Malaysian Journal of Analytical
Sciences Vol 20 No 1 (2016): 111 - 120
SYNTHESIS AND CHARACTERIZATION OF 6,6’-BIS(2-HYDROXYPHENYL)-2,2’-BIPYRIDYL LIGAND AND
ITS PLATINUM COMPLEX FOR THE INTERACTION WITH CT-DNA
(Sintesis dan Pencirian
Ligan 6,6’-Bis(2-Hidroksifenil)-2,2’-Bipiridil dan Kompleks Platinum untuk
Kajian Interaksi dengan CT-DNA)
Norhidayah Selamat,
Lee Yook Heng, Nurul Izzaty Hassan, Nurul Huda Abd Karim*
School
of Chemical Sciences and Food Technology,
Faculty
of Sciences and Technology,
Universiti
Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
*Corresponding author:
nurulhuda@ukm.edu.my
Abstract
A tetradentate
ligand with four donor atoms OONN and
its platinum metal complex were synthesized. Bis(phenoxy)bipyridine
ligand was prepared by Suzuki coupling reaction between
6,6’-dibromo-2,2’-bipyridyl and 2-hydroxyphenylboronic acid with the presence
of palladium (II) acetate. The formation of platinum complex was done by
introducing the ligand with platinum (II) chloride in benzonitrile. Both ligand
and complex structures were confirmed by 1H, 2D cosy and 13C
NMR spectroscopy, ESI-MS spectrometry and FTIR spectroscopy. Binding studies of
small molecules with DNA are useful to understand the reaction mechanism and to
provide guidance for the application and design of new and more efficient drugs
or sensors targeted on DNA. In this study, the binding interaction between the
synthesized ligand and complex with calf thymus DNA (CT-DNA) has been
investigated using UV-Visible and emission DNA titration. From the UV-Visible
DNA study, it showed that platinum (II) bipyridine complex had higher affinity
towards CT-DNA with binding constant Kb=(3.1 ± 0.02 x 105)
± 0.02 M-1 compared to that of bis(phenoxy) bipyridine ligand with
binding constant (Kb ) = (1.19 ± 0.08) × 103 M-1.
These findings will be valuable for the potential use of platinum (II)
bipyridine complex as a phosphorescent probe in optical sensor DNA.
Keyword: bis(phenoxy)bipyridine,
intercalating ligand, calf thymus-DNA, DNA interaction
Abstrak
Ligan tetradentat dengan empat atom
penderma OONN dan kompleks platinumnya telah disintesis. Ligan
bis(fenoksi)bipiridin disediakan melalui tindak balas pengkupelan Suzuki antara
6,6’-dibromo-2,2’-bipiridil dan asid 2-hidroksifenilboronik dengan kehadiran
palladium(II) asetat. Pembentukan kompleks platinum dilakukan dengan
menambahkan platinum(II) klorida terhadap ligan di dalam benzonitril. Kedua-dua
struktur ligan dan kompleks dibuktikan dengan spekroskopi 1H, 2D dan
13C RMN, spektrometri ESI-MS dan spektroskopi FTIR. Kajian
pengikatan terhadap molekul kecil dengan DNA sangat berguna untuk memahami
mekanisme tindak balas dan untuk panduan bagi aplikasi dan reka bentuk dadah
yang baharu dan berkesan atau sensor target kepada DNA. Dalam kajian ini,
interaksi ikatan antara ligan dan kompleks terhadap DNA timus anak lembu
(CT-DNA) dilakukan dengan kajian titratan ultra lembayung boleh Nampak dan
titratan pendaflour. Kaedah tersebut membuktikan bahawa kompleks platinum (II)
bipiridin mempunyai ikatan yang lebih kuat terhadap CT-DNA berbanding dengan
ligan iaitu sebanyak Kb=(3.1 ± 0.02 x 105) ± 0.02 M-1
berbanding Kb=(1.19 ± 0.08) x 103 M-1.
Penemuan ini sangat bernilai dan berpotensi dalam menjadikan kompleks platinum (II)
bipiridin sebagai prob fosforesen dalam sensor DNA optik.
Kata kunci: bis(fenoksi)bipiridin, ligan berinterkalasi, DNA timus, interaksi DNA
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