Malaysian
Journal of Analytical Sciences Vol 20 No 1 (2016): 1 - 13
ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF LICHENS Usnea rubrotincta, Ramalina dumeticola, Cladonia
verticillata AND THEIR CHEMICAL CONSTITUENTS
(Kajian Aktiviti
Antibakteria dan Antioksidan Liken Usnea
rubrotincta, Ramalina dumeticola, Cladonia verticillata dan
Sebatian Kimianya)
Saranyapiriya
Gunasekaran1*, Vinoshene Pillai Rajan1, Surash Ramanathan2,
Vikneswaran Murugaiyah3,
Mohd. Wahid Samsudin1, Laily B Din1
1School of Chemical Science and Food Technology,
Faculty of Science and Technology
Universiti
Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
2Centre
for Drug Research
3School
of Pharmaceutical Sciences
Universiti
Sains Malaysia, 11800 Gelugur, Penang, Malaysia
*Corresponding author: saranyapiriya89@gmail.com
Received: 24
August 2015; Accepted: 4 November 2015
Abstract
The present
study was carried out to evaluate the antibacterial and antioxidant activity of
extract and chemical constituents of Usnea rubrotincta, Ramalina
dumeticola and Cladonia verticillata.
Acetone extract of U. rubrotincta
showed promising antibacterial
activity against Gram positive bacteria Bacillus
subtilis with the lowest Minimal Inhibitory Concentration (MIC) value of
(15.63 µg/mL). Six secondary metabolites were isolated using Preparative High
Performance Liquid Chromatography (PHPLC) method from the two bioactive lichens
U. rubrotincta and R. dumeticola (compound 1 – 6). Among
all six compounds, compound (1) exhibited strongest activity against both the
tested Gram positive bacteria at 7.81 µg/mL. Compound (6) had 69.57% scavenging
activity against DPPH free radical while the rest only showed below 50%
scavenging activity. This is the first evaluation of antibacterial activity of
lichens found in Malaysia and to our knowledge, this is the first report of
antibacterial and antioxidant activity of compound (3) and (5).
Keywords: antibacterial, antioxidant, Usnea rubrotincta, Ramalina dumeticola, Cladonia verticillata, usnic acid
Abstrak
Kajian
ini dijalankan bagi menilai aktiviti antibakteria dan antioksidan sebatian
kimia liken yang diekstrak iaitu Usnea
rubrotincta, Ramalina dumeticola
dan Cladonia verticillata. Ekstrak
aseton terhadap U. rubrotincta telah menunjukkan aktiviti antibakteria yang
memberangsangkan terhadap bakteria Gram positif Bacillus subtilis dengan nilai Kepekatan Perencatan Minimum (KPM)
sebanyak (15.63 µg/mL). Enam metabolit sekunder telah dipencilkan daripada 2
liken bioaktif iaitu U. rubrotincta
dan R. dumeticola menggunakan kaedah
Kromatografi Cecair Prestasi Tinggi Preparatif (KCPTP) (sebatian 1 – 6).
Daripada 6 sebatian, sebatian (1) telah memaparkan aktiviti yang paling kuat
terhadap kedua-dua bakteria Gram positif yang dikaji pada kepekatan 7.81 µg/mL.
Sebatian (6) mempunyai peratus pemerangkapan radikal bebas DPPH pada 69.57%
manakala sebatian lain hanya menunjukkan aktiviti pemerangkapan kurang daripada
50%. Ini adalah penilaian pertama terhadap aktiviti antibakteria bagi liken yang
dijumpai di Malaysia dan kepada pengetahuan kita, ini adalah laporan pertama
bagi aktiviti antibakteria dan antioksidan sebatian (3) dan (5).
Kata kunci: antibakteria,
antioksidan, Usnea rubrotincta, Ramalina
dumeticola, Cladonia verticillata, asid usnik
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