 |
 |
 |
 |
 |
 |
Sains
Malaysiana 38(2): 191-196(2009)
Solvent Fermentation From Palm Oil Mill Effluent Using Clostridium acetobutylicum In Oscillatory Flow Bioreactor (Penghasilan Pelarut dari Fermentasi Efluen Loji Minyak Sawit oleh Klostridium Asetobutilikum di Dalam Bioreaktor Aliran Berayun)
M.S. Takriff, N. Masngut, A. A. H. Kadhum,
M. S. Kalil And A. W. Mohammad Department of Chemical &
Process Engineering
Faculty of Engineering,Universiti
Kebangsaan Malaysia
43600 Bangi. Selangor Darul
Ehsan, Malaysia
Received: 4 January 2008/Accepted: 11 Ogos 2008
ABSTRACT
Acetone-butanol-ethanol
(ABE) fermentation from Palm Oil Mill Effluent (POME) by C. acetobutylicum NCIMB 13357 in an oscillatory flow bioreactor was investigated.
Experimental works were conducted in a U-shaped stainless steel oscillatory
flow bioreactor at oscillation frequency between 0.45-0.78 Hz and a constant
amplitude of 12.5 mm. Fermentations were carried out for 72 hr at 35oC
using palm oil mill effluent and reinforced clostridia medium as a growth
medium in batch culture. Result of this investigation showed that POME is a
viable media for ABE fermentation and oscillatory flow bioreactor has an
excellent potential as an alternative fermentation device.
Keywords: Anaerobic fermentation; Clostridium
acetobutylicum; oscillatory flow bioreactor; palm Oil Mill Effluent;
solvent
ABSTRAK
Kajian ini fokus kepada penghasilan pelarut asiton, butanol dan etanol (ABE) daripada fermentasi efluen loji minyak sawit oleh C. Acetobutylicum di dalam bioreaktor aliran berayun. Ujikaji makmal telah dijalankan di dalam sebuah bioreaktor aliran berayun berbentuk U yang diperbuat dari keluli tahan karat. Kajian ini dilkasanakan pada julat frekuensi 0.45-0.78Hz dan amplitud malar 12.5 mm. Proses fermentasi dilaksanakan secara sesekumpul selama 72 jam pada suhu 35oC dengan menggunakan efluen loji minyak sawit (POME) dan media klostridia yang telah diperkukuhkan (RCM) sebagai medium tumbesaran. Keputusan kajian ini menunjukkan POME adalah media tumbesaran yang sesuai bagi penghasilan pelarut oleh C. Aketobutilikum dan bioreaktor aliran berayun menunjukan potensi yang sangat baik sebagai alat fermentasi alternatif.
Kata kunci: Bioreaktor aliran berayun; efluen loji minyak sawit; fermentasi anerobik; Kostridium asetobutulicum; pelarut
REFERENCES
Ahmad, A.L, Sumathi, S. & Hameed, B.H,
2006, Coagulation Of Residue Oil And Suspended Solid In Palm Oil Mill Effluent
By Chitosan, Alum And PAC, Chem. Eng, Jour. 118: 99-105.
Brunold, C.L., J.C.B. Hunns, M.R. Mackley &
J.W. Thomson. 1989. Experimental Observations On Flow Patterns And Energy Loses
For Oscillatory Flow In Ducts Containing Sharp Edges. Chem. Eng. Sci.
44: 1227-1244.
Dickens, A.W.,
M.R. Mackley & H.R. Williams. 1989. Experimental Residence Time
Distribution Measurements For Unsteady Flow In Baffled Tubes. Chem. Eng. Sci. 44 (7): 1471-1479.
Ezeji, T.C, Qureshi, N & Blaschek, H.P.
2007. Bioproduction Of Butanol From
Biomass: From Genes To Bioreactors, Current
Opinion In Biotechnology 18: 1-8.
Girbal, L &
Soucaille, P. 1998. Regulation Of Solvent Production In Clostridium Acetobutylicum. TIBTECH. 16: 11-16.
Harrisons, S.T.L. & M.R. Mackley. 1992. A
Pulsatile Flow Bioreactor. Chem. Eng. Sci. 47(2): 490-493.
Jones, D. T.
& Woods. D. R. 1986. Acetone-Butanol Fermentation Revisited. Microbiol. Rev. 50(94): 484-524.
Kalil, M. S.,
Kit, P.W., Yusoff, W. M. W., Sadazo, Y. & Rahman, R. A. 2003. Direct Fermentation
Of Palm Oil Mill Effluent To Acetone-Butanol-Ethanol Solvent Producing
Clostridia. Pakistan J. Biol Sci.
6(14): 1273-1275.
Lamed, R. J., Lobos, J. H. & Su, T. M. 1988. Effects Of Stirring Ad
Hydrogen On Fermentation Products Of Clostridium Thermocellum. App. Environ.
Microbiol. 54(5): 1216-1221.
Mackay, M.E.,
M.R. Mackley & Y. Wang. 1991. Oscillatory Flow Within Tubes Containing Wall
Or Central Baffles. Trans Icheme. 69(A): 506-513.
Miller, G.L.
1959. Use Of Dinitrosalicylic Acid Reagent For Determination Of Reducing Sugar. Anal. Chem. 31: 426.
Ni, X., H. Jian
& A. W. Fitch. 2002. Computanional Fluid Dynamic Modelling Of Flow Patterns
In An Oscillatory Baffled Column. Chem. Eng. Sci. 57: 2849-2862.
Ni, X., Gao,
S. & Pritchard, D. W. 1995. A Study Of Mass Transfer In Yeast In A Pulsed
Baffle Bioreactor. Biotechnol And Bioeng. 45: 165-175.
Somrutai, W., M. Takagi & T. Yoshida. 1996. Acetone-Butanol Fermentation By Clostridium Acetobutyricum ATCC 17777 From A Model Medium For Palm Oil Mill Effluent. J. Of Fermentation And Bioeng. 81(6): 543-547. Takriff, M.S. & Masyitah,
Z. 2002. Interstage Backmixing In Oscillatory Flow In A Baffled Column, Chemical Engineering Communications,
189(12): 1640-1652.
Ugoji, E.O. 1997. Anaerobic Digestion of Palm Oil Mill Effuene
and its Utilization as Fertilizer for Environmental/Roteltion, Renewable Energy
10(2): 20.
Vijayaragharan, K., A. Desa & Abdul Aziz, M.E. 2007. Aerobic Treatment of Palm Oil Mill Efflyent, Jour. Of Env. Mgmt. 82: 24-31.
|
|||
|
