Malaysian Journal of Analytical Sciences Vol 21 No 1 (2017): 213 - 220

DOI: http://dx.doi.org/10.17576/mjas-2017-2101-25

 

 

 

THE EFFECT OF GLUCOSE ADDITION IN ACETONE-BUTANOL-ETHANOL FERMENTATION FROM PALM OIL MILL EFFLUENT

BY Clostridium Acetobutylicum NCIMB 619

 

(Kesan Penambahan Glukosa dalam Fermentasi Aseton-Butanol-Etanol daripada Efluen Kilang Kelapa Sawit oleh Clostridium Acetobutylicum NCIMB 619)

 

Azima Syafaini Japar¹*, Mohd. Sobri Takriff², Jamaliah Md. Jahim¹, Abdul Amir Hassan Kadhum¹

 

¹Department of Chemical and Process Engineering

² Research Center for Sustainable Process Technology

Faculty of Engineering and Built Environment,

Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, Malaysia

 

*Corresponding author: azima.syafaini@siswa.ukm.edu.my

 

 

Received: 21 October 2015; Accepted: 14 June 2016

 

 

Abstract

This study investigates the production of acetone, butanol and ethanol (ABE) in sterilized sludge of palm oil mill effluent (POME) with the addition of glucose by Clostridium acetobutylicum NCIMB 619. Glucose concentrations of 5 g/L, 10 g/L and 15 g/L were used with volume percentage of 3% (v/v) and 5% (v/v) for each concentration. The highest ABE production of 9.89 g/L was observed with the addition of 10 g/L glucose with volume percentage of 5% (v/v) compared to 0.49 g/L in the control batch, with highest ABE productivity and yield, Y_P/S of 0.14 g/L/h and 1.36 g ABE/g substrate, respectively. Providing culture with 15 g/L glucose with volume percentage of 5% (v/v) was found to reduce the ABE production with lower productivity and ABE yield. The lower production at higher sugar level might be due to sugar inhibition that occurred during the process.

 

Keywords:  acetone-butanol-ethanol fermentation, palm oil mill effluent, Clostridium acetobutylicum, glucose addition

 

Abstrak

Kajian ini dilakukan bagi menentukan kesan penamabahan glukosa terhadap penghasilan aseton, butanol dan etanol (ABE) di dalam medium mendakan efluen kilang kelapa sawit (POME) steril oleh Clostridium acetobutylicum NCIMB 619. Kepekatan glukosa yang digunakan adalah 5 g/L, 10 g/L dan 15 g/L dengan peratus isipadu sebanyak 3% (v/v) dan 5% (v/v) bagi setiap kepekatan. Penghasilan ABE yang paling tinggi iaitu sebanyak 9.89 g/L diperoleh pada penambahan 10 g/L glukosa dengan peratus isipadu 5% (v/v) berbanding 0.49 g/L ABE yang diperoleh di dalam medium kawalan. Produktiviti dan hasil ABE yang diperoleh juga adalah yang tertinggi iaitu masing-masing sebanyak 0.14 g/L/j dan 1.36 g ABE/g substrat. Penambahan 15 g/L glukosa dengan peratus isipadu sebanyak 5% (v/v) didapati mengurangkan penghasilan ABE dengan produktiviti dan hasil ABE yang paling rendah disebabkan oleh perencatan gula yang berlaku semasa proses fermentasi.

 

Kata kunci:    fermentasi aseton-butanol-etanol, efluen kilang kelapa sawit, clostridium acetobutylicum, penambahan glukosa

 

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