Sains Malaysiana 52(4)(2023): 1161-1171

http://doi.org/10.17576/jsm-2023-5204-10

Gibberellic Acid Supplements Mitigate the Sodium Chloride Effects on Onion Seed Germination and Its Physio-Chemical Attributes

(Suplemen Asid Giberelik Mengurangkan Kesan Natrium Klorida pada Percambahan Biji Bawang dan Atribut Fisio-Kimianya)

IKRAM-UL HAQ¹, NAZIA PARVEEN GILL², GHULAM YASIN³, SAHIFA MEMON¹, ZAINAB SOOMRO¹, FAHEEM AHMED BHATTI^1,4, MEHWISH BALOCH¹ & CHANDA BAKHTAWAR BALOCH¹

¹Institute of Biotechnology and Genetic Engineering (IBGE), University of Sindh, Jamshoro-76080, Pakistan

²Department of Statistics, University of Sindh, Jamshoro-76080, Pakistan

³Department of Botany, Bahauddin Zakariya University, Multan-60800, Pakistan

⁴Sindh Agriculture Department, Tandojam-70060, Pakistan

Diserahkan: 3 Julai 2022/Diterima: 17 Mac 2023

Abstract

The mitigative effect of gibberellic acid (GA₃) for salt (NaCl) stresses on seed germination attributes of onion (Allium cepa L.) cultivar Nasarpuri was assessed. Seeds were moisturized with NaCl (0-, 100- and 200-mM) and GA₃ (250 ppm) before and after sowing for seed germination in 1^st week than in 2^nd week, GA₃ sprayed once foliarly and NaCl in rooting region. At the end of 1^st week, an increase in seed germination rate was observed in seeds supplemented with GA₃ from control (85.0%) to 97.5% and from NaCl stressed seeds (72.5% and 50.0%) to 85.0% and 62.5%, respectively (p ≤ 0.05). This reduction in seed germination was caused by salt stresses after 96th hours of sowing, inhibition in GA₃-biosynthesis GA₃ and delay in α-amylases activation observed in salt stressed seed cultures. The seedling vigor index (SVI) was observed higher in foliarly GA₃ sprayed cultures of both control as well as saline stressed cultures. The seedlings supplemented with GA₃, decrease in malondialdehyde (MDA), H₂O₂, Na⁺/K⁺, Na⁺ and Cl^- contents, while increases seedling biomass, chlorophyll contents, total proteins, and sugars in NaCl stressed seedlings. Interestingly, GA₃ also increased (p ≤ 0.05) the osmoprotectants in seedlings including abscisic acid (AsA), carotenoids, phenolics and proline contents to depict in stress alleviation. This study may be concluded by the fact that GA₃ minimizes salinity stresses on seed germination as well as further seedling growth with the increased production of organic osmoprotectants as saline stress neutralizers.

Keywords: Allium cepa L.; antioxidative responses; gibberellic acid (GA₃); H₂O₂ contents; saline stresses

Abstrak

Kesan mitigasi asid giberelik (GA₃) untuk garam (NaCl) menegaskan sifat percambahan benih bawang (Allium cepa L.) kultivar Nasarpuri telah dinilai. Benih telah dilembapkan dengan NaCl (0-, 100- dan 200-mM) dan GA₃ (250 ppm) sebelum dan selepas disemai untuk percambahan benih pada minggu pertama berbanding minggu ke-2, GA₃ disembur sekali pada daun dan NaCl di kawasan pengakaran. Pada akhir minggu pertama, peningkatan kadar percambahan biji benih diperhatikan pada benih yang ditambah dengan GA₃ daripada kawalan (85.0%) kepada 97.5% dan daripada benih bertekanan NaCl (72.5% dan 50.0%) kepada 85.0% dan 62.5% masing-masing (p). ≤ 0.05). Pengurangan dalam percambahan benih ini disebabkan oleh tegasan garam selepas 96 jam penyemaian, perencatan dalam GA₃-biosintesis GA₃ dan kelewatan dalam pengaktifan α-amilase yang diperhatikan dalam kultur benih bertekanan garam. Indeks vigor ankak benih (SVI) diperhatikan lebih tinggi dalam kultur semburan GA₃ daun bagi kedua-dua kawalan dan juga kultur bertekanan garam. Anak benih yang ditambah dengan GA₃ mengurangkan kandungan malondialdehid (MDA), H₂O₂, Na+/K+, Na+ dan Cl-, sambil meningkatkan biojisim anak benih, kandungan klorofil, jumlah protein dan gula pada anak pokok bertekanan NaCl. Menariknya, GA₃ juga meningkatkan (p ≤ 0.05) osmopelindung dalam anak benih termasuk kandungan asid absisik (AsA), karotenoid, fenol dan prolin untuk menggambarkan pengurangan tekanan. Kajian ini boleh disimpulkan oleh fakta bahawa GA₃ meminimumkan tegasan kemasinan pada percambahan biji benih serta pertumbuhan anak benih selanjutnya dengan peningkatan pengeluaran osmopelindung organik sebagai peneutral tegasan garam.

Kata kunci: Allium cepa L.; asid giberelik (GA₃); gerak balas antioksidatif; kandungan H₂O₂, tegasan garam

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