kedua-dua varieti beras, iaitu IR64 (Fe-sensitif) dan Pokkali
(Fe-toleransi) telah dianalisis metabolitnya menggunakan UHPLC-Q-Orbitrap HRMS. Profil metabolit kedua-dua jenis varieti dianalisis menggunakan perisian
MetaboAnalyst 5.0. Keputusan
menunjukkan bahawa tekanan ketoksikan Fe mempengaruhi profil metabolit dalam
kedua-dua tisu akar dan pucuk kedua-dua varieti padi. Sejumlah 102 metabolit telah dikesan dalam tisu akar dan pucuk padi. Analisis komprehensif univariat dan multivariat menunjukkan bahawa
1-aminosiklopropana-1-karboksilat (ACC) dalam tisu pucuk dan galaktosa dalam
tisu akar telah dicadangkan sebagai penanda metabolit untuk sifat toleransi Fe
bagi beras var. Pokkali. Gen yang mengekod
enzim yang terlibat dalam laluan biosintetik kedua-dua penanda metabolit boleh
menjadi sasaran untuk diterokai untuk toleransi ketoksikan Fe dalam beras.
Kata kunci: Galaktosa; metabolisme; penanda metabolit; 1-aminosiklopropana-1-karboksilat
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*Pengarang untuk
surat-menyurat; email: miftahudin@apps.ipb.ac.id
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