Malaysian Journal of Analytical Sciences
Vol 20 No 1 (2016): 21 - 30
ENZYMATIC SACCHARIFICATION ON AMMONIA PRE-TREATED
OIL PALM TRUNK BIOMASS FOR GLUCOSE PRODUCTION:
AN OPTIMIZATION USING RESPONSE SURFACE
METHODOLOGY
(Sakarifikasi Enzim pada Prarawatan Ammonia Biomas Batang
Kelapa Sawit bagi Penghasilan Glukosa: Pengoptimuman dengan Kaedah Gerak Balas Permukaan)
Long Wee Lai*, Siti Sarenah Mohd Yahya, Norakma Mohd
Nor, Moohamad Ropaning Sulong
Faculty of
Science and Biotechnology,
Universiti
Selangor,
Jalan Timur
Tambahan, 45600 Bestari Jaya, Selangor, Malaysia
*Corresponding author: zki@unisel.edu.my
Received:
14 August 2015; Accepted: 31 December 2015
Abstract
This
paper reported the optimization of glucose production from ammonia pre-treated
oil palm trunk biomass via enzymatic saccharification process using response
surface methodology (RSM). A set of experiment was computed by face centered
central composite design (FCCCD) using Design Expert software to statistically
evaluate the findings. Three independent variables namely: temperature (35 – 55
°C), pH (4.5 – 6.5) and enzyme ratio (cellullase: β-glucosidase; 3:1 – 7:1) were investigated under the given
conditions designed by RSM. The experimental result (4.964 ± 0.006 g.L-1)
was in good agreement with RSM model prediction (4.958 g.L-1) with
an error less than 0.2 %. The RSM design has been proven to successfully
predict the glucose response in this study.
Keywords: oil palm trunk biomass, enzymatic
saccharification, lignocellulosic substrate, ammonia pre-treament,
response surface methodology
Abstrak
Kertas kerja ini melaporkan pengoptimuman hasilan
glukosa daripada prarawatan ammonia biomas
batang kelapa sawit melalui proses sakarifikasi enzim dengan menggunakan kaedah gerak balas permukaan (RSM). Satu set eksperimen telah
dibentuk iaitu kaedah muka berpusat reka bentuk komposit
dengan menggunakan perisian Design
Expert bagi menilai penemuan kajian secara statistik. Tiga pembolehubah bebas iaitu: suhu (35 – 55 ° C),
pH (4.5 – 6.5) dan nisbah enzim (selulase: β-glukosidase; 3:1 – 7:1) telah disiasat di
bawah situasi yang direka oleh
RSM. Hasil eksperimen
(4.964 ± 0.006 g.L-1) yang diperoleh berada dalam persetujuan
yang baik dengan ramalan model RSM (4.958 g.L-1) iaitu pada ralat kurang daripada 0.2 %. Reka
bentuk RSM telah terbukti berjaya meramalkan respon glukosa dalam kajian ini.
Kata kunci: biomas batang kelapa sawit, sakarifikasi enzim,
substrat lignoselulosa, pra-rawatan ammonia, kaedah gerak balas permukaan
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